Use of an electrochemical technique to study plasmamembrane redox reactions in cultured cells of Daucus carota L.

1984 ◽  
Vol 3 (6) ◽  
pp. 243-246 ◽  
Author(s):  
J. D. C. Chalmers ◽  
J. O. D. Coleman ◽  
N. J. Walton
Keyword(s):  
Daucus Carota ◽  
Redox Reactions ◽  
Cultured Cells ◽  
Electrochemical Technique ◽  
Daucus Carota L

Characterization and Properties of Different Glucosyltransferases Isolated from Suspension-Cultured Cells of Daucus carota

1986 ◽  
Vol 41 (4) ◽  
pp. 409-420 ◽  
Author(s):  
Edgar Ingold ◽  
Hanns Ulrich Seitz
Keyword(s):  
Daucus Carota ◽  
Cultured Cells ◽  
Test System ◽  
Enzymatic Activities ◽  
Daucus Carota L ◽  
Low Concentrations ◽  
Nucleotide Sugars ◽  
Glucan Chain ◽  
Glucan Synthesis

Particulate enzymes (14,000 g pellet) from suspension-cultured carrot cells (Daucus carota L.) incorporated glucose from UDP-glucose and GDP-glucose into ethanol-insoluble products which were characterized as glucans or glucoprotein. Based on the test system to assay glucansynthe- tases I and II four different enzymatic activities could be distinguished on the basis of their substrate and divalent cation requirements, the influence of active substances such as nucleotides, nucleotide sugars, cellobiose, and in vivo inhibitors of cell wall glucan synthesis, their distribution in linear sucrose gradient and the nature of their products. The enzymatic activities which incor­porated glucose from UDP-glucose or GDP-glucose at low substrate concentrations (10 -6 ᴍ) were both localized in membranes of a density of 1.129 g em-3 (Golgi membranes) and synthesized a β-1,4-glucan chain. Both showed similar properties in most of the characterization experiments. The glucosyltransferase that catalysed the formation of a β-1,3-glucan from UDP-glucose (0.48 mᴍ) was found in membranes which accumulated at a density of 1.170 g · cm-3 (plasma membrane) and differed in its properties from the Golgi-localized glucosyltransferase activities in many aspects. A soluble glucosyltransferase (175,000 × g supernatant) which was also active at low concentrations of UDP-glucose (10-6 ᴍ) but showed enhanced activity under conditions where the other glucosyltransferases were inactive incorporated glucose into a proteinase-sensi­tive product. In linear sucrose gradients this enzyme migrated to different gradient densities depending on conditions.

Observations on growth and morphogenesis in cultured cells of carrot ( Daucus carota L.)

1975 ◽  
Vol 273 (922) ◽  
pp. 33-53 ◽  
Keyword(s):  
Thin Films ◽  
Somatic Embryogenesis ◽  
Special Reference ◽  
Daucus Carota ◽  
Liquid Culture ◽  
Cultured Cells ◽  
Daucus Carota L ◽  
Practical Applications ◽  
Carrot Cells ◽  
Different Levels

This paper deals with problems of somatic embryogenesis (totipotency) of carrot cells in liquid culture and when dispersed in thin films of nutrient agar. The critical events that intervene between somatic cells and embryo plantlets during morphogenesis are delineated. Factors that affect the course of these events are described with special reference to the stimuli which permit the very smallest units to develop into organized structures. Special attention is directed to the importance of a protracted period of darkness and the use of an ‘embryo-conditioned medium’ as interacting stimuli that facilitate the early stages of development. Means of arresting and releasing the development of pro-embryonic units are discussed. Aspects of growth and form of the developing cultures are presented photographically at different levels. The behaviour of free protoplasts from pro-embryonic units is described and contrasted with that of intact totipotent cells. The significance of all these observations is examined in the light of practical applications and in relation to problems of development.

scholarly journals Amperometric Measurement of Reducing Activity Exhibited by Intact Cultured Cells of Daucus carota L.

1995 ◽  
Vol 63 (12) ◽  
pp. 1171-1172 ◽  
Author(s):  
Toshiyasu KUROSAKI ◽  
Tokuji IKEDA ◽  
Kenji KANO ◽  
Naoki TANIGUCHI ◽  
Yukitaka YAMAMOTO ◽  
...  
Keyword(s):  
Daucus Carota ◽  
Cultured Cells ◽  
Daucus Carota L ◽  
Reducing Activity

DAYA TERIMA JAJANAN LOKAL BARONCONG DENGAN PENAMBAHAN WORTEL (DAUCUS CAROTA L)

2019 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Suriani Rauf ◽  
Manjilala Manjilala ◽  
Elvi Kusuma
Keyword(s):  
Experimental Study ◽  
Daucus Carota ◽  
Vegetable Consumption ◽  
Negative Impact ◽  
Local Foods ◽  
Ministry Of Health ◽  
Daucus Carota L

The behavior of people who less consume vegetables and fruit can have a negative impact on health. The lack of appetite for vegetable consumption by the society made the researchers interested in making a combination of vegetables, namely carrots with local foods, namely Baroncong.This research aims to determine the acceptability of local baroncong snacks with the addition of carrots.This research is a pre-experimental study. The panelists from this study consisted of 30 Nutrition Department Students from Health Polytechnic Ministry of Health Makassar. Assessment based on taste aspects, color of aroma and texture of baroncong products with the addition of carrots 50%, 75%, 100% with the criteria of very like, like, dislike and very dislike.The results showed that the acceptability of baroncong with the addition of carrots in terms of taste was a concentration of 75%, which was very much as much as 46.7% and liked as much as 50%, the color aspect was 50% concentration consisting of 36.7% likes and likes as much as 53.3%, the aroma aspectis a concentration of 50% which consists of very much as much as 33.3% and likes as much as 63.3%, in terms of aspects of texture are concentrations of 50% and 75%, each of which consists of as much as 23.3% and likes as much as 63, 3%. Based on these data, Baroncong with the addition of carrots received is a concentration of 75%.

ESTUDO DA CINÉTICA DE SECAGEM EM CENOURAS (DAUCUS CAROTA L.)

2018 ◽  
Author(s):  
Araújo Thaís Jaciane ◽  
Santos Newton Carlos ◽  
Barros Sâmela Leal ◽  
Melo Mylena Olga Pessoa ◽  
Nascimento Amanda Priscila Silva
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L

Anthocyanin accumulation and PAL activity in a suspension culture of Daucus carota L.

Planta ◽  
1980 ◽  
Vol 149 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Wolfgang No� ◽  
Christian Langebartels ◽  
Hanns Ulrich Seitz
Keyword(s):  
Suspension Culture ◽  
Daucus Carota ◽  
Anthocyanin Accumulation ◽  
Daucus Carota L ◽  
Pal Activity
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