The use of retrograde transport of horseradish peroxidase for studying the dendritic trees and axonal courses of particular groups of tract cells in the spinal cord

1984 ◽  
Vol 54 (3) ◽  
Author(s):  
T.P. Enevoldson ◽  
G. Gordon ◽  
D.J. Sanders
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Retrograde Transport ◽  
Dendritic Trees

The pattern of innervation in serially duplicated axolotl limbs: further evidence for the existence of local pathway cues?

Development ◽  
1987 ◽  
Vol 100 (3) ◽  
pp. 479-487
Author(s):  
N. Stephens ◽  
N. Holder
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Local Environment ◽  
Retrograde Transport ◽  
Limb Muscle ◽  
Biceps Muscle ◽  
Motor Innervation ◽  
Nerve Sprouting ◽  
Correct Pattern ◽  
Motor Neurones

The innervation of the biceps muscle was examined in regenerated and vitamin A-induced serially duplicated axolotl forelimbs using retrograde transport of horseradish peroxidase. The regenerated biceps muscle becomes innervated by motor neurones in the same position in the spinal cord as the normal biceps motor pool. In previous experiments in which the innervation of a second copy of a proximal limb muscle was examined in serially duplicated limbs (Stephens, Holder & Maden, 1985), the duplicate muscle was found to become innervated by motor neurones that would normally have innervated distal muscles. In the present study, the innervation of the second copy of biceps was examined under conditions designed to encourage nerve sprouting from ‘correct’ biceps axons. Following either partial limb denervation or denervation coupled with removal of the proximal biceps, the second copy of the muscle was still innervated by inappropriate motor neurones, which again would normally innervate distal limb muscles. These results are interpreted as evidence for the necessity for an appropriate local environment for axonal growth to allow reformation of a correct pattern of motor innervation in the regenerated limb.

Horseradish Peroxidase Studies in Animals with Neuromuscular Transpositions

1981 ◽  
Vol 90 (4) ◽  
pp. 396-397 ◽  
Author(s):  
G. David Neal ◽  
Dwight Sutton ◽  
Gregory Duncan ◽  
Charles W. Cummings
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Motor Neurons ◽  
Cervical Spinal Cord ◽  
Retrograde Transport ◽  
Laryngeal Nerve ◽  
Nucleus Ambiguus ◽  
Axonal Connections ◽  
Motor End Plate ◽  
Posterior Cricoarytenoid

Horseradish peroxidase (hrp) is used to trace axonal connections from the motor end-plate to the driving neuron. This technique has confirmed that the neurons activating the sternothyroid muscle are located in the cervical spinal cord, while those controlling the posterior cricoarytenoid (pca) are found in the nucleus ambiguus ipsilaterally. Eight rabbits underwent a sternothyroid ansa pedicle implantation to the pca at the time of sectioning the recurrent laryngeal nerve ipsilaterally. After two months, four of these animals received hrp injections into the previously implanted pca. Brainstem staining hrp did not reveal any retrograde transport to the motor neurons that were known to control the sternothyroid. Possible reasons for the failure of retrograde transport are discussed.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Axonal Transport of Horseradish Peroxidase in the Experimental Tethered Spinal Cord

1989 ◽  
Vol 15 (6) ◽  
pp. 296-301 ◽  
Author(s):  
Takaharu Fuse ◽  
John W. Patrickson ◽  
Shokei Yamada
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Axonal Transport ◽  
Tethered Spinal Cord
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