Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris

1995 ◽  
Vol 90 (6) ◽  
pp. 885-891 ◽  
Author(s):  
E. M. J. Salentijn ◽  
M. J. B. Arens-De Reuver ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
W. J. Stiekema ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Cyst Nematode ◽  
Chromosome 1 ◽  
Resistance Locus ◽  
Beet Cyst Nematode ◽  
Isolation And Characterization

Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens

1994 ◽  
Vol 89 (4) ◽  
pp. 459-466 ◽  
Author(s):  
E. M. J. Salentijn ◽  
N. N. Sandal ◽  
R. Klein-Lankhorst ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
...  
Keyword(s):  
Long Range ◽  
Satellite Dna ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Chromosome 1 ◽  
Resistance Locus ◽  
Beet Cyst Nematode

Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens

1992 ◽  
Vol 235 (2-3) ◽  
pp. 432-440 ◽  
Author(s):  
E. M. J. Salentijn ◽  
N. N. Sandal ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
F. A. Krens ◽  
...  
Keyword(s):  
Dna Markers ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Resistance Locus ◽  
Beta Procumbens ◽  
Beet Cyst Nematode ◽  
Beta Patellaris

Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Ik-Young Choi ◽  
Prakash Basnet ◽  
Hana Yoo ◽  
Neha Samir Roy ◽  
Rahul Vasudeo Ramekar ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Gene Families ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Soybean Breeding ◽  
Resistant Varieties ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Scn Resistance

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.

scholarly journals Isolation and Characterization Of Rice R Genes: Evidence for Distinct Evolutionary Paths in Rice and Maize

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 1021-1031 ◽  
Author(s):  
Jianping Hu ◽  
Beth Anderson ◽  
Susan R Wessler
Keyword(s):  
Gene Family ◽  
Gene Families ◽  
Chromosome 1 ◽  
R Gene ◽  
R Genes ◽  
Helix Loop Helix ◽  
Isolation And Characterization ◽  
Undetermined Function ◽  
Evolutionary Paths

Abstract R and B genes and their homologues encode basic helix-loop-helix (bHLH) transcriptional activators that regulate the anthocyanin biosynthetic pathway in flowering plants. In maize, R/B genes comprise a very small gene family whose organization reflects the unique evolutionary history and genome architecture of maize. To know whether the organization of the R gene family could provide information about the origins of the distantly related grass rice, we characterized members of the R gene family from rice Oryza sativa. Despite being a true diploid, O. sativa has at least two R genes. An active homologue (Ra) with extensive homology with other R genes is located at a position on chromosome 4 previously shown to be in synteny with regions of maize chromosomes 2 and 10 that contain the B and R loci, respectively. A second rice R gene (Rb) of undetermined function was identified on chromosome 1 and found to be present only in rice species with AA genomes. All non-AA species have but one R gene that is Ra-like. These data suggest that the common ancestor shared by maize and rice had a single R gene and that the small R gene families of grasses have arisen recently and independently.

scholarly journals Use of isozymes as chromosome markers in the isolation and characterization of wheat-barley chromosome addition lines

1980 ◽  
Vol 36 (3) ◽  
pp. 311-325 ◽  
Author(s):  
Gary E. Hart ◽  
A. K. M. R. Islam ◽  
K. W. Shepherd
Keyword(s):  
Chinese Spring ◽  
Chromosome 1 ◽  
Addition Lines ◽  
Chromosome 4 ◽  
Glutamic Oxaloacetic Transaminase ◽  
Chromosome Addition ◽  
Isolation And Characterization ◽  
Chinese Spring Wheat ◽  
Chromosome Addition Lines

SUMMARYThe alcohol dehydrogenase (ADH), glutamic oxaloacetic transaminase (GOT), aminopeptidase (AMP), endopeptidase (EP), and esterase (EST) zymogram phenotypes of Chinese Spring wheat, Betzes barley, Chinese Spring-Betzes heptaploids, and a number of presumptive Betzes chromosome additions to Chinese Spring were determined. It was found that four disomic chromosome addition lines could be distinguished from one another and from the other three possible lines on the basis of the zymogram phenotypes of these isozymes.The structural gene Adh-H1 was located in Betzes chromosome 4, the genes Got-H2 and Amp-H1 in chromosome 6, and the gene Ep-H1 in chromosome 1. These gene locations provide evidence of homoeology between Betzes chromosomes 4, 6, and 1 and the Chinese Spring chromosomes of homoeologous groups 4, 6, and 7, respectively.

Isolation and characterization of a putative collagen gene from the potato cyst nematode Globodera pallida

Parasitology ◽  
1996 ◽  
Vol 113 (6) ◽  
pp. 581-588 ◽  
Author(s):  
J. T. Jones ◽  
R. H. Curtis ◽  
P. J. Wightman ◽  
P. R. Burrows
Keyword(s):  
Cyst Nematode ◽  
Globodera Pallida ◽  
Collagen Gene ◽  
Expression Library ◽  
Northern Blots ◽  
Isolation And Characterization ◽  
Collagen Genes ◽  
Mixed Stage

SUMMARYA cDNA clone encoding a full length putative collagen has been isolated in a screen of a mixed stage Globodera pallida expression library. Comparison of the deduced amino acid sequence of this molecule with other collagens suggests it is a cuticular collagen and a member of the col-8 subfamily of collagen genes. Northern blots show the gene is expressed specifically in gravid, adult females of the parasite as compared to second (invasive) stage juveniles and virgin females. Preliminary immunocytochemical studies indicate this collagen is present in areas other than the cuticle; these findings and the potential functional role of this collagen are discussed.

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