Physical mapping of genes on yeast mitochondrial DNA: Localization of antibiotic resistance loci, and rRNA and tRNA genes

1978 ◽  
Vol 163 (3) ◽  
pp. 241-255 ◽  
Author(s):  
Richard Morimoto ◽  
Sylvie Merten ◽  
Alfred Lewin ◽  
Nancy C. Martin ◽  
Murray Rabinowitz
Keyword(s):  
Mitochondrial Dna ◽  
Antibiotic Resistance ◽  
Physical Mapping ◽  
Trna Genes ◽  
Yeast Mitochondrial Dna

Yeast mitochondrial DNA specifies tRNA for 19 amino acids. Deletion mapping of the tRNA genes

Biochemistry ◽  
1977 ◽  
Vol 16 (21) ◽  
pp. 4672-4677 ◽  
Author(s):  
Nancy C. Martin ◽  
Murray Rabinowitz ◽  
Hiroshi Fukuhara
Keyword(s):  
Amino Acids ◽  
Mitochondrial Dna ◽  
Trna Genes ◽  
Deletion Mapping ◽  
Yeast Mitochondrial Dna

scholarly journals Characterization of tRNA genes in tRNA region II of yeast mitochondrial DNA

1980 ◽  
Vol 8 (21) ◽  
pp. 5007-5016 ◽  
Author(s):  
Dawn Newman ◽  
Hung D. Pham ◽  
Karen Underbrink-Lyon ◽  
Nancy C. Martin
Keyword(s):  
Mitochondrial Dna ◽  
Trna Genes ◽  
Region Ii ◽  
Yeast Mitochondrial Dna

scholarly journals NEW ANTIBIOTIC RESISTANCE LOCI IN THE RIBOSOMAL REGION OF YEAST MITOCHONDRIAL DNA

Genetics ◽  
1980 ◽  
Vol 94 (1) ◽  
pp. 69-92
Author(s):  
Jeffrey A Knight
Keyword(s):  
Mitochondrial Dna ◽  
Antibiotic Resistance ◽  
Resistant Mutant ◽  
Cross Resistance ◽  
Antibiotic Resistant ◽  
Resistance Patterns ◽  
Genetic Techniques ◽  
Resistant Mutants ◽  
Yeast Mitochondrial Dna ◽  
Antibiotic Resistant Mutants

ABSTRACT A large number of mitochondrial antibiotic-resistant mutants have been isolated following mutagenesis with manganese. These include several different phenotypic classes of mutants, as distinguished by cross-resistance patterns, that have been found to be allelic at capl or eryl; some have been found to be heteroalle1ic.—Seven chloramphenicol-resistant mutants have been identified that are nonallelic by recombination tests with the three loci (capl, spil and eryl) previously identified in the ribosomal region. Four of these are allelic with each other and define a new locus, cap3; two others are allelic and define another new locus, cap2; the seventh maps at yet a different locus, cap4. One new spiramycin-resistant mutant has been identified that defines still another new locus, spi2. A variety of genetic techniques have been used to map these loci within the ribosomal region of the mitochondrial genome.—Manganese has been shown to be effective in inducing the mutation from ω- to ωn in many mutants that experience a simultaneous mutation at the closely linked capl locus. The ωn mutation has also been described in the capl mutant, and this locus has been shown to be more closely linked to o than capl is to ω.

scholarly journals Assembly of the mitochondrial membrane system. Physical map of the Oxi3 locus of yeast mitochondrial DNA.

1980 ◽  
Vol 255 (24) ◽  
pp. 11922-11926 ◽  
Author(s):  
S.G. Bonitz ◽  
G. Coruzzi ◽  
B.E. Thalenfeld ◽  
A. Tzagoloff ◽  
G. Macino
Keyword(s):  
Mitochondrial Dna ◽  
Mitochondrial Membrane ◽  
Physical Map ◽  
Membrane System ◽  
Yeast Mitochondrial Dna

scholarly journals Group II intron domain 5 facilitates a trans-splicing reaction.

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366 ◽  
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

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