Another class of vicilin gene in Pisum

Planta ◽  
1990 ◽  
Vol 182 (1) ◽  
Author(s):  
C. Domoney ◽  
R. Casey
Keyword(s):  
Vicilin Gene

The Structure of a Vicia faba Vicilin Gene

1988 ◽  
Vol 183 (2-3) ◽  
pp. 233-242 ◽  
Author(s):  
Winfriede Weschke ◽  
Ronald Bassüner ◽  
Nong Van Hai ◽  
Andreas Czihal ◽  
Helmut Baümlein ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Vicilin Gene

Increased capacity for sucrose uptake leads to earlier onset of protein accumulation in developing pea seeds

2005 ◽  
Vol 32 (11) ◽  
pp. 997 ◽  
Author(s):  
Elke G. Rosche ◽  
Daniel Blackmore ◽  
Christina E. Offler ◽  
John W. Patrick
Keyword(s):  
Storage Protein ◽  
Protein Biosynthesis ◽  
Protein Bodies ◽  
Protein Accumulation ◽  
Sucrose Uptake ◽  
Wild Type ◽  
Protein Levels ◽  
Vicilin Gene ◽  
And Storage

Pea (Pisum sativum L.) cotyledons, overexpressing a potato sucrose transporter (StSUT1), were used to explore the hypothesis that sucrose stimulates the onset of storage protein biosynthesis. The study focused on the transition between pre-storage and storage phases of seed development. During this period supply of sucrose and hexose to transgenic cotyledons was unaffected by StSUT1 expression. However, protoplasmic levels of sucrose but not hexoses were elevated in transgenic cotyledons. Total protein levels in cotyledons followed the same temporal trend as observed for sucrose and this was reflected in an earlier appearance of protein bodies. Protein levels in wild type and StSUT1 cotyledons were found to lie on the same sucrose dose-response curve and this could be reproduced in vitro when wild type cotyledons were cultured on media containing various sucrose concentrations. Rates of [14C]sucrose uptake and incorporation into polymeric forms were consistent with protoplasmic sucrose supplying a proportion of the carbon skeletons required for storage protein accumulation. In addition, vicilin gene expression was up-regulated earlier in StSUT1 cotyledons. We conclude that sucrose functions both as a signal and fuel to stimulate storage protein accumulation and assembly into protein bodies. An earlier stimulation of storage protein synthesis is considered to largely account for the 14% increase in protein levels of StSUT1 seeds at harvest.

scholarly journals Regulation of storage-protein synthesis in pea (Pisum sativum L.) cotyledons under conditions of sulphur deficiency

1985 ◽  
Vol 232 (1) ◽  
pp. 261-265 ◽  
Author(s):  
I M Evans ◽  
J A Gatehouse ◽  
D Boulter
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Gene Transcription ◽  
Storage Protein ◽  
Control Of Gene Expression ◽  
Sulphur Deficiency ◽  
Pisum Sativum L ◽  
S Deficiency ◽  
Vicilin Gene ◽  
Storage Protein Genes

The effects of sulphur deficiency on the expression of storage-protein genes in developing pea (Pisum sativum) cotyledons were studied. Legumin-gene transcription was decreased by S-deficiency, but not to the same extent as the decrease in the level of legumin mRNA. Vicilin-gene transcription was not significantly affected. Control of gene expression may thus occur during transcription and/or post-transcriptional events.

Transient expression of a lysine-rich vicilin gene ofVicia faba in barley endosperm detected by immunological tissue printing after particle bombardment

1995 ◽  
Vol 15 (1-2) ◽  
pp. 125-128
Author(s):  
Ute Heim ◽  
Renate Manteuffel ◽  
Helmut Bäumlein ◽  
Hans-Henning Steinbiss ◽  
Ulrich Wobus
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Barley Endosperm ◽  
Tissue Printing ◽  
Ofvicia Faba ◽  
Vicilin Gene

scholarly journals The vicilin gene family of pea (Pisum sativumL.): a complete cDNA coding sequence for preprovicilin

1983 ◽  
Vol 11 (8) ◽  
pp. 2367-2380 ◽  
Author(s):  
Grantley W. Lycett ◽  
Ashton J. Delauney ◽  
John A. Gatehouse ◽  
John Gilroy ◽  
Ronald R.D. Croy ◽  
...  
Keyword(s):  
Gene Family ◽  
Coding Sequence ◽  
Vicilin Gene

The sequence of a pea vicilin gene and its expression in transgenic tobacco plants

1988 ◽  
Vol 11 (5) ◽  
pp. 683-695 ◽  
Author(s):  
Thomas J. V. Higgins ◽  
Edward J. Newbigin ◽  
Donald Spencer ◽  
Danny J. Llewellyn ◽  
Stuart Craig
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Vicilin Gene

scholarly journals Nucleotide sequence of a field bean (Vicia fabaL.var.minor) vicilin gene

1987 ◽  
Vol 15 (23) ◽  
pp. 10065-10065 ◽  
Author(s):  
Winfriede Weschke ◽  
Helmut Bäumlein ◽  
Ulrich Wobus
Keyword(s):  
Nucleotide Sequence ◽  
Field Bean ◽  
Vicilin Gene

Evidence that the N-terminal extension of the Vicieae convicilin genes evolved by intragenic duplications and trinucleotide expansions

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1022-1030 ◽  
Author(s):  
L E Sáenz de Miera ◽  
M Pérez de la Vega
Keyword(s):  
Amino Acids ◽  
Lens Culinaris ◽  
Storage Proteins ◽  
Phylogenetic Analyses ◽  
Single Gene ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Gene Families ◽  
Plant Seed ◽  
Vicilin Gene

This study was aimed to identify lentil (Lens culinaris subsp. culinaris) convicilin genes and to carry out a comparative analysis of these genes in the tribe Vicieae. Convicilins differ from vicilins, a related group of plant seed storage proteins, mainly by the presence of an additional sequence of amino acids in the sequence corresponding to the first exon, referred as the N-terminal extension. A single gene for convicilin, a component of legume seed storage proteins, was identified in the cultivated lentil. In this species, the N-terminal extension is formed by a stretch of 126 amino acids of which 59.2% are charged amino acids: 29.6% glutamic acid, 3.2% aspartic acid, 14.4% arginine, 8.8% lysine, and 3.2% histidine. This lentil convicilin sequence is similar to the sequence of convicilins in other species of the tribe Vicieae. However, the size of the N-terminal extension clearly differs among convicilins. Sequence comparison and phylogenetic analyses including convicilin and vicilin of Vicieae species indicated that the differentiation between vicilins and convicilins predated the differentiation of the two vicilin gene families (47- and 50-kDa vicilins), and that the N-terminal extension evolved mainly by a series of duplications of short internal sequences and triplet expansions, the predominant one being GAA.Key words: convicilin, evolution by duplications, Lens culinaris Medik., lentil, legumes, trinucleotide expansion.

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