Toxoplasma gondii in tissue culture and maintenance at low temperatures

1968 ◽  
Vol 30 (4) ◽  
Author(s):  
Paola Franchi ◽  
ErichE.A. Hahn
Keyword(s):  
Tissue Culture ◽  
Toxoplasma Gondii ◽  
Low Temperatures

Effect of Freezing on Infectivity of Toxoplasma Gondii Tissue Cysts in Pork

1991 ◽  
Vol 54 (9) ◽  
pp. 687-690 ◽  
Author(s):  
A. W. KOTULA ◽  
J. P. DUBEY ◽  
A. K. SHARAR ◽  
C. D. ANDREWS ◽  
S. K. SHEN ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Linear Regression ◽  
Regression Model ◽  
Least Squares ◽  
Infected Mouse ◽  
Low Temperatures ◽  
Trichinella Spiralis ◽  
Square Root ◽  
Uniform Thickness

To study the effect of low temperatures on infectivity of Toxoplasma gondii tissue cysts, pork from infected pigs was mixed with infected mouse brains and homogenized thoroughly. Twenty-gram samples of infected homogenized meat which were sealed in plastic pouches, pressed to a uniform thickness resulting in samples having the dimensions of ~.2 × 16 × 18 cm, were subjected to temperatures of −1 to −171.1 °C for 1 s to 67.2 d. Treated samples were digested in HCl-pepsin solution and infectivity assayed in mice. A regression model from these data is described by the least squares linear regression: Square root of time for the inactivation of T. gondii (h) = 26.72 + 2.16 temperature (°C) with r = 0.77. T. gondii tissue cysts remained viable usually up to 22.4 d at −1 and −3.9°C and 11.2 d at −6.7°C but were usually rendered nonviable by freezing at −12.37°C. These data demonstrate that T. gondii tissue cysts are inactivated by freezing more readily than encysted Trichinella spiralis larvae.

Freeze Preservation of Tissue Culture Propagated Toxoplasma gondii

1974 ◽  
Vol 60 (2) ◽  
pp. 368 ◽  
Author(s):  
Robert O. Bollinger ◽  
Nabil Musallam ◽  
Cyril S. Stulberg
Keyword(s):  
Tissue Culture ◽  
Toxoplasma Gondii
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