Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH

1986 ◽  
Vol 243 (1) ◽  
Author(s):  
H. Schooneveld ◽  
H.M. Romberg-Privee ◽  
J.A. Veenstra
Keyword(s):  
Periplaneta Americana ◽  
Locusta Migratoria ◽  
Corpus Cardiacum ◽  
Adipokinetic Hormone ◽  
Specific Antisera ◽  
Glandular Cells

scholarly journals Isolation and structure of a novel charged member of the red–pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera)

1990 ◽  
Vol 269 (2) ◽  
pp. 309-313 ◽  
Author(s):  
G Gäde ◽  
H Wilps ◽  
R Kellner
Keyword(s):  
Periplaneta Americana ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Reversed Phase ◽  
American Cockroach ◽  
Red Pigment ◽  
Adipokinetic Hormone ◽  
Migratory Locust ◽  
Corpora Cardiaca ◽  
Adipokinetic Hormone Family

A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named ‘Phormia terraenovae hypertrehalosaemic hormone’ (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.

scholarly journals A unique charged tyrosine-containing member of the adipokinetic hormone/red-pigment-concentrating hormone peptide family isolated and sequenced from two beetle species

1991 ◽  
Vol 275 (3) ◽  
pp. 671-677 ◽  
Author(s):  
G Gäde
Keyword(s):  
Periplaneta Americana ◽  
Locusta Migratoria ◽  
Structural Features ◽  
American Cockroach ◽  
Beetle Species ◽  
Red Pigment ◽  
Adipokinetic Hormone ◽  
Migratory Locust ◽  
Melolontha Melolontha ◽  
C Terminus

An identical neuropeptide was isolated from the corpora cardiaca of two beetle species, Melolontha melolontha and Geotrupes stercorosus. Its primary structure was determined by pulsed-liquid-phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The sequence of this peptide, which is designated Mem-CC, is pGlu-Leu-Asn-Tyr-Ser-Pro-Asp-Trp-NH2. It is a new member of the adipokinetic hormone/red-pigment-concentrating hormone (AKH/RPCH) family of peptides with two unusual structural features: it is charged and contains a tyrosine residue at position 4, where all other family members have a phenylalanine residue. Structure-activity studies in the migratory locust (Locusta migratoria) and the American cockroach (Periplaneta americana) revealed that the peptide was poorly active, owing to its structural uniqueness.

ULTRASTRUCTURAL STUDIES ON FORMAMIDINE-INDUCED RELEASE OF NEUROSECRETION IN LOCUSTA MIGRATORIA (ORTHOPTERA: LOCUSTIDAE)

1983 ◽  
Vol 115 (9) ◽  
pp. 1147-1153 ◽  
Author(s):  
Gur Jai Pal Singh ◽  
John F. Barker
Keyword(s):  
Plasma Membrane ◽  
Locusta Migratoria ◽  
Glandular Cell ◽  
Neurosecretory Material ◽  
Hormone Release ◽  
Corpus Cardiacum ◽  
Ultrastructural Studies ◽  
Glandular Cells

AbstractThe action of a formamidine pesticide on the ultrastructure of hormone release from the glandular lobe of the corpus cardiacum (CC) of the locust, Locusta migratoria (L.), has been studied. Treatment of the isolated CC with 5 μM desmethylchlordimeform (DCDM) caused depletion of neurosecretory material from the intrinsic cells of the glandular lobe without affecting the nervus corpus cardiacum II axons. Pretreatment of isolated CC with the α-aminergic antagonist (5 μM) phentolamine blocked DCDM-induced release of neurosecretory material from the glandular cells. DCDM-induced release of neurosecretion from the glandular cell axons occurred via exocytosis. DCDM treatment also affected the distribution of mitochondria. Mitochondria from adjacent cells lined up along the plasma membrane, forming pairs in juxtaposition.

Interspecificity of lipid-regulating factors from insect corpus cardiacum

1972 ◽  
Vol 50 (1) ◽  
pp. 63-65 ◽  
Author(s):  
R. G. H. Downer
Keyword(s):  
Adult Male ◽  
Periplaneta Americana ◽  
Locusta Migratoria ◽  
Aqueous Extracts ◽  
Corpus Cardiacum ◽  
Site Of Action

Aqueous extracts of corpus cardiacum from Periplaneta americana L. elicit the characteristic locust response of hyperlipemia when injected into adult male Locusta migratoria migratoides R. and F. Conversely, gland extracts of L. m. migratoides cause the typical cockroach response of hypolipemia when injected into adult male P. americana.It is proposed that similar factors are present in both species with specificity being determined at the site of action.

Sign in / Sign up

Export Citation Format

Share Document