Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume

1986 ◽  
Vol 5 (3) ◽  
pp. 174-177 ◽  
Author(s):  
L. Szabados ◽  
W. M. Roca
Keyword(s):  
Cell Suspension ◽  
Forage Legume ◽  
Stylosanthes Guianensis ◽  
Tropical Forage

Somaclonal variation in plant adaptation to acid soil in the tropical forage legume Stylosanthes guianensis

1992 ◽  
Vol 146 (1-2) ◽  
pp. 21-30 ◽  
Author(s):  
I. M. Rao ◽  
W. M. Roca ◽  
M. A. Ayarza ◽  
E. Tabares ◽  
R. Garcia
Keyword(s):  
Somaclonal Variation ◽  
Acid Soil ◽  
Forage Legume ◽  
Plant Adaptation ◽  
Stylosanthes Guianensis ◽  
Tropical Forage

scholarly journals Transient gene expression in electroporated intact tissues of Stylosanthes guianensis (Aubl.) Sw.

2001 ◽  
Vol 58 (4) ◽  
pp. 759-765 ◽  
Author(s):  
Vera Maria Quecini ◽  
Maria Lúcia Carneiro Vieira
Keyword(s):  
Gene Expression ◽  
Electric Field ◽  
Plasmid Dna ◽  
Transient Gene Expression ◽  
Pcr Amplification ◽  
Forage Legume ◽  
Stylosanthes Guianensis ◽  
Specific Pcr ◽  
Tropical Forage ◽  
Commercially Important

Genetic transformation though protoplast electroporation has been established for commercially important plant species. In this work, explant sources, electric field strengths, electroporation buffers, DNA forms and osmotic pretreatment were assayed in order to optimize transient reporter gene expression in electroporated tissues of Stylosanthes guianensis, a tropical forage legume. Higher transformation rates were obtained employing cotyledonary explants and an electric field strength of 250 V cm-1. Linear plasmid DNA, chloridefree electroporation buffer and osmotic pretreatment with 1.6 mol L-1 mannitol also improved transient transformation but non-significantly. Transgene specific PCR amplification was employed to prove the transformed status of the tissues.

Isolation and characterization of microsatellite loci in the tropical forage legume Stylosanthes guianensis (Aubl.) Sw.

2009 ◽  
Vol 1 (1) ◽  
pp. 43-46 ◽  
Author(s):  
M. O. Santos ◽  
C. T. Karia ◽  
R. M. S. Resende ◽  
L. Chiari ◽  
L. Jungmann ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Forage Legume ◽  
Stylosanthes Guianensis ◽  
Isolation And Characterization ◽  
Tropical Forage

scholarly journals Stomatal Development and Conductance of a Tropical Forage Legume Are Regulated by Elevated [CO2] Under Moderate Warming

2019 ◽  
Vol 10 ◽  
Author(s):  
Eduardo Habermann ◽  
Eduardo A. Dias de Oliveira ◽  
Daniele Ribeiro Contin ◽  
Juca A. B. San Martin ◽  
Lucas Curtarelli ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Forage Legume ◽  
Stomatal Development ◽  
Tropical Forage

scholarly journals Nutrient intake, digestibility and nitrogen balance of two temperate and two tropical forage legume hays fed to New Zealand white rabbits

2019 ◽  
Vol 46 (1) ◽  
pp. 132-142
Author(s):  
T. T. Akpensuen ◽  
J. T. Amodu ◽  
R. J. Tanko ◽  
S. B. Abdu ◽  
O. A. T. Namo ◽  
...  
Keyword(s):  
New Zealand ◽  
Trifolium Repens ◽  
Crude Protein ◽  
Dry Matter ◽  
The Other ◽  
Forage Legume ◽  
Forage Legumes ◽  
Crude Fibre ◽  
Tropical Forage ◽  
New Zealand White Rabbits

An experiment was carried out to evaluate two temperate forage legumes (Trifolium pratense and Trifolium repens) and two tropical forage legumes (Stylosanthes guianensis and Centrosema molle) for hay intake and digestibility in the cool tropical climate of Vom, Jos, Plateau State of Nigeria. The legumes were planted in the month of June, 2016 and harvested at early-podding stage for hay. Sixteen (16) growing male New Zealand White rabbits were used for the digestibility study. The rabbits were arranged in a Completely Randomised Design and in individual cages measuring 55 cm x 39 cm x 40 cm. Nutrient contents, intake and digestibility in rabbits were determined. Crude protein content was significantly (P<0.01) higher in T. repens (18.38%) compared to T. pratense (16.50 %), whereas S. guianensis produced the least value of 13.83%. The crude fibre content of the hay was significantly higher (P<0.0I) in S. guianensis (37.29 %) compared to the other forage legumes. Dry matter intake was significantly (P<0.01) higher in rabbits fed T. repens (77.88%) compared to T. pratense (76.57%), and the lowest value of 62.97% was obtained in S. guianensis. Crude protein intake was significantly (P<0.01) higher in rabbits fed T. repens (15.05 g d-1) compared to those fed T. pratense (12.71 g d-1), but S. guianensis had the least value of 8.64 g d-1. On the other hand, rabbits fed S. guianensis hay had a higher (P<0.01) crude fibre intake (23.39%). Trifolium repens was significantly (P<0.01) higher in dry matter and crude protein digestibility (64.79 and 85. 88 %), while S. guianensis had lower values of 56.89 and 71.33%, respectively. Trifolium repens also had a significantly (P<0.01) higher total nitrogen intake and retained compared to the other forage legumes. Nitrogen retained in T. repens was 2.40 g d-1 compared to 1.87 g d-1 in T. pretense, while S. guianensis had the lowest of 1.34 g d-1. The temperate forage legume hays were higher in nutrient content, intake and digestibility compared to the tropical legume hays. Therefore, the forage crops may serve as better supplementary feeds for livestock feeding especially during the dry season.

Sequence and Expression of a Caffeic Acid O-Methyl Transferase Cdna Homologue in the Tropical Forage Legume Stylosanthes Humilis.

1995 ◽  
Vol 22 (3) ◽  
pp. 471 ◽  
Author(s):  
CL Mcintyre ◽  
AL Rae ◽  
MD Curtis ◽  
JM Manners
Keyword(s):  
Amino Acids ◽  
Caffeic Acid ◽  
Open Reading Frame ◽  
Stem Tissue ◽  
Forage Legume ◽  
Methyl Transferase ◽  
Reading Frame ◽  
Stylosanthes Humilis ◽  
Tropical Forage ◽  
Young Leaves

The isolation and characterisation of a cDNA encoding a caffeic acid 0-methyl transferase cDNA homologue (COMT) from Stylosanthes humilis are described. The clone is 1391 nucleotides in length, with an open reading frame encoding a predicted protein of 366 amino acids. Cluster analysis of the deduced amino acid sequence revealed extensive homology to other published O-methyl transferase sequences. Maximum levels of homology were seen with COMTs from alfalfa (87%) and aspen (84%). Southern analysis suggested that this enzyme is encoded by two genes in S. humilis. The mRNA is most strongly expressed in stem tissue, with intermediate levels of expression in young leaves and roots, and does not appear to be induced upon fungal infection or wounding.

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