The role of trehalose as a substitute for nitrogen-containing compatible solutes (Ectothiorhodospira halochloris)

1990 ◽  
Vol 153 (6) ◽  
pp. 607-613 ◽  
Author(s):  
Erwin A. Galinski ◽  
Ruth M. Herzog
Keyword(s):  
Compatible Solutes ◽  
Ectothiorhodospira Halochloris

Evolution of the diverse biological roles of inositols

2007 ◽  
Vol 74 ◽  
pp. 223-246 ◽  
Author(s):  
Robert H. Michell
Keyword(s):  
Membrane Trafficking ◽  
Cell Signalling ◽  
Compatible Solutes ◽  
Mirror Image ◽  
Membrane Phospholipids ◽  
Functional Diversification ◽  
Redox Reagent ◽  
Ca2 Channels ◽  
Mycobacterium Spp

Several of the nine hexahydroxycylohexanes (inositols) have functions in Biology, with myo-inositol (Ins) in most of the starring roles; and Ins polyphosphates are amongst the most abundant organic phosphate constituents on Earth. Many Archaea make Ins and use it as a component of diphytanyl membrane phospholipids and the thermoprotective solute di-L-Ins-1,1′-phosphate. Few bacteria make Ins or use it, other than as a carbon source. Those that do include hyperthermophilic Thermotogales (which also employ di-l-Ins-1,1′-phosphate) and actinomycetes such as Mycobacterium spp. (which use mycothiol, an inositol-containing thiol, as an intracellular redox reagent and have characteristic phosphatidylinositol-linked surface oligosaccharides). Bacteria acquired their Ins3P synthases by lateral gene transfer from Archaea. Many eukaryotes, including stressed plants, insects, deep-sea animals and kidney tubule cells, adapt to environmental variation by making or accumulating diverse inositol derivatives as ‘compatible’ solutes. Eukaryotes use phosphatidylinositol derivatives for numerous roles in cell signalling and regulation and in protein anchoring at the cell surface. Remarkably, the diradylglycerol cores of archaeal and eukaryote/bacterial glycerophospholipids have mirror image configurations: sn-2,3 and sn-1,2 respectively. Multicellular animals and amoebozoans exhibit the greatest variety of functions for PtdIns derivatives, including the use of PtdIns(3,4,5)P3 as a signal. Evolutionarily, it seems likely that (i) early archaeons first made myo-inositol approx. 3500 Ma (million years) ago; (ii) archeons brought inositol derivatives into early eukaryotes (approx. 2000 Ma?); (iii) soon thereafter, eukaryotes established ubiquitous functions for phosphoinositides in membrane trafficking and Ins polyphosphate synthesis; and (iv) since approx. 1000 Ma, further waves of functional diversification in amoebozoans and metazoans have introduced Ins(1,4,5)P3 receptor Ca2+ channels and the messenger role of PtdIns(3,4,5)P3.

Major Compatible Solutes and Structural Adaptation of Proteins in Extremophiles

2022 ◽  
pp. 161-186
Author(s):  
Hardik Shah ◽  
Khushbu Panchal ◽  
Amisha Panchal
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Osmotic Stress ◽  
Amino Acid Composition ◽  
Scientific Community ◽  
Compatible Solutes ◽  
Organic Solutes ◽  
Structural Adaptation ◽  
Extreme Habitats

Extremophiles are the most ancient microbes on the Earth and also a center of attraction for the scientific community for research because of their ability to adapt to extreme habitats. Compatible solutes are among those factors which enable these microorganisms to thrive in such extreme habitats. Under osmotic stress, the majority of extremophiles accumulate specific organic solutes such as amino acids, sugars, polyols, and their derivatives. In addition, proteins in extremophiles are found to be evolved by changing their amino acid composition to alter the hydrophobicity of its core and surface charge to maintain activity. This chapter encompasses a comprehensive study about the role of various compatible solutes in the endurance of microorganisms under extremophilic conditions, synthesis of compatible solutes, nature of extremophilic proteins, and their applications. Furthermore, an attempt has been made to cover various strategies adopted by the scientific community while pursuing research on compatible solutes.

scholarly journals Role of σB in Regulating the Compatible Solute Uptake Systems of Listeria monocytogenes: Osmotic Induction of opuC Is σB Dependent

2003 ◽  
Vol 69 (4) ◽  
pp. 2015-2022 ◽  
Author(s):  
Katy R. Fraser ◽  
David Sue ◽  
Martin Wiedmann ◽  
Kathryn Boor ◽  
Conor P. O'Byrne
Keyword(s):  
Listeria Monocytogenes ◽  
Solute Transport ◽  
Compatible Solutes ◽  
Strain Analysis ◽  
Compatible Solute ◽  
Transport Systems ◽  
Carnitine Transporter ◽  
Solute Uptake ◽  
Carnitine Transport

ABSTRACT The regulation of the compatible solute transport systems in Listeria monocytogenes by the stress-inducible sigma factor σB was investigated. Using wild-type strain 10403S and an otherwise isogenic strain carrying an in-frame deletion in sigB, we have examined the role of σB in regulating the ability of cells to utilize betaine and carnitine during growth under conditions of hyperosmotic stress. Cells lacking σB were defective for the utilization of carnitine but retained the ability to utilize betaine as an osmoprotectant. When compatible solute transport studies were performed, the initial rates of uptake of both betaine and carnitine were found to be reduced in the sigB mutant; carnitine transport was almost abolished, whereas betaine transport was reduced to approximately 50% of that of the parent strain. Analysis of the cytoplasmic pools of compatible solutes during balanced growth revealed that both carnitine and betaine steady-state pools were reduced in the sigB mutant. Transcriptional reporter fusions to the opuC (which encodes an ABC carnitine transporter) and betL (which encodes an a secondary betaine transporter) operons were generated by using a promoterless copy of the gus gene from Escherichia coli. Measurement of β-glucuronidase activities directed by opuC-gus and betL-gus revealed that transcription of opuC is largely σB dependent, consistent with the existence of a potential σB consensus promoter motif upstream from opuCA. The transcription of betL was found to be sigB independent. Reverse transcriptase PCR experiments confirmed these data and indicated that the transcription of all three known compatible solute uptake systems (opuC, betL, and gbu), as well as a gene that is predicted to encode a compatible solute transporter subunit (lmo1421) is induced in response to elevated osmolarity. The osmotic induction of opuCA and lmo1421 was found to be strongly σB dependent. Together these observations suggest that σB plays a major role in the regulation of carnitine utilization by L. monocytogenes but is not essential for betaine utilization by this pathogen.

scholarly journals Role of Polyamines in Regulating Abiotic Stress Tolerance in Agricultural Crops: A Review

2021 ◽  
Author(s):  
G. Lakshmi ◽  
R. Beena
Keyword(s):  
Abiotic Stress ◽  
Review Paper ◽  
Abiotic Stress Tolerance ◽  
Compatible Solutes ◽  
Acid Synthesis ◽  
Climatic Fluctuations ◽  
Over Expression ◽  
Cellular Processes ◽  
Micro Rnas

At present plants had to face adverse climatic fluctuations which are often detrimental for their growth in order to cope up with this situation, they develop certain adaptive tactics like synthesis of new proteins, micro RNAs, compatible solutes/ osmolites and radical release to avoid its adverse effects. Of these study on compatible solutes such as polyamines (PAs) gained popularity among researchers. In plant, they are involved in a wide variety of regulatory and cellular processes under normal conditions. During these stresses they acts by activating biosynthesis of signaling molecules like NO, H2O2; affects abscisic acid synthesis; Ca2+ homeostasis; ion channel signaling or even apoptosis in severe conditions. Over expression of PAs is reported in various stresses due to the presence of stress-responsive elements in the promoters of PA biosynthetic genes. This review paper, summarizes the effect of polyamines in boosting plants growth during abiotic stress and its mechanisms of action.

scholarly journals The physiology of salinity tolerance in larvae of two species of Culex mosquitoes: the role of compatible solutes

2000 ◽  
Vol 203 (4) ◽  
pp. 821-830 ◽  
Author(s):  
M.L. Patrick ◽  
T.J. Bradley
Keyword(s):  
Salinity Tolerance ◽  
Sea Water ◽  
Compatible Solutes ◽  
Compatible Solute ◽  
Ion Regulation ◽  
Physiological Basis ◽  
Environmental Salinity ◽  
Acute Responses ◽  
Nacl Concentration

We investigated the physiological basis for differences in salinity tolerance ranges in mosquito larvae of the genus Culex. We examined the response of larvae of C. quinquefasciatus, a freshwater obligate, and C. tarsalis, a euryhaline osmoconformer, following transfer from fresh water to 34% sea water. Hemolymph Na(+) and Cl(−) levels increased similarly in both species, indicating that ion regulation does not differ under these conditions. C. quinquefasciatus responded to increased environmental salinity with increased hemolymph levels of serine, but suffered a significant reduction in levels of trehalose. C. tarsalis responded to increased environmental salinity with increased hemolymph levels of both proline and trehalose. When C. tarsalis larvae were held in 64% sea water, which C. quinquefasciatus larvae cannot tolerate, hemolymph proline and trehalose were accumulated approximately 50-fold and twofold, respectively, relative to freshwater values. We found that proline serves as both an intra- and extracellular compatible solute in C. tarsalis, the first such circumstance documented in an animal in response to increased environmental salinity. Analyses of the acute responses of the two species to an increase in salinity (from 30% to 50% sea water) indicate that larvae of C. tarsalis are able to volume-regulate via drinking and to attenuate increases in hemolymph NaCl concentration using unknown mechanisms during large, rapid increases in salinity.

Role of Compatible Solutes in Enhancing Antioxidative Defense in Plants Exposed to Metal Toxicity

2018 ◽  
pp. 207-228 ◽  
Author(s):  
Neha Handa ◽  
Sukhmeen Kaur Kohli ◽  
Ravdeep Kaur ◽  
Anket Sharma ◽  
Vinod Kumar ◽  
...  
Keyword(s):  
Metal Toxicity ◽  
Compatible Solutes ◽  
Antioxidative Defense
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