Region-specific aldehyde oxidase activity in Tumorous-head eye discs of Drosophila melanogaster

1978 ◽  
Vol 163 (2) ◽  
pp. 125-129 ◽  
Author(s):  
David T. Kuhn ◽  
Franklin C. Walker
Keyword(s):  
Drosophila Melanogaster ◽  
Oxidase Activity ◽  
Aldehyde Oxidase

scholarly journals LACK OF DOSAGE COMPENSATION FOR AN AUTOSOMAL GENE RELOCATED TO THE X CHROMOSOME IN DROSOPHILA MELANOGASTER

Genetics ◽  
1977 ◽  
Vol 85 (3) ◽  
pp. 489-496
Author(s):  
Richard L Roehrdanz ◽  
James M Kitchens ◽  
John C Lucchesi
Keyword(s):  
Drosophila Melanogaster ◽  
Experimental Evidence ◽  
Structural Gene ◽  
X Chromosome ◽  
Dosage Compensation ◽  
Oxidase Activity ◽  
Aldehyde Oxidase ◽  
Autosomal Gene

ABSTRACT Aldehyde oxidase activity has been measured in flies with the structural gene for this enzyme translocated to the X chromosome. These measurements are presented as experimental evidence that, in Drosophila melanogaster, an autosomal gene relocated to the X chromosome is not dosage compensated.

THE EFFECTS OF LAO ON ALDEHYDE OXIDASE ACTIVITY AND CROSS-REACTING-MATERIAL IN DROSOPHILA MELANOGASTER

1978 ◽  
Vol 20 (4) ◽  
pp. 545-553 ◽  
Author(s):  
John H. Williamson ◽  
Michael M. Bentley ◽  
Melvin J. Oliver ◽  
Billy W. Geer
Keyword(s):  
Drosophila Melanogaster ◽  
Oxidase Activity ◽  
Mutant Allele ◽  
Aldehyde Oxidase ◽  
Wild Type ◽  
Heat Labile ◽  
Major Form ◽  
Normal Enzyme ◽  
Mutant Stock

In Drosophila melanogaster aldehyde oxidase occurs in at least two forms that can be separated electrophoretically. The mutant allele lao (low aldehyde oxidase activity) causes a deficiency of the major form of this enzyme. Immunoelectrophoretic analyses suggest that lao homozygotes produce aldehyde oxidase cross-reacting-material in nearly wild-type levels. Although aldehyde oxidase from the mutant stock is heat labile, properties such as Km and pH optima are not different from the normal enzyme.

GENETIC CONTROL OF ALDEHYDE OXIDASE ACTIVITY AND CROSS-REACTING-MATERIAL IN DROSOPHILA MELANOGASTER

1978 ◽  
Vol 20 (4) ◽  
pp. 489-497 ◽  
Author(s):  
Eva M. Meidinger ◽  
John H. Williamson
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Control ◽  
Structural Gene ◽  
Oxidase Activity ◽  
Aldehyde Oxidase ◽  
Xanthine Dehydrogenase ◽  
The Other ◽  
Pyridoxal Oxidase

Four different genes are known to affect aldehyde oxidase activity (AO) in Drosophila melanogaster. Mutants at each of these loci eliminate AO activity and simultaneously eliminate detectable AO-crossing reacting material (AO-CRM) even though only one is the structural gene for AO (Aldoxn). The other three genes (cin1, lxd and mal) coordinately "control" the levels of activity of AO and two related enzymes, xanthine dehydrogenase (XDH) and pyridoxal oxidase (PO). Contrary to their effects on AO-CRM, neither of these three mutants eliminate XDH-CRM. A model of interaction of these enzymes and genes controlling their activities is discussed.

THE DEVELOPMENTAL ANALYSIS OF ALDEHYDE OXIDASE ACTIVITY IN CIN ALLELIC HETEROZYGOTES OF DROSOPHILA MELANOGASTER

1982 ◽  
Vol 24 (1) ◽  
pp. 1-9 ◽  
Author(s):  
M. M. Bentley ◽  
J. H. Williamson
Keyword(s):  
Drosophila Melanogaster ◽  
Oxidase Activity ◽  
Developmental Stages ◽  
Aldehyde Oxidase ◽  
Activity Levels ◽  
Developmental Analysis

Aldehyde oxidase (AO) activity has been determined at 11 stages during the development of selected cin allelic homo-, hemi- and heterozygotes in Drosophila melanogaster. The AO activity levels found during development were completely consistent with the levels previously reported for adults, less than 24 h of age (Bentley and Williamson, 1979b). All of the cin homo- and hemizygotes tested exhibited no significant levels of AO activity at any of the 11 stages during development. All cin allelic heterozygotes, which were defined as complementing in adults, less than 24 h of age, displayed similar levels of complementation at all stages tested. Conversely, all cin allelic heterozygotes which were defined as noncomplementing in adults, less than 24 h of age, were found to lack measurable AO activity at all developmental stages tested.

Cynomolgus monkey liver aldehyde oxidase: extremely high oxidase activity and an attempt at purification

2000 ◽  
Vol 126 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Kazumi Sugihara ◽  
Yasuko Katsuma ◽  
Shigeyuki Kitamura ◽  
Shigeru Ohta ◽  
Morioki Fujitani ◽  
...  
Keyword(s):  
Cynomolgus Monkey ◽  
Oxidase Activity ◽  
Aldehyde Oxidase ◽  
Monkey Liver

Interspecific hybridization between Drosophila species group melanogaster sibling species: isozymic patterns and reproductive relationships

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 146-154 ◽  
Author(s):  
G. N. Goulielmos ◽  
S. N. Alahiotis
Keyword(s):  
Drosophila Melanogaster ◽  
Sex Ratio ◽  
Interspecific Hybrids ◽  
Aldehyde Oxidase ◽  
Species Group ◽  
Fitness Components ◽  
Phosphogluconate Dehydrogenase ◽  
Evolutionary Changes ◽  
Fertile Hybrids ◽  
Male Genital

In spite of previous consensus that no F1 fertile hybrids (of both sexes) could be produced between any mating combination of Drosophila melanogaster, D. simulans, and D. mauritiana, the present data indicate that such hybrids were obtained. Thus, some crosses between D. mauritiana females and D. simulans or D. melanogaster males yield F1 fertile hybrids (of both sexes) which have been named Masi (or Masi-2 and Masi-3) and Mame, respectively. Electrophoretic studies, using the species-diagnostic genes for 6-phosphogluconate dehydrogenase, alcohol dehydrogenase, and aldehyde oxidase (6-Pgd, Adh, and Aldox, respectively), were used to investigate the hybrid status, taking into consideration (i) their reproductive relationships, (ii) the coexistence of electromorphs from different species in the same hybrid, within the same generation, and (iii) the expression of the above electromorphs in the hybrids as well as in progeny from backcrosses, where unexpected irregularities and abnormalities were observed. These interspecific hybrids have been kept in our laboratory (as stocks) for 50 generations, to date, and have also been tested for various characteristics that contributed to the verification of their hybrid status (mating abilities, enzyme activities, hybrid sex ratio, the morphology of male genital arches and other fitness components). The finding of major genetic phenomena (e.g., allozymic repression) in these hybrid genomes gives some idea of the nature of events that could be associated with strong evolutionary changes, thus controlling speciation processes.Key words: Drosophila, electrophoresis, electromorphs, interspecific hybrids.

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