Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum

1987 ◽  
Vol 73 (3) ◽  
pp. 395-402 ◽  
Author(s):  
M. K. Ehlenfeldt ◽  
J. P. Helgeson
Keyword(s):  
Somatic Hybrids ◽  
Solanum Brevidens

Formation of parental-type and novel glycoalkaloids in somatic hybrids between Solanum brevidens and S. tuberosum

Plant Science ◽  
1996 ◽  
Vol 118 (2) ◽  
pp. 145-155 ◽  
Author(s):  
J. Laurila ◽  
I. Laakso ◽  
J.P.T. Valkonen ◽  
R. Hiltunen ◽  
E. Pehu
Keyword(s):  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Parental Type

scholarly journals Segregation and Recombination of Solanum brevidens Synteny Groups in Progeny of Somatic Hybrids With S. tuberosum: Intragenomic Equals or Exceeds Intergenomic Recombination

Genetics ◽  
1996 ◽  
Vol 142 (4) ◽  
pp. 1335-1348
Author(s):  
J Mitchell McGrath ◽  
Susan M Wielgus ◽  
John P Helgeson
Keyword(s):  
Somatic Hybrid ◽  
Rapd Markers ◽  
Somatic Hybrids ◽  
Specific Marker ◽  
Solanum Brevidens ◽  
Chromosome 5 ◽  
Backcross Population ◽  
Synteny Group ◽  
Potato Genome ◽  
Single Marker

Abstract The Solanum brmidens genome (2n = 2x = 24) was examined with randomly applied polymorphic DNA (RAPD) markers in a second backcross population derived from a S. brevidens + S. tuberosum somatic hybrid. RAPD markers cosegregated into 12 different S. brevidens synteny groups. Most synteny groups were nonrecombinant. However, nearly 40% of the S. brevidens synteny groups detected in this population were recombinant deletions that carried at least one, but not all, synteny group-specific RAPD markers. All S. brevidens synteny groups (except chromosome 5) were involved in recombination, and recombination occurred within most intervals between markers. About 20% of the recombinant S. brevidens synteny groups involved a single synteny group-specific marker. The inheritance of some single-marker representatives was followed in four BC3 families. At least nine changes in S. brevidens synteny groups had occurred during backcrossing. Six of the nine changes involved translocation of S. brevidens markers between nonhomologous S. brevidens chromosomes, and three S. brevidens markers may have been introgressed into the potato genome.

scholarly journals RFLP analysis of chromosomal segregation in progeny from an interspecific hexaploid somatic hybrid between Solanum brevidens and Solanum tuberosum.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1167-1173 ◽  
Author(s):  
C E Williams ◽  
S M Wielgus ◽  
G T Haberlach ◽  
C Guenther ◽  
H Kim-Lee ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Potato Cultivar ◽  
Rflp Analysis ◽  
Rflp Markers ◽  
Chromosome 2 ◽  
Solanum Brevidens ◽  
Homologous Pairing ◽  
Homeologous Recombination

Abstract Segregation of restriction fragment length polymorphism (RFLP) loci was monitored to determine the degree of homeologous pairing and recombination in a hexaploid somatic hybrid, A206, the result of protoplast fusion between Solanum tuberosum (PI 203900, a tetraploid cultivated potato) and Solanum brevidens (PI 218228), a diploid, sexually incompatible, distant relative harboring several traits for disease resistance. Somatic hybrid A206 was crossed to Katahdin, a tetraploid potato cultivar, to generate a segregating population of pentaploid progeny. Although the clones of the tetraploid S. tuberosum lines PI 203900 and Katahdin were highly polymorphic, the diploid S. brevidens clone was homozygous at all but two of the tested RFLP loci. Thus, homeologous recombination could be detected only when S. tuberosum and S. brevidens chromosomes paired and the S. brevidens homologs then segregated into separate gametes. A bias toward homologous pairing was observed for all 12 chromosomes. At least four and perhaps six chromosomes participated in homologous pairing only; each of 24 progeny contained all S. brevidens-derived RFLP markers for chromosomes 4, 8, 9 and 10. The remaining six chromosomes paired with their homolog(s) about twice as often as expected if hexaploid pairings were completely random. Where detectable with RFLPs, homeologous recombination (both single and double) occurred at a frequency of 1.31 per chromosome. Cytological observations of meiosis I in the somatic hybrid indicated that homeologous pairing had occurred. Enhanced recombinational activity was observed for chromosome 2. A specific small deletion from chromosome 4 was detected in A206 and 11 other somatic hybrids out of 14 screened.(ABSTRACT TRUNCATED AT 250 WORDS)

Isolation and characterisation of somatic hybrids of diploid Solanum tuberosum and Solanum brevidens and the use of amylose-free starch mutation for detection of introgression

Euphytica ◽  
1993 ◽  
Vol 69 (3) ◽  
pp. 191-201 ◽  
Author(s):  
E. Jacobsen ◽  
R. Malvar ◽  
D. J. Huigen ◽  
J. E. M. Bergervoet ◽  
M. S. Ramanna
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Amylose Free

scholarly journals Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences

1998 ◽  
Vol 7 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Nora L. V. Lapitan ◽  
Dennis L. Knudson ◽  
Eija Pehu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Digital Imaging ◽  
Somatic Hybrid ◽  
Fluorescent Dyes ◽  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Specific Sequences

Two Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin-14-dATP and they were detected with reporter molecules conjugated to fluorescent dyes using digital imaging. The tandemly repeated sequences hybridized mostly near the telomeres of the chromosomes of S. brevidens. Using these two probes, it was possible to identify chromosomes containing repetitive DNA of S. brevidens both in the somatic hybrids between S. brevidens and S. tuberosum, and somatohaploids derived from the somatic hybrids. These cytological analyses showed that for the largest part genomes of the hexaploid somatic hybrids and their anther-derived triploid somatohaploids were composed of the genome of S. brevidens.

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