Second-site antibiotic resistance mutations in the ribosomal region of yeast mitochondrial DNA

1982 ◽  
Vol 5 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Jeffrey A. Knight ◽  
Albert J. Courey ◽  
Barbara Stebbins
Keyword(s):  
Mitochondrial Dna ◽  
Antibiotic Resistance ◽  
Resistance Mutations ◽  
Antibiotic Resistance Mutations ◽  
Yeast Mitochondrial Dna

scholarly journals The fitness costs of antibiotic resistance mutations

2014 ◽  
Vol 8 (3) ◽  
pp. 273-283 ◽  
Author(s):  
Anita H. Melnyk ◽  
Alex Wong ◽  
Rees Kassen
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Mutations ◽  
Fitness Costs ◽  
Antibiotic Resistance Mutations

Antibiotic resistance mutations induced in growing cells of Bacillus-related thermophiles

2018 ◽  
Vol 71 (3) ◽  
pp. 382-389 ◽  
Author(s):  
Hirokazu Suzuki ◽  
Tatsunari Taketani ◽  
Jyumpei Kobayashi ◽  
Takashi Ohshiro
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Mutations ◽  
Antibiotic Resistance Mutations

scholarly journals Predicting nitroimidazole antibiotic resistance mutations in Mycobacterium tuberculosis with protein engineering

2020 ◽  
Vol 16 (2) ◽  
pp. e1008287 ◽  
Author(s):  
Brendon M. Lee ◽  
Liam K. Harold ◽  
Deepak V. Almeida ◽  
Livnat Afriat-Jurnou ◽  
Htin Lin Aung ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Mycobacterium Tuberculosis ◽  
Protein Engineering ◽  
Resistance Mutations ◽  
Antibiotic Resistance Mutations

scholarly journals Ribosomal Protein S14 of Saccharomyces cerevisiae Regulates Its Expression by Binding toRPS14B Pre-mRNA and to 18S rRNA

1999 ◽  
Vol 19 (1) ◽  
pp. 826-834 ◽  
Author(s):  
Sheara W. Fewell ◽  
John L. Woolford
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antibiotic Resistance ◽  
Ribosomal Protein ◽  
18S Rrna ◽  
Resistance Mutation ◽  
Resistance Mutations ◽  
Direct Demonstration ◽  
C Terminus ◽  
Antibiotic Resistance Mutations

ABSTRACT Production of ribosomal protein S14 in Saccharomyces cerevisiae is coordinated with the rate of ribosome assembly by a feedback mechanism that represses expression of RPS14B. Three-hybrid assays in vivo and filter binding assays in vitro demonstrate that rpS14 directly binds to an RNA stem-loop structure inRPS14B pre-mRNA that is necessary for RPS14Bregulation. Moreover, rpS14 binds to a conserved helix in 18S rRNA with approximately five- to sixfold-greater affinity. These results support the model that RPS14B regulation is mediated by direct binding of rpS14 either to its pre-mRNA or to rRNA. Investigation of these interactions with the three-hybrid system reveals two regions of rpS14 that are involved in RNA recognition. D52G and E55G mutations in rpS14 alter the specificity of rpS14 for RNA, as indicated by increased affinity for RPS14B RNA but reduced affinity for the rRNA target. Deletion of the C terminus of rpS14, where multiple antibiotic resistance mutations map, prevents binding of rpS14 to RNA and production of functional 40S subunits. The emetine-resistant protein, rpS14-EmRR, which contains two mutations near the C terminus of rpS14, does not bind either RNA target in the three-hybrid or in vitro assays. This is the first direct demonstration that an antibiotic resistance mutation alters binding of an r protein to rRNA and is consistent with the hypothesis that antibiotic resistance mutations can result from local alterations in rRNA structure.

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