Isolation and characterisation of somatic hybrids of diploid Solanum tuberosum and Solanum brevidens and the use of amylose-free starch mutation for detection of introgression

Euphytica ◽  
1993 ◽  
Vol 69 (3) ◽  
pp. 191-201 ◽  
Author(s):  
E. Jacobsen ◽  
R. Malvar ◽  
D. J. Huigen ◽  
J. E. M. Bergervoet ◽  
M. S. Ramanna
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Amylose Free

scholarly journals RFLP analysis of chromosomal segregation in progeny from an interspecific hexaploid somatic hybrid between Solanum brevidens and Solanum tuberosum.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1167-1173 ◽  
Author(s):  
C E Williams ◽  
S M Wielgus ◽  
G T Haberlach ◽  
C Guenther ◽  
H Kim-Lee ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Potato Cultivar ◽  
Rflp Analysis ◽  
Rflp Markers ◽  
Chromosome 2 ◽  
Solanum Brevidens ◽  
Homologous Pairing ◽  
Homeologous Recombination

Abstract Segregation of restriction fragment length polymorphism (RFLP) loci was monitored to determine the degree of homeologous pairing and recombination in a hexaploid somatic hybrid, A206, the result of protoplast fusion between Solanum tuberosum (PI 203900, a tetraploid cultivated potato) and Solanum brevidens (PI 218228), a diploid, sexually incompatible, distant relative harboring several traits for disease resistance. Somatic hybrid A206 was crossed to Katahdin, a tetraploid potato cultivar, to generate a segregating population of pentaploid progeny. Although the clones of the tetraploid S. tuberosum lines PI 203900 and Katahdin were highly polymorphic, the diploid S. brevidens clone was homozygous at all but two of the tested RFLP loci. Thus, homeologous recombination could be detected only when S. tuberosum and S. brevidens chromosomes paired and the S. brevidens homologs then segregated into separate gametes. A bias toward homologous pairing was observed for all 12 chromosomes. At least four and perhaps six chromosomes participated in homologous pairing only; each of 24 progeny contained all S. brevidens-derived RFLP markers for chromosomes 4, 8, 9 and 10. The remaining six chromosomes paired with their homolog(s) about twice as often as expected if hexaploid pairings were completely random. Where detectable with RFLPs, homeologous recombination (both single and double) occurred at a frequency of 1.31 per chromosome. Cytological observations of meiosis I in the somatic hybrid indicated that homeologous pairing had occurred. Enhanced recombinational activity was observed for chromosome 2. A specific small deletion from chromosome 4 was detected in A206 and 11 other somatic hybrids out of 14 screened.(ABSTRACT TRUNCATED AT 250 WORDS)

Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens

1986 ◽  
Vol 72 (6) ◽  
pp. 787-793 ◽  
Author(s):  
R. J. Kemble ◽  
T. L. Barsby ◽  
R. S. C. Wong ◽  
J. F. Shepard
Keyword(s):  
Mitochondrial Dna ◽  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Dna Rearrangements

Identification of mitotic chromosomes of tuberous and non-tuberous Solanum species (Solanum tuberosum and Solanum brevidens) by GISH in their interspecific hybrids

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 442-449 ◽  
Author(s):  
T Gavrilenko ◽  
J Larkka ◽  
E Pehu ◽  
V -M Rokka
Keyword(s):  
Solanum Tuberosum ◽  
Second Generation ◽  
Somatic Hybridization ◽  
Somatic Hybrids ◽  
Solanum Tuberosum L ◽  
Solanum Species ◽  
Solanum Brevidens ◽  
Mitotic Chromosomes ◽  
Aa Genome

GISH (genomic in situ hybridization) was applied for the analysis of mitotic chromosome constitutions of somatic hybrids and their derivatives between dihaploid clones of cultivated potato (Solanum tuberosum L.) (2n = 2x = 24, AA genome) and the diploid, non-tuberous, wild species Solanum brevidens Phil. (2n = 2x = 24, EE genome). Of the primary somatic hybrids, both tetraploid (2n = 4x) and hexaploid (2n = 6x) plants were found with the genomic constitutions of AAEE and AAEEEE, respectively. Androgenic haploids (somatohaploids) derived from the tetraploid somatic hybrids had the genomic constitutions of AE (2n = 2x = 24) and haploids originating from the hexaploid hybrids were triploid AEE (2n = 3x = 33 and 2n = 3x = 36). As a result of subsequent somatic hybridization from a fusion between dihaploid S. tuberosum (2n = 2x = 24, genome AA) and a triploid somatohaploid (2n = 3x = 33, genome AEE), second-generation somatic hybrids were obtained. These somatic hybrids were pentaploids (2n = 5x, genome AAAEE), but had variable chromosome numbers. GISH analysis revealed that both primary and second-generation somatic hybrids had lost more chromosomes of S. brevidens than of S. tuberosum.Key words: anther culture, genome, haploid, potato, somatic hybridization.

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

2005 ◽  
Vol 83 (1) ◽  
pp. 1-11 ◽  
Author(s):  
S. Trabelsi ◽  
R. Gargouri-Bouzid ◽  
F. Vedel ◽  
A. Nato ◽  
L. Lakhoua ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Solanum Vernei
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