scholarly journals Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

2017 ◽  
Vol 8 ◽  
Author(s):  
Jing An ◽  
Zhenmin Hu ◽  
Benning Che ◽  
Haiying Chen ◽  
Bingjun Yu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Heterologous Expression ◽  
Panax Ginseng ◽  
Hairy Roots ◽  
Soybean Cotyledon ◽  
Whole Plants

scholarly journals Salt-tolerance screening in Limonium sinuatum varieties with different flower colors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaojing Xu ◽  
Yingli Zhou ◽  
Ping Mi ◽  
Baoshan Wang ◽  
Fang Yuan
Keyword(s):  
Salt Tolerance ◽  
Photosynthetic Rate ◽  
Large Scale ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Salt Gland ◽  
Salt Glands ◽  
Chlorophyll Contents ◽  
Relative Water ◽  
Whole Plants

AbstractLimonium sinuatum, a member of Plumbaginaceae commonly known as sea lavender, is widely used as dried flower. Five L. sinuatum varieties with different flower colors (White, Blue, Pink, Yellow, and Purple) are found in saline regions and are widely cultivated in gardens. In the current study, we evaluated the salt tolerance of these varieties under 250 mmol/L NaCl (salt-tolerance threshold) treatment to identify the optimal variety suitable for planting in saline lands. After the measurement of the fresh weight (FW), dry weight (DW), contents of Na+, K+, Ca2+, Cl−, malondialdehyde (MDA), proline, soluble sugars, hydrogen peroxide (H2O2), relative water content, chlorophyll contents, net photosynthetic rate, and osmotic potential of whole plants, the salt-tolerance ability from strongest to weakest is identified as Pink, Yellow, Purple, White, and Blue. Photosynthetic rate was the most reliable and positive indicator of salt tolerance. The density of salt glands showed the greatest increase in Pink under NaCl treatment, indicating that Pink adapts to high-salt levels by enhancing salt gland formation. These results provide a theoretical basis for the large-scale planting of L. sinuatum in saline soils in the future.

scholarly journals GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean

2018 ◽  
Vol 17 (3) ◽  
pp. 530-538 ◽  
Author(s):  
Ming LI ◽  
Zheng HU ◽  
Qi-yan JIANG ◽  
Xian-jun SUN ◽  
Yuan GUO ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Hairy Roots

Traits of Panax ginseng hairy roots after cold storage and cryopreservation

1996 ◽  
Vol 15 (8) ◽  
pp. 555-560 ◽  
Author(s):  
Kayo Yoshimatsu ◽  
Hiroko Yamaguchi ◽  
Koichiro Shimomura
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Cold Storage

Effect of NaCl on the Growth of Whole Plants and Their Corresponding Callus Cultures

1981 ◽  
Vol 8 (3) ◽  
pp. 267 ◽  
Author(s):  
MK Smith ◽  
JA Mccomb
Keyword(s):  
Salt Tolerance ◽  
Callus Cultures ◽  
Solution Culture ◽  
Cellular Level ◽  
Plant Responses ◽  
Salt Glands ◽  
Salt Level ◽  
Whole Plant ◽  
Nutrient Solution Culture ◽  
Whole Plants

The effect of NaCl on growth was examined for whole plants and callus cultures of a salt-sensitive glycophyte (Phaseolus vulgaris L.), a salt-tolerant glycophyte (Beta vulgaris L.) and two halophytes (Atriplex undulata D. Dietr., which has salt glands, and Suaeda australis (R. Br.) Moq., a succulent). Whole plants were grown in nutrient solution culture at NaCl concentrations of 0.1-250 mM. Callus cultures were initiated from the same seed stock, and similar saline regimes were imposed. Whole plant responses were characteristic for the various types of plants: P. vulgaris showed a decrease in growth with increasing salinity; B. vulgaris showed a slight increase in growth at the intermediate salt level and a decrease at higher levels; A. undulata and S. australis showed well defined growth optima at 62.5 mM and 125 mM NaCl, respectively. Callus cultures of P. vulgaris and the two halophytes grew very poorly when salinity was increased. Callus of B. vulgaris showed the same tolerance to salt as did the whole plants. Thus salt tolerance of the halophytes depends on the anatomical and physiological complexity of the intact plant while callus from B. vulgaris appears to have a mechanism(s) of salt tolerance which operates at the cellular level.

scholarly journals A stable beneficial symbiotic relationship between endophytic fungus Schizophyllum commune and host plant Panax ginseng

2017 ◽  
Author(s):  
Xin Zhai ◽  
Ling Chen ◽  
Min Jia ◽  
Changhui Li ◽  
Hui Shen ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Transcriptional Activity ◽  
Schizophyllum Commune ◽  
Chemical Substance ◽  
Stable Relationship ◽  
Expression Of Genes ◽  
Ginsenoside Rc

AbstractEndophytes and plants can establish specific long-term symbiosis through the accumulation of secondary metabolites. Interactions between microbial inhabitants represent a novel area of study for natural products research. In this study, a strain of endophyte 3R-2 that can enhance the biomass and contents of ginsenoside Rc, ginsenoside Rg2 and ginsenoside Rg3 of Panax ginseng hairy roots was screened out via HPLC, which was identified as Schizophyllum commune through the morphological and molecular identification. On the base, we found the infection of the endophyte were obviously observed widely in the P. ginseng and the strain formed a stable relationship with P. ginseng hairy roots in parenchyma cells around through tissues embedding slicing, HE ammonium silver staining and immunofluorescence staining. On the other hand, elicitors of fungus 3R-2 can also significantly promote hairy root growth and contents of several ginsenosides, even several times higher than 3R-2 mycelium did. Moreover, S. commune 3R-2 mycelium and its elicitor could enhance the transcriptional activity of key genes during the ginsenosides biosynthetic pathway dramatically. Thus, endophyte S. commune 3R-2 and its elicitor change the chemical substance content by regulating the expression of genes involved in the secondary metabolite biosynthetic pathway.

scholarly journals Ectopic Expression of Production of Anthocyanin Pigment 1 (PAP1) Improves the Antioxidant and Anti-Melanogenic Properties of Ginseng (Panax ginseng C.A. Meyer) Hairy Roots

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 922
Author(s):  
Sora Jin ◽  
Tae Kyung Hyun
Keyword(s):  
Panax Ginseng ◽  
Hairy Roots ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Differentially Expressed Gene ◽  
Ectopic Expression ◽  
Radical Scavenging ◽  
Ginseng Root ◽  
Anthocyanin Pigment ◽  
Anthocyanin Production

The development of genetically engineered cell cultures has been suggested as a potential approach for the production of target compounds from medicinal plants. In this study, we generated PAP1 (production of anthocyanin pigment 1)-overexpressing ginseng (Panax ginseng C.A. Meyer) hairy roots to improve the production of anthocyanins, as well as the bioactivity (e.g., antioxidant and whitening activities) of ginseng. Based on differentially expressed gene analysis, we found that ectopic expression of PAP1 induced the expression of genes involved in the ‘phenylpropanoid biosynthesis’ (24 genes), and ‘flavonoid biosynthesis’ (17 genes) pathways, resulting in 191- to 341-fold increases in anthocyanin production compared to transgenic control (TC) hairy roots. Additionally, PAP1-overexpressing ginseng hairy roots exhibited an approximately seven-fold higher DPPH-free radical scavenging activity and 10-fold higher ORAC value compared to the TC. In α-melanocyte-stimulating hormone-stimulated B16F10 cells, PAP1-overexpressing ginseng hairy roots strongly inhibited the accumulation of melanin by 50 to 59% compared to mock-control. Furthermore, results obtained by quantitative real-time PCR, western blot, and tyrosinase inhibition assay suggested that the anti-melanogenic activity of PAP1-overexpressing ginseng hairy roots is mediated by tyrosinase activity inhibition. Taken together, our results suggested that the ectopic expression of PAP1 is an effective strategy for the enhancement of anthocyanin production, which improves the biological activities of ginseng root cultures.

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