A complex arrangement of genes at a starch branching enzyme I locus in the D-genome donor of wheat

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 465-474 ◽  
Author(s):  
S. Rahman ◽  
M. Morell ◽  
R. Appels ◽  
S. Abrahams ◽  
D. Abbott ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
D Genome ◽  
Triticum Tauschii ◽  
Putative Protein ◽  
Genome Donor ◽  
Opposite Strand ◽  
Enzyme I

Genomic DNA fragments from Triticum tauschii (D-genome donor to wheat) carrying starch branching enzyme I (SBE I) type genes have been characterized. One fragment contains one complete gene and two partial genes in 16 kb of DNA. One of the partial genes is oriented in the opposite strand to the other two. The gene that is complete was sequenced. Its structure corresponds closely to that of rice in that exons 3–8 are retained at similar sizes and spacings. A cDNA closely corresponding to the complete gene was isolated and characterized; it codes for a putative protein that represents a novel type of SBE I, as it is shorter at the 3′ end than the forms reported so far in other plants. A second genomic fragment contains a different SBE I gene. There appear to be approximately 10 copies of SBE I type genes in wheat (approximately 5 in T. tauschii) and most of them have been assigned to group 7 chromosomes. In situ hybridization indicates that a major locus for the genes is located at the distal end of the short arm of chromosome 7D.Key words: starch, branching enzyme genes, wheat, Triticum tauschii, in situ hybridization.

Erratum: A complex arrangement of genes at a starch branching enzyme I locus in the D-genome donor of wheat

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 139
Author(s):  
S Rahman ◽  
S Abrahams ◽  
D Abbott ◽  
Y Mukai ◽  
M Samuel ◽  
...  
Keyword(s):  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
D Genome ◽  
Genome Donor ◽  
Enzyme I ◽  
Complex Arrangement

C-banding polymorphisms in several accessions of Triticum tauschii (Aegilops squarrosa)

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 192-199 ◽  
Author(s):  
B. Friebe ◽  
Y. Mukai ◽  
B. S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Banding Pattern ◽  
Chromosome Analysis ◽  
Polymorphic Variation ◽  
D Genome ◽  
Minor Variation ◽  
Triticum Tauschii ◽  
C Banding ◽  
Aegilops Squarrosa

A generalized C-banded karyotype of Triticum tauschii (Aegilops squarrosa) was established based on chromosome analysis of 15 accessions of diverse origin, including the two varieties eusquarrosa (with the former varieties typica, anathera, and meyeri) and strangulata. The C-banding pattern of T. tauschii chromosomes was similar to the D-genome chromosomes of cultivated bread wheat, T. aestivum, thus permitting their unequivocal identification and homoeologous chromosome designations. Whereas only minor variation in C-banding pattern was observed within the accessions, a large amount of polymorphic variation was found among the different accessions. However, this polymorphic variation did not prevent chromosome identification in these lines. One accession (TA 2462) was found to be homozygous for a reciprocal translocation involving the complete arms of chromosomes 1D and 7D. In situ hybridization using the D-genome specific probe pAS1 confirmed the presence of T1DS-7DL and T7DS.1DL translocation in the accession TA 2462.Key words: Triticum tauschii, C-banding, in situ hybridization.

Exploring the origin of the D genome of oat by fluorescence in situ hybridization

Genome ◽  
2014 ◽  
Vol 57 (9) ◽  
pp. 469-472 ◽  
Author(s):  
Xiaomei Luo ◽  
Haiqin Zhang ◽  
Houyang Kang ◽  
Xing Fan ◽  
Yi Wang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Avena Sativa ◽  
Genetic Improvement ◽  
Similar Distribution ◽  
Diploid Progenitor ◽  
D Genome ◽  
Genome Donor ◽  
Sequential Experiments

Further understanding of the origin of cultivated oat would accelerate its genetic improvement. In particular, it would be useful to clarify which diploid progenitor contributed the D genome of this allohexaploid species. In this study, we demonstrate that the landmarks produced by fluorescence in situ hybridization (FISH) of species of Avena using probes derived from Avena sativa can be used to explore the origin of the D genome. Selected sets of probes were hybridized in several sequential experiments performed on exactly the same chromosome spreads, with multiple probes of cytological preparations. Probes pITS and A3-19 showed there might be a similar distribution of pITS between the Ac and D genomes. These results indicated that the Ac genome is closely related to the D genome, and that Avena canariensis (AcAc) could be the D-genome donor of cultivated oat.

The starch branching enzyme I locus from Aegilops tauschii, the donor of the D genome to wheat

2003 ◽  
Vol 3 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Go Suzuki ◽  
Masaki Moriyama ◽  
Kumiko Fujioka ◽  
Maki Yamamoto ◽  
Nekkalapudi C. Subrahmanyam ◽  
...  
Keyword(s):  
Aegilops Tauschii ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
D Genome ◽  
Enzyme I

Chromosomal organization of a sequence related to LTR-like elements of Ty1-copia retrotransposons in Avena species

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 706-713 ◽  
Author(s):  
Concha Linares ◽  
Antonio Serna ◽  
Araceli Fominaya
Keyword(s):  
In Situ Hybridization ◽  
Diploid Species ◽  
D Genome ◽  
Specific Sequence ◽  
Chromosomal Organization ◽  
Intergenomic Translocations ◽  
A Genome ◽  
Slot Blot Analysis ◽  
Copia Retrotransposons

A repetitive sequence, pAs17, was isolated from Avena strigosa (As genome) and characterized. The insert was 646 bp in length and showed 54% AT content. Databank searches revealed its high homology to the long terminal repeat (LTR) sequences of the specific family of Ty1-copia retrotransposons represented by WIS2-1A and Bare. It was also found to be 70% identical to the LTR domain of the WIS2-1A retroelement of wheat and 67% identical to the Bare-1 retroelement of barley. Southern hybridizations of pAs17 to diploid (A or C genomes), tetraploid (AC genomes), and hexaploid (ACD genomes) oat species revealed that it was absent in the C diploid species. Slot-blot analysis suggested that both diploid and tetraploid oat species contained 1.3 × 104 copies, indicating that they are a component of the A-genome chromosomes. The hexaploid species contained 2.4 × 104 copies, indicating that they are a component of both A- and D-genome chromosomes. This was confirmed by fluorescent in situ hybridization analyses using pAs17, two ribosomal sequences, and a C-genome specific sequence as probes. Further, the chromosomes involved in three C-A and three C-D intergenomic translocations in Avena murphyi (AC genomes) and Avena sativa cv. Extra Klock (ACD genomes), respectively, were identified. Based on its physical distribution and Southern hybridization patterns, a parental retrotransposon represented by pAs17 appears to have been active at least once during the evolution of the A genome in species of the Avena genus.Key words: chromosomal organization, in situ hybridization, intergenomic translocations, LTR sequence, oats.

scholarly journals Comparison of starch branching enzyme I and II from potato

2001 ◽  
Vol 268 (23) ◽  
pp. 6140-6145 ◽  
Author(s):  
Ulrika Rydberg ◽  
Lena Andersson ◽  
Roger Andersson ◽  
Per Åman ◽  
Håkan Larsson
Keyword(s):  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Enzyme I

Molecular Characterization of Mungbean (Vigna radiataL.) Starch Branching Enzyme I

2010 ◽  
Vol 58 (19) ◽  
pp. 10437-10444 ◽  
Author(s):  
Jia-Wei Chang ◽  
Sing-Chung Li ◽  
Yun-Chi Shih ◽  
Reuben Wang ◽  
Pei-Shan Chung ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Enzyme I
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