Ultrastructural enzyme-cytochemical study of the intrinsic glandular cells in the corpus cardiacum of Locusta migratoria: relation to the secretory and endocytotic pathways, and to the lysosomal system

1989 ◽  
Vol 255 (1) ◽  
Author(s):  
W.F. Jansen ◽  
J.H.B. Diederen ◽  
M. Dorland ◽  
J. Langermans ◽  
B.P.M. Meesen ◽  
...  
Keyword(s):  
Locusta Migratoria ◽  
Corpus Cardiacum ◽  
Cytochemical Study ◽  
Glandular Cells ◽  
Lysosomal System

ULTRASTRUCTURAL STUDIES ON FORMAMIDINE-INDUCED RELEASE OF NEUROSECRETION IN LOCUSTA MIGRATORIA (ORTHOPTERA: LOCUSTIDAE)

1983 ◽  
Vol 115 (9) ◽  
pp. 1147-1153 ◽  
Author(s):  
Gur Jai Pal Singh ◽  
John F. Barker
Keyword(s):  
Plasma Membrane ◽  
Locusta Migratoria ◽  
Glandular Cell ◽  
Neurosecretory Material ◽  
Hormone Release ◽  
Corpus Cardiacum ◽  
Ultrastructural Studies ◽  
Glandular Cells

AbstractThe action of a formamidine pesticide on the ultrastructure of hormone release from the glandular lobe of the corpus cardiacum (CC) of the locust, Locusta migratoria (L.), has been studied. Treatment of the isolated CC with 5 μM desmethylchlordimeform (DCDM) caused depletion of neurosecretory material from the intrinsic cells of the glandular lobe without affecting the nervus corpus cardiacum II axons. Pretreatment of isolated CC with the α-aminergic antagonist (5 μM) phentolamine blocked DCDM-induced release of neurosecretory material from the glandular cells. DCDM-induced release of neurosecretion from the glandular cell axons occurred via exocytosis. DCDM treatment also affected the distribution of mitochondria. Mitochondria from adjacent cells lined up along the plasma membrane, forming pairs in juxtaposition.

The Control of Changes in Peripheral Sensilla Associated with Feeding in Locusta Migratoria (L.)

1972 ◽  
Vol 57 (3) ◽  
pp. 755-763
Author(s):  
E. A. BERNAYS ◽  
R. F. CHAPMAN
Keyword(s):  
Normal Level ◽  
Electrical Resistance ◽  
Locusta Migratoria ◽  
Corpus Cardiacum ◽  
Corpora Cardiaca ◽  
Diuretic Hormone ◽  
Stretch Receptors ◽  
The Brain ◽  
Maxillary Palps ◽  
Stimulation Of

1. The electrical resistance across the tips of the maxillary palps is not affected by stimulation of the palps, but increases to the normal level found after feeding as a result of distension of the foregut with agar or injection of corpus cardiacum homogenates into the haemolymph. 2. No increase in resistance occurs if the posterior pharyngeal nerves or the frontal connectives are cut. 3. It is inferred that distension of the foregut stimulates stretch receptors which, acting via the posterior pharyngeal nerves, the frontal connectives and the brain, cause the release of hormone from the storage lobes of the corpora cardiaca. This hormone acts on the terminal sensilla of the palps, causing them to close and so increasing the resistance across the palps. 4. Release of the diuretic hormone is controlled via the same pathway.

scholarly journals Pathways followed by membrane recovered from the surface of plasma cells and myeloma cells

1980 ◽  
Vol 152 (1) ◽  
pp. 1-19 ◽  
Author(s):  
PD Ottosen ◽  
PJ Courtoy ◽  
MG Farquhar
Keyword(s):  
Secretory Pathway ◽  
Plasma Cells ◽  
Surface Membrane ◽  
Secretory Cells ◽  
Secretory Product ◽  
Vesicle Membrane ◽  
Myeloma Cells ◽  
Glandular Cells ◽  
Lysosomal System ◽  
Endocytic Vesicles

Evidence for recovery of surface membrane and its fusion with Golgi cisternae has been obtained previously in several glandular cells. This study was conducted to determine whether or not membrane is similarly retrieved from the surfaces of plasma cells from lymph nodes (of rats immunized with horseradish peroxidase [HRP]) and mouse myeloma cells (RPC 5.4 and X63 Ag 8 cell lines). Electron-dense tracers (cationic and anionic ferritin, HRP) were used to trace the pathways followed by surface membrane recovered by endocytosis, and immunocytochemistry was used to identify the secretory compartments. When plasma cells or myeloma cells were incubated with cationized ferritin (CF), it bound to the cell surfaces and was taken up in endocytic vesicles, for the most part bound to the vesicle membrane. After 30-60 min, it was found increasingly within lysosomes and in several secretory compartments- notably in multiple stacked Golgi cisternae and secretory vacuoles. By immunocytochemistry the secretory product (immunoglobulins) and CF could be demonstrated in the same Golgi components. When myeloma cells were incubated with native (anionic) ferritin or in HRP, these tracers were taken up in much smaller amounts, primarily within the contents of endocytic vesicles. With continued incubation, they appeared only in lysosomes. When cells were doubly incubated, first in CF and then in HRP, both tracers were taken up (often within the same endocytic vesicle), but they maintained their same destinations as when incubated in a single tracer alone: the content marker, HRP, was localized exclusively within the lysosomal system, whereas the membrane marker, CF, was found within elements along the secretory pathway as well as within lysosomes. The findings demonstrate the existence of considerable membrane traffic between the cell membrane and the Golgi cisternae and lysosomes in both normal plasma cells and myeloma cells. Because myeloma cells behave like the glandular cells studied previously with regard to pathways of retrieved surface membrane, they represent a suitable and promising system for further studies of mechanisms and pathways of membrane retrieval and recycling in secretory cells.

Peptide profiling of a single Locusta migratoria corpus cardiacum by nano-LC tandem mass spectrometry

Peptides ◽  
2003 ◽  
Vol 24 (10) ◽  
pp. 1475-1485 ◽  
Author(s):  
G. Baggerman ◽  
E. Clynen ◽  
J. Huybrechts ◽  
P. Verleyen ◽  
S. Clerens ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Locusta Migratoria ◽  
Tandem Mass ◽  
Corpus Cardiacum
Sign in / Sign up

Export Citation Format

Share Document