scholarly journals Subpopulations of antibodies directed against evolutionarily conserved regions of the insulin molecule in insulin-treated patients

Diabetologia ◽  
1982 ◽  
Vol 23 (6) ◽  
Author(s):  
F. Karlsson ◽  
L.C. Harrison ◽  
C.R. Kahn ◽  
A. Itin ◽  
J. Roth
Keyword(s):  
Conserved Regions ◽  
Insulin Molecule ◽  
Evolutionarily Conserved

scholarly journals NLIP and HAD-like Domains of Pah1 and Lipin 1 Phosphatidate Phosphatases Are Essential for Their Catalytic Activities

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5470
Author(s):  
Wei-Hsin Hsu ◽  
Yi-Hao Huang ◽  
Pin-Ru Chen ◽  
Lu-Sheng Hsieh
Keyword(s):  
Catalytic Activity ◽  
Intrinsically Disordered Proteins ◽  
Protein Solubility ◽  
Disordered Proteins ◽  
Haloacid Dehalogenase ◽  
Catalytic Activities ◽  
Intrinsically Disordered ◽  
Conserved Regions ◽  
Evolutionarily Conserved ◽  
Lipin 1

Saccharomyces cerevisiae Pah1 phosphatidate phosphatase (PAP) catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol, controlling phospholipids and triacylglycerol metabolisms. Pah1 and human Lipin 1 are intrinsically disordered proteins with 56% and 43% unfolded regions, respectively. Truncation analysis of the conserved and non-conserved regions showed that N- and C-conserved regions are essential for the catalytic activity of Pah1. PAP activities can be detected in the conserved N-terminal Lipin (NLIP) domain and C-terminal Lipin (CLIP)/haloacid dehalogenase (HAD)-like domain of Pah1 and Lipin 1, suggesting that the evolutionarily conserved domains are essential for the catalytic activity. The removal of disordered hydrophilic regions drastically reduced the protein solubility of Pah1. Thioredoxin is an efficient fusion protein for production of soluble NLIP–HAD recombinant proteins in Escherichia coli.

scholarly journals Molecular genetic aetiology of general cognitive function is enriched in evolutionarily conserved regions

2016 ◽  
Vol 6 (12) ◽  
pp. e980-e980 ◽  
Author(s):  
W D Hill ◽  
◽  
G Davies ◽  
S E Harris ◽  
S P Hagenaars ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Molecular Genetic ◽  
Conserved Regions ◽  
Evolutionarily Conserved

scholarly journals Genetic and Functional Interaction of Evolutionarily Conserved Regions of the Prp18 Protein and the U5 snRNA

2005 ◽  
Vol 25 (6) ◽  
pp. 2107-2116 ◽  
Author(s):  
Dagmar Bačíková ◽  
David S. Horowitz
Keyword(s):  
Functional Relationship ◽  
Mrna Splicing ◽  
Second Step ◽  
Functional Interaction ◽  
Synthetic Lethal ◽  
Conserved Regions ◽  
Evolutionarily Conserved ◽  
Conserved Region ◽  
Relationship Of ◽  
U5 Snrna

ABSTRACT Both the Prp18 protein and the U5 snRNA function in the second step of pre-mRNA splicing. We identified suppressors of mutant prp18 alleles in the gene for the U5 snRNA (SNR7). The suppressors' U5 snRNAs have either a U4-to-A or an A8-to-C mutation in the evolutionarily invariant loop 1 of U5. Suppression is specific for prp18 alleles that encode proteins with mutations in a highly conserved region of Prp18 which forms an unstructured loop in crystals of Prp18. The snr7 suppressors partly restored the pre-mRNA splicing activity that was lost in the prp18 mutants. The close functional relationship of Prp18 and U5 is emphasized by the finding that two snr7 alleles, U5A and U6A, are dominant synthetic lethal with prp18 alleles. Our results support the idea that Prp18 and the U5 snRNA act in concert during the second step of pre-mRNA splicing and suggest a model in which the conserved loop of Prp18 acts to stabilize the interaction of loop 1 of the U5 snRNA with the splicing intermediates.

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