Phylogenetic relationships of Triticum tauschii, the D genome donor to hexaploid wheat

1989 ◽  
Vol 77 (6) ◽  
pp. 851-856 ◽  
Author(s):  
E. S. Lagudah ◽  
G. M. Halloran
Keyword(s):  
Hexaploid Wheat ◽  
Phylogenetic Relationships ◽  
D Genome ◽  
Triticum Tauschii ◽  
Genome Donor

Establishment of Fine Suspension Cultures of Triticum tauschii ([Coss.] Schmal.) which remain Embryogenic for Several Years

1999 ◽  
Vol 47 (4) ◽  
pp. 611
Author(s):  
Shoukat Afshar-Sterle ◽  
James F. Kollmorgen ◽  
Geoffrey B. Fincher
Keyword(s):  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
D Genome ◽  
Triticum Tauschii ◽  
Initiation Phase ◽  
Genome Donor ◽  
Embryogenic Suspension ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Term Maintenance

Immature embryos of 10 accessions of Triticum tauschii, the D genome donor of Triticum aestivum, were used to produce embryogenic callus for the initiation of suspension cultures. For the long-term maintenance of embryogenicity of these suspensions, it was necessary to use different media for the initiation, establishment and maintenance phases. The initiation phase required media supplemented with L-proline, L-asparagine and L-glutamine, together with Dicamba at 12 mg L −1 and maltose. In the establishment phase, it was essential to reduce the concentration of Dicamba to 6 mg L −1 for the rapid production of fine suspension cultures. Finally, the long-term maintenance of a capacity for regeneration depended on the inclusion of 1.1 mg L −1 2,4-dichlorophenoxyacetic acid and 30 g L −1 sucrose in the medium. By the use of these procedures, long-term embryogenic fine suspension cultures were established from two accessions, while non-embryogenic fine suspension cultures were produced from five accessions. Over 90% of plants regenerated from fine suspensions of 1-year-old embryogenic cultures were fertile, and embryogenic suspension cultures retained their regeneration capacity for more than 3 years.

A complex arrangement of genes at a starch branching enzyme I locus in the D-genome donor of wheat

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 465-474 ◽  
Author(s):  
S. Rahman ◽  
M. Morell ◽  
R. Appels ◽  
S. Abrahams ◽  
D. Abbott ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
D Genome ◽  
Triticum Tauschii ◽  
Putative Protein ◽  
Genome Donor ◽  
Opposite Strand ◽  
Enzyme I

Genomic DNA fragments from Triticum tauschii (D-genome donor to wheat) carrying starch branching enzyme I (SBE I) type genes have been characterized. One fragment contains one complete gene and two partial genes in 16 kb of DNA. One of the partial genes is oriented in the opposite strand to the other two. The gene that is complete was sequenced. Its structure corresponds closely to that of rice in that exons 3–8 are retained at similar sizes and spacings. A cDNA closely corresponding to the complete gene was isolated and characterized; it codes for a putative protein that represents a novel type of SBE I, as it is shorter at the 3′ end than the forms reported so far in other plants. A second genomic fragment contains a different SBE I gene. There appear to be approximately 10 copies of SBE I type genes in wheat (approximately 5 in T. tauschii) and most of them have been assigned to group 7 chromosomes. In situ hybridization indicates that a major locus for the genes is located at the distal end of the short arm of chromosome 7D.Key words: starch, branching enzyme genes, wheat, Triticum tauschii, in situ hybridization.

Phylogenetic relationships of Triticum tauschii, the D-genome donor to hexaploid wheat. 4. Variation and chromosomal location of 5S DNA

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1017-1025 ◽  
Author(s):  
E. S. Lagudah ◽  
B. C. Clarke ◽  
R. Appels
Keyword(s):  
Dna Sequences ◽  
Hexaploid Wheat ◽  
Chromosomal Location ◽  
Thermal Dissociation ◽  
D Genome ◽  
Triticum Tauschii ◽  
Main Cluster ◽  
Chinese Spring Wheat ◽  
Close Phylogenetic Relationship ◽  
5S Dna

The 5S DNA sequences in Triticum tauschii are organised in large clusters containing units that are primarily either 420 ("short") or 490 base pairs (bp) in length ("long"). The main cluster of short units was shown to be located on chromosome 1D in hexaploid wheat and is designated 5SDna-Dl, while the cluster of long units was shown to be on chromosome 5D and is designated 5SDna-D2. The chromosomal locations in hexaploid wheat most likely correspond to those in T. tauschii and this could be shown directly for the 5SDna-D2 locus by using a T. tauschii 5D substitution in 'Chinese Spring' wheat. The sequence alignment of units derived from 5SDna-D1 and 5SDna-D2 revealed three apparent deletions in the noncoding spacer region, which were fixed in units from 5SDna-D1, and one deletion, which was fixed in units from 5SDna-D2. A minor size class, 400 bp long and closely related to the units from 5SDna-D1, was found in 2 of 415 accessions surveyed. A continuous range of quantitative changes in the number of 5S DNA units at the two loci was evident with up to a 10-fold relative abundance level of units being found in some accessions. Triticum tauschii var. typica was particularly noteworthy in that many accessions showed more units at 5SDna-D2 relative to 5SDna-D1. Partial thermal dissociation experiments with radioactive probes, synthesized from either the short or long 5S DNA units, hybridized to genomic DNA showed that the population of units at the respective loci were relatively homogeneous and clearly distinct from each other. In addition, these experiments further established the close phylogenetic relationship between T. tauschii and the D genome of wheat.Key words: Triticum tauschii, 5S DNA, sequence variation, chromosomal location.

scholarly journals Genetic and Physiological Architecture of Early Vigor in Aegilops tauschii, the D-Genome Donor of Hexaploid Wheat. A Quantitative Trait Loci Analysis

2005 ◽  
Vol 139 (2) ◽  
pp. 1078-1094 ◽  
Author(s):  
Margreet W. ter Steege ◽  
Franka M. den Ouden ◽  
Hans Lambers ◽  
Piet Stam ◽  
Anton J.M. Peeters
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Hexaploid Wheat ◽  
Aegilops Tauschii ◽  
D Genome ◽  
Genome Donor ◽  
Trait Loci ◽  
Early Vigor

Highly Recombinogenic Regions at Seed Storage Protein Loci on Chromosome 1DS of Aegilops tauschii, the D-Genome Donor of Wheat

Genetics ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 361-367 ◽  
Author(s):  
Wolfgang Spielmeyer ◽  
Odile Moullet ◽  
André Laroche ◽  
Evans S Lagudah
Keyword(s):  
Genetic Distance ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Aegilops Tauschii ◽  
Bac Library ◽  
Seed Storage ◽  
D Genome ◽  
Rflp Map ◽  
Genome Donor ◽  
The Relationship

Abstract A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii, the diploid D-genome donor species of hexaploid wheat. Ae. tauschii was used to overcome some of the limitations commonly associated with molecular studies of wheat such as low levels of DNA polymorphism. Detection of multiple loci by most RFLP probes suggests that gene duplication events have occurred throughout this chromosomal region. Large DNA fragments isolated from a BAC library of Ae. tauschii were used to determine the relationship between physical and genetic distance at seed storage protein loci located at the distal end of chromosome 1DS. Highly recombinogenic regions were identified where the ratio of physical to genetic distance was estimated to be <20 kb/cM. These results are discussed in relation to the genome-wide estimate of the relationship between physical and genetic distance.

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