Traits of Panax ginseng hairy roots after cold storage and cryopreservation

1996 ◽  
Vol 15 (8) ◽  
pp. 555-560 ◽  
Author(s):  
Kayo Yoshimatsu ◽  
Hiroko Yamaguchi ◽  
Koichiro Shimomura
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Cold Storage

Traits of Panax ginseng hairy roots after cold storage and cryopreservation

1996 ◽  
Vol 15 (8) ◽  
pp. 555-560 ◽  
Author(s):  
Kayo Yoshimatsu ◽  
Hiroko Yamaguchi ◽  
Koichiro Shimomura
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Cold Storage

scholarly journals A stable beneficial symbiotic relationship between endophytic fungus Schizophyllum commune and host plant Panax ginseng

2017 ◽  
Author(s):  
Xin Zhai ◽  
Ling Chen ◽  
Min Jia ◽  
Changhui Li ◽  
Hui Shen ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Transcriptional Activity ◽  
Schizophyllum Commune ◽  
Chemical Substance ◽  
Stable Relationship ◽  
Expression Of Genes ◽  
Ginsenoside Rc

AbstractEndophytes and plants can establish specific long-term symbiosis through the accumulation of secondary metabolites. Interactions between microbial inhabitants represent a novel area of study for natural products research. In this study, a strain of endophyte 3R-2 that can enhance the biomass and contents of ginsenoside Rc, ginsenoside Rg2 and ginsenoside Rg3 of Panax ginseng hairy roots was screened out via HPLC, which was identified as Schizophyllum commune through the morphological and molecular identification. On the base, we found the infection of the endophyte were obviously observed widely in the P. ginseng and the strain formed a stable relationship with P. ginseng hairy roots in parenchyma cells around through tissues embedding slicing, HE ammonium silver staining and immunofluorescence staining. On the other hand, elicitors of fungus 3R-2 can also significantly promote hairy root growth and contents of several ginsenosides, even several times higher than 3R-2 mycelium did. Moreover, S. commune 3R-2 mycelium and its elicitor could enhance the transcriptional activity of key genes during the ginsenosides biosynthetic pathway dramatically. Thus, endophyte S. commune 3R-2 and its elicitor change the chemical substance content by regulating the expression of genes involved in the secondary metabolite biosynthetic pathway.

scholarly journals Ectopic Expression of Production of Anthocyanin Pigment 1 (PAP1) Improves the Antioxidant and Anti-Melanogenic Properties of Ginseng (Panax ginseng C.A. Meyer) Hairy Roots

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 922
Author(s):  
Sora Jin ◽  
Tae Kyung Hyun
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Differentially Expressed Gene ◽  
Ectopic Expression ◽  
Radical Scavenging ◽  
Ginseng Root ◽  
Anthocyanin Pigment ◽  
Anthocyanin Production

The development of genetically engineered cell cultures has been suggested as a potential approach for the production of target compounds from medicinal plants. In this study, we generated PAP1 (production of anthocyanin pigment 1)-overexpressing ginseng (Panax ginseng C.A. Meyer) hairy roots to improve the production of anthocyanins, as well as the bioactivity (e.g., antioxidant and whitening activities) of ginseng. Based on differentially expressed gene analysis, we found that ectopic expression of PAP1 induced the expression of genes involved in the ‘phenylpropanoid biosynthesis’ (24 genes), and ‘flavonoid biosynthesis’ (17 genes) pathways, resulting in 191- to 341-fold increases in anthocyanin production compared to transgenic control (TC) hairy roots. Additionally, PAP1-overexpressing ginseng hairy roots exhibited an approximately seven-fold higher DPPH-free radical scavenging activity and 10-fold higher ORAC value compared to the TC. In α-melanocyte-stimulating hormone-stimulated B16F10 cells, PAP1-overexpressing ginseng hairy roots strongly inhibited the accumulation of melanin by 50 to 59% compared to mock-control. Furthermore, results obtained by quantitative real-time PCR, western blot, and tyrosinase inhibition assay suggested that the anti-melanogenic activity of PAP1-overexpressing ginseng hairy roots is mediated by tyrosinase activity inhibition. Taken together, our results suggested that the ectopic expression of PAP1 is an effective strategy for the enhancement of anthocyanin production, which improves the biological activities of ginseng root cultures.

Comparison of Growth Characteristics of Panax ginseng Hairy Roots in Various Bioreactors

2003 ◽  
Vol 107 (1-3) ◽  
pp. 493-504 ◽  
Author(s):  
Gwi-Taek Jeong ◽  
Don-Hee Park ◽  
Baik Hwang ◽  
Je-Chang Woo
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Growth Characteristics

Optimum Conditions for Transformed Panax ginseng Hairy Roots in Flask Culture

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 1129-1140 ◽  
Author(s):  
Gwi-Taek Jeong ◽  
Don-Hee Park ◽  
Hwa-Won Ryu ◽  
Woo-Tai Lee ◽  
Kyungmoon Park ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Flask Culture ◽  
Optimum Conditions
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