Behavioural effects of human fetal dopamine neurons grafted in a rat model of Parkinson's disease

1986 ◽  
Vol 65 (1) ◽  
Author(s):  
P. Brundin ◽  
O.G. Nilsson ◽  
R.E. Strecker ◽  
O. Lindvall ◽  
B. �stedt ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Rat Model ◽  
Dopamine Neurons ◽  
Behavioural Effects

scholarly journals Human ESC-Derived Dopamine Neurons Show Similar Preclinical Efficacy and Potency to Fetal Neurons when Grafted in a Rat Model of Parkinson’s Disease

2014 ◽  
Vol 15 (5) ◽  
pp. 653-665 ◽  
Author(s):  
Shane Grealish ◽  
Elsa Diguet ◽  
Agnete Kirkeby ◽  
Bengt Mattsson ◽  
Andreas Heuer ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Rat Model ◽  
Dopamine Neurons ◽  
Preclinical Efficacy

Interference with anoikis-induced cell death of dopamine neurons: Implications for augmenting embryonic graft survival in a rat model of Parkinson's disease

2003 ◽  
Vol 464 (2) ◽  
pp. 172-179 ◽  
Author(s):  
Deanna M. Marchionini ◽  
Timothy J. Collier ◽  
Maria Camargo ◽  
Susan McGuire ◽  
Mark Pitzer ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cell Death ◽  
Graft Survival ◽  
Rat Model ◽  
Dopamine Neurons

scholarly journals Congrong Shujing Granule-Induced GRP78 Expression Reduced Endoplasmic Reticulum Stress and Neuronal Apoptosis in the Midbrain in a Parkinson’s Disease Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Qian Xu ◽  
Shasha Yang ◽  
Fangzhen Wu ◽  
Yao Lin ◽  
Jianan Zhong ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Rat Model ◽  
Neuronal Apoptosis ◽  
Dopamine Neurons ◽  
High Dose ◽  
Model Group ◽  
Marker Proteins

The main pathological changes inherent in Parkinson’s disease (PD) are degeneration and loss of dopamine neurons in the midbrain and formation of Lewy bodies. Many studies have shown that the pathogenesis of PD is closely related to endoplasmic reticulum (ER) oxidative stress. This study combined various traditional Chinese medicines to prepare Congrong Shujing granules (CSGs). The optimal dose combination of the ingredients was identified by experimental intervention in SH-SY5Y cells in vitro. A PD rat model was established by intraperitoneal injection of rotenone sunflower oil emulsion. The suspension tests were performed on the 14th day after modeling and also on the 14th day after CSG intervention (5.88 g/kg, 11.76 g/kg, and 23.52 g/kg). We evaluated the changes in motor function and the expression of neuronal cell functional marker proteins, ER stress (ERS) marker proteins, and apoptosis-related pathway proteins of neuronal cells. Changes in cellular ultrastructure were observed by electron microscopy. Our results showed that CSG treatment lengthened the duration of PD rats’ gripping to the wire. 78 kDa glucose-regulated protein (GRP78) expression in the substantia nigra was significantly upregulated in the middle- and high-dose CSG groups after 14 days of treatment compared with the model group. The expression of the key dopaminergic neuron functional enzyme tyrosine hydroxylase (TH) and cerebral dopamine neurotrophic factor (CDNF) was elevated. The expression of c-Jun N-terminal kinase (JNK) and phosphorylated c-Jun decreased, and cell apoptosis was significantly reduced. Compared with the model group, the treatment groups had fewer ER fragmentation and degranulation (ribosome shedding) and abundant ER and mitochondria suggesting that CSG reduced ER stress and neuronal apoptosis in the midbrain of a PD rat model by inducing the expression of molecular chaperone GRP78.

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