A mutant of Escherichia coli auxotrophic for organic phosphates: Evidence for two defects in inorganic phosphate transport

1975 ◽  
Vol 143 (1) ◽  
pp. 71-77 ◽  
Author(s):  
George F. Sprague ◽  
Robert M. Bell ◽  
John E. Cronan
Keyword(s):  
Escherichia Coli ◽  
Inorganic Phosphate ◽  
Phosphate Transport ◽  
Organic Phosphates

scholarly journals Copper tolerance mediated by polyphosphate degradation and low-affinity inorganic phosphate transport system in Escherichia coli

2014 ◽  
Vol 14 (1) ◽  
pp. 72 ◽  
Author(s):  
Mariana Grillo-Puertas ◽  
Lici Schurig-Briccio ◽  
Luisa Rodríguez-Montelongo ◽  
María Rintoul ◽  
Viviana Rapisarda
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Inorganic Phosphate ◽  
Phosphate Transport ◽  
Copper Tolerance ◽  
Polyphosphate Degradation

scholarly journals Energy coupling to the transport of inorganic phosphate in Escherichia coli K12

1979 ◽  
Vol 178 (1) ◽  
pp. 133-137 ◽  
Author(s):  
H Rosenberg ◽  
R G Gerdes ◽  
F M Harold
Keyword(s):  
Escherichia Coli ◽  
Energy Source ◽  
Inorganic Phosphate ◽  
Energy Coupling ◽  
Phosphate Transport ◽  
Proton Motive Force ◽  
High Concentration ◽  
Concentration Gradients ◽  
Transport Of Inorganic Phosphate ◽  
Pst System

The nature of the energy source for phosphate transport was studied in strains of Escherichia coli in which either one of the two major systems (PIT, PST) for phosphate transport was present. In the PIT system, phosphate transport is coupled to the proton-motive force. The energy source for the PST system appears to be phosphate-bond energy, as has been found in other systems involving binding proteins. High concentration gradients of phosphate (between 100 and 500) are established by both systems.

Sodium, potassium, and intestinal transport of glucose, l-tyrosine, phosphate, and calcium

1963 ◽  
Vol 205 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Harold E. Harrison ◽  
Helen C. Harrison
Keyword(s):  
Small Intestine ◽  
Calcium Transport ◽  
Inorganic Phosphate ◽  
Choline Chloride ◽  
Sodium Concentration ◽  
Phosphate Transport ◽  
Metabolic Energy ◽  
Transport Systems ◽  
Bicarbonate Buffer ◽  
Distal Small Intestine

Everted loops of rat small intestine were incubated in media varying in their concentrations of sodium and potassium. Reduction of sodium concentration was effected by substitution of choline chloride in equimolar amounts for sodium chloride in the saline-bicarbonate buffer. Concentrative transport of glucose, l-tyrosine, inorganic phosphate, and calcium was measured by determination of the final ratio of the concentrations of the solute in serosal and mucosal fluids, and the increment of the solute in serosal fluid during incubation. Ca45 was used as an indicator of calcium distribution. The glucose, l-tyrosine, and inorganic phosphate transport systems require sodium, and at a submaximal concentration of sodium an increased concentration of potassium is inhibitory. The calcium transport system does not require sodium and in loops from the distal small intestine calcium transport is enhanced by reduction of sodium concentration in the medium. It is postulated that there is a common sodium-requiring system which is necessary for the linkage of metabolic energy to glucose, amino acid, and inorganic phosphate transport.

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