Stimulation of direct embryogenesis from mesophyll protoplasts of Medicago sativa

1986 ◽  
Vol 5 (6) ◽  
pp. 468-470 ◽  
Author(s):  
M. Dijak ◽  
D. L. Smith ◽  
T. J. Wilson ◽  
D. C. W. Brown
Keyword(s):  
Medicago Sativa ◽  
Mesophyll Protoplasts ◽  
Direct Embryogenesis ◽  
Stimulation Of

Stimulation of Pancreatic Lipase Activity by Saponins Isolated from Medicago sativa L.

1997 ◽  
Vol 52 (3-4) ◽  
pp. 235-239 ◽  
Author(s):  
Zbigniew Sroka ◽  
Marian Jurzysta ◽  
Joanna Tylcz ◽  
Halina Rządkowska-Bodalska
Keyword(s):  
Medicago Sativa ◽  
Proteolytic Activity ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Aerial Part ◽  
Lipolytic Activity ◽  
Sodium Cholate ◽  
Pancreatic Enzymes ◽  
Medicago Sativa L ◽  
Stimulation Of

Abstract Saponins isolated from Medicago sativa L. appeared to stimulate lipolytic activity and did not influence amylolytic or proteolytic activity of Neopancreatinum (extract of pancreatic enzymes: lipase, proteases and amylases). Saponins isolated from the aerial part of Medicago sativa L. were demonstrated to stimulate lipase activity more effectively than saponins sepa­ rated from the root of this plant. When saponins were treated with 0.02 m HC1 at 37 °C for 1 hour their lipase stimulation ability did not change. The saponins stimulated Neopancreatinum lipolytic activity more in­ tensively in the presence of sodium cholate.

scholarly journals Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant, Medicago sativa (lucerne)

1997 ◽  
Vol 78 (2) ◽  
pp. 325-334 ◽  
Author(s):  
Alison M Gray ◽  
Peter R Flatt
Keyword(s):  
Insulin Secretion ◽  
Medicago Sativa ◽  
Aqueous Extract ◽  
Cumulative Effect ◽  
Abdominal Muscle ◽  
Heat Stable ◽  
Treatment Of Diabetes ◽  
Fold Stimulation ◽  
Plant Treatment ◽  
Stimulation Of

Medicago sativa (lucerne) is used as a traditional plant treatment of diabetes. In the present study, administration of lucerne in the diet (62·5 g/kg) and drinking water (2·5 g/l) reduced the hyperglycaemia of streptozotocin-diabetic mice. An aqueous extract of lucerne (1 mg/ml) stimulated 2-deoxy-glucose transport (1·8-fold), glucose oxidation (1·7-fold) and incorporation of glucose into glycogen (l·6-fold) in mouse abdominal muscle. In acute 20 min tests, 0·25–1 mg/ml aqueous extract of lucerne evoked a stepwise 2·5–6·3-fold stimulation of insulin secretion from the BRIN-BD11 pancreatic B-cell line. This effect was abolished by 0·5 mM-diazoxide, and prior exposure to extract did not affect subsequent stimulation of insulin secretion by 10 mM-L-alanine, thereby negating a detrimental effect on cell viability. The effect of extract was potentiated by 16·7 mM-glucose and by 1 mM-3-isobutyl-1-methylxanthine. L-Alanine (10 mM) and a depolarizing concentration of KCI (25 mM) did not augment the insulin-releasing activity of lucerne. Activity of the extract was found to be heat stable and largely acetone insoluble, and was enhanced by exposure to acid and alkali (0·1 M-HCI and NaOH) but decreased 25% with dialysis to remove components with molecular mass <2000 Da. Sequential extraction with solvents revealed insulin-releasing activity in both methanol and water fractions indicating a cumulative effect of more than one extract constituent. The results demonstrate the presence of antihyperglycaemic, insulin-releasing and insulin-like activity in the traditional antidiabetic plant, Medicago sativu.

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