?-adrenergic receptor internalization and processing: role of transglutaminase and lysosomes

AbstractUterine inflammation is a very common and serious condition in domestic animals. To development and progression of this pathology often lead disturbances in myometrial contractility. Participation of β1-, β2- and β3-adrenergic receptors (ARs) in noradrenaline (NA)-influenced contractility of the pig inflamed uterus was studied. The gilts of SAL- and E.coli-treated groups were administered saline or E.coli suspension into the uterine horns, respectively. Laparotomy was only done in the CON group. Compared to the period before NA administration, this neurotransmitter reduced the tension, amplitude and frequency in uterine strips of the CON and SAL groups. In the E.coli group, NA decreased the amplitude and frequency, and these parameters were lower than in other groups. In the CON, SAL and E.coli groups, β1- and β3-ARs antagonists in more cases did not significantly change and partly eliminated NA inhibitory effect on amplitude and frequency, as compared to NA action alone. In turn, β2-ARs antagonist completely abolished NA relaxatory effect on these parameters in three groups. Summarizing, NA decreases the contractile amplitude and frequency of pig inflamed uterus via all β-ARs subtypes, however, β2-ARs have the greatest importance. Given this, pharmacological modulation of particular β-ARs subtypes can be used to increase inflamed uterus contractility.

Download Full-text

Relationship between an altered membrane form and a low affinity form of the beta-adrenergic receptor occurring during catecholamine-induced desensitization. Evidence for receptor internalization.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)71288-3 ◽

1984 ◽

Vol 259 (19) ◽

pp. 11844-11850 ◽

Cited By ~ 1

Author(s):

M L Toews ◽

G L Waldo ◽

T K Harden ◽

J P Perkins

Keyword(s):

Adrenergic Receptor ◽

Receptor Internalization ◽

Beta Adrenergic Receptor ◽

Beta Adrenergic

Download Full-text

Cardioprotective role of chunglijagam-tang, a Korean herbal medicine, against β-adrenergic receptor stimulation-induced myocardial toxicity

Advances in Integrative Medicine ◽

10.1016/j.aimed.2019.03.404 ◽

2019 ◽

Vol 6 ◽

pp. S138-S139

Author(s):

Ho-Sung Lee ◽

Seung Hoon Lee ◽

Dal-Seok Oh

Keyword(s):

Herbal Medicine ◽

Adrenergic Receptor ◽

Receptor Stimulation

Download Full-text

Loss of M2 muscarinic receptor function inhibits development of hypoxic bradycardia and alters cardiac β-adrenergic sensitivity in larval zebrafish (Danio rerio)

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00036.2009 ◽

2009 ◽

Vol 297 (2) ◽

pp. R412-R420 ◽

Cited By ~ 17

Author(s):

Shelby L. Steele ◽

Kwok Hong Andy Lo ◽

Vincent Wai Tsun Li ◽

Shuk Han Cheng ◽

Marc Ekker ◽

...

Keyword(s):

Heart Rate ◽

Muscarinic Receptor ◽

Danio Rerio ◽

Antisense Oligonucleotides ◽

Adrenergic Receptor ◽

Larval Zebrafish ◽

M2 Muscarinic Receptor ◽

Β Adrenergic Receptors ◽

Relative Mrna Expression

Fish exposed to hypoxia develop decreased heart rate, or bradycardia, the physiological significance of which remains unknown. The general muscarinic receptor antagonist atropine abolishes the development of this hypoxic bradycardia, suggesting the involvement of muscarinic receptors. In this study, we tested the hypothesis that the hypoxic bradycardia is mediated specifically by stimulation of the M2 muscarinic receptor, the most abundant subtype in the vertebrate heart. Zebrafish ( Danio rerio) were reared at two levels of hypoxia (30 and 40 Torr Po2) from the point of fertilization. In hypoxic fish, the heart rate was significantly lower than in normoxic controls from 2 to 10 days postfertilization (dpf). At the more severe level of hypoxia (30 Torr Po2), there were significant increases in the relative mRNA expression of M 2 and the cardiac type β-adrenergic receptors ( β1AR, β2aAR, and β2bAR) at 4 dpf. The hypoxic bradycardia was abolished (at 40 Torr Po2) or significantly attenuated (at 30 Torr Po2) in larvae experiencing M2 receptor knockdown (using morpholino antisense oligonucleotides). Sham-injected larvae exhibited typical hypoxic bradycardia in both hypoxic regimens. The expression of β1AR, β2aAR, β2bAR, and M 2 mRNA was altered at various stages between 1 and 4 dpf in larvae experiencing M2 receptor knockdown. Interestingly, M2 receptor knockdown revealed a cardioinhibitory role for the β2-adrenergic receptor. This is the first study to demonstrate a specific role of the M2 muscarinic receptor in the initiation of hypoxic bradycardia in fish.

Download Full-text