Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.)

1991 ◽  
Vol 9 (11) ◽  
Author(s):  
Yin-Fu Chang ◽  
WenChung Wang ◽  
ColleenY. Warfield ◽  
HenryT. Nguyen ◽  
JimR. Wong
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Cell Cultures ◽  
Triticum Aestivum L

The effects of level of 2,4-D and time in culture on regeneration rate and chromosome numbers of regenerants from calli of the hybrid Triticum aestivum cv. Chinese Spring ph1b × Thinopyrum ponticum (2n = 10x = 70)

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 166-172 ◽  
Author(s):  
D. Bai ◽  
D. R. Knott
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Culture ◽  
Chromosome Numbers ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Root Tip ◽  
Regeneration Rate ◽  
Thinopyrum Ponticum

Thinopyrum ponticum (Podp.) Barkworth &D. R. Dewey (2n = 10x = 70) has excellent resistance for both leaf and stem rusts. Long-term callus cultures were established from the immature embryos of a hybrid between Triticum aestivum L. (2n = 6x = 42) × Th. ponticum. They were maintained in culture for over 2 years and continued to grow and have organogenetic capacity. With increasing time on a maintenance medium, the plant regeneration rates of the hybrid calli decreased when transferred to regeneration media containing 0.1, 0.2, or 0.5 mg/L 2,4-D, but the rate of decrease was much higher at 0.5 mg/L than at either 0.1 or 0.2 mg/L 2,4-D. After 3 months of subculture, the highest plant regeneration rate was obtained on the medium containing 0.5 mg/L 2,4-D (1.11 plantlets/callus), while on the 24th month of subculture the highest plant regeneration rate was obtained on the medium containing 0.1 mg/L 2,4-D (0.20 plantlets/callus). Thus, it was shown that as the calli aged it was important to reduce the level of 2,4-D in the regeneration medium. Over 2 years, a total of 667 regenerants were successfully transferred and grown to maturity. Chromosome numbers in root-tip cells were determined for 539 regenerants and ranged from 36 to 70. Telocentric chromosomes were frequent. A fertile plant was found among the regenerants after 15 months of subculture. It had 56 chromosomes with 2.15 (1–6) univalents, 22.76 (17–26) closed bivalents, 3.55 (1–9) open bivalents, and 0.41 (0–3) trivalents and was highly resistant to stem rust race 15B-1. Callus culture of wide hybrids can be used to introgress characters from alien species into wheat.Key words: wheat, callus culture, long-term regeneration, wide hybridization.

scholarly journals Tracking Changes on Soil Structure and Organic Carbon Sequestration after 30 Years of Different Tillage and Management Practices

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 291
Author(s):  
Ramón Bienes ◽  
Maria Jose Marques ◽  
Blanca Sastre ◽  
Andrés García-Díaz ◽  
Iris Esparza ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Organic Carbon ◽  
Management Practices ◽  
Management Strategies ◽  
Triticum Aestivum L ◽  
Field Trials ◽  
Conventional Tillage ◽  
Soil Parameters ◽  
Wilting Point

Long-term field trials are essential for monitoring the effects of sustainable land management strategies for adaptation and mitigation to climate change. The influence of more than thirty years of different management is analyzed on extensive crops under three tillage systems, conventional tillage (CT), minimum tillage (MT), and no-tillage (NT), and with two crop rotations, monoculture winter-wheat (Triticum aestivum L.) and wheat-vetch (Triticum aestivum L.-Vicia sativa L.), widely present in the center of Spain. The soil under NT experienced the largest change in organic carbon (SOC) sequestration, macroaggregate stability, and bulk density. In the MT and NT treatments, SOC content was still increasing after 32 years, being 26.5 and 32.2 Mg ha−1, respectively, compared to 20.8 Mg ha−1 in CT. The SOC stratification (ratio of SOC at the topsoil/SOC at the layer underneath), an indicator of soil conservation, increased with decreasing tillage intensity (2.32, 1.36, and 1.01 for NT, MT, and CT respectively). Tillage intensity affected the majority of soil parameters, except the water stable aggregates, infiltration, and porosity. The NT treatment increased available water, but only in monocropping. More water was retained at the permanent wilting point in NT treatments, which can be a disadvantage in dry periods of these edaphoclimatic conditions.

scholarly journals Long-Term Winter Wheat (Triticum aestivum L.) Seasonal Irrigation Amount, Evapotranspiration, Yield, and Water Productivity under Semiarid Climate

Agronomy ◽  
2018 ◽  
Vol 8 (6) ◽  
pp. 96 ◽  
Author(s):  
Koffi Djaman ◽  
Michael O’Neill ◽  
Curtis Owen ◽  
Daniel Smeal ◽  
Margaret West ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Water Productivity ◽  
Triticum Aestivum L ◽  
Irrigation Amount ◽  
Semiarid Climate ◽  
Seasonal Irrigation
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