Fine structural localization of nucleoside triphosphatase and acid phosphatase activity in Trichomonas vaginalis donn�

1974 ◽  
Vol 151 (3) ◽  
Author(s):  
M.H. Nielsen
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Trichomonas Vaginalis ◽  
Structural Localization ◽  
Nucleoside Triphosphatase

Trichomonas vaginalis: Determination of Acid Phosphatase Activity as a Pharmacological Screening Procedure

2003 ◽  
Vol 89 (5) ◽  
pp. 1076-1077 ◽  
Author(s):  
M. M. Martínez-Grueiro ◽  
D. Montero-Pereira ◽  
C. Giménez-Pardo ◽  
J. J. Nogal-Ruiz ◽  
J. A. Escario ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Trichomonas Vaginalis ◽  
Screening Procedure ◽  
Pharmacological Screening

scholarly journals Fine Structural Localization of Acid Phosphatase Activity in Myeloma Cells

1968 ◽  
Vol 96 (4) ◽  
pp. 399-403 ◽  
Author(s):  
Akira B. Miura ◽  
Atsuo Suzuki ◽  
Seiju Onodera ◽  
Shinobu Sakamoto ◽  
Chiyuki Suzuki ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Structural Localization ◽  
Myeloma Cells

scholarly journals FINE STRUCTURAL LOCALIZATION OF ACID AND ALKALINE PHOSPHATASES IN CELLS OF RABBIT BLOOD AND BONE MARROW

1967 ◽  
Vol 15 (6) ◽  
pp. 311-334 ◽  
Author(s):  
B. K. WETZEL ◽  
S. S. SPICER ◽  
R. G. HORN
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Mononuclear Cells ◽  
Nuclear Staining ◽  
Alkaline Phosphatases ◽  
Structural Localization ◽  
Dense Granules ◽  
Dense Bodies

In rabbit heterophils, acid phosphatase activity occurs in primary (azurophil) granules which predominate in early cells and persist in mature cells and in tertiary granules which are seen only in mature cells. Alkaline phosphatase activity occurs in secondary granules which appear in intermediate heterophils and later predominate in mature cells. Acid phosphatase activity in heterophil Golgi zones coincides developmentally with the genesis of primary and, later, tertiary granules, whereas alkaline phosphatase in the Golgi complex coincides with secondary granulogenesis. In developing eosinophils, acid phosphatase reaction product occurs in Golgi elements, rims the spherical precursors of angular, mature granules and appears inconsistently within mature granules. Basophil myelocytes show acid phosphatase in Golgi elements but not in specific granules. Additional acid phosphatase reactive structures include: granules of mononuclear cells; phagocytic vacuoles in macrophages; autophagic vacuoles in maturing erythroid cells; small dense granules of platelets; dense bodies in lipocytes; and Golgi elements of mononuclear cells, macrophages, nucleated red cells, megakaryocytes and lipocytes. Localized deposits were absent in control specimens except for enzyme-independent nuclear staining in alkaline phosphatase preparations.

scholarly journals THE FINE STRUCTURAL LOCALIZATION OF ALKALINE AND ACID PHOSPHATASE ACTIVITY IN THE RAT SUBMANDIBULAR GLAND

1968 ◽  
Vol 16 (9) ◽  
pp. 572-581 ◽  
Author(s):  
BRUCE I. BOGART
Keyword(s):  
Alkaline Phosphatase ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Myoepithelial Cell ◽  
Structural Localization ◽  
Pinocytotic Vesicles ◽  
Rat Submandibular Gland

The lead capture method was employed to study the fine structural localization of nonspecific phosphatase activity in the rat submandibular gland. Alkaline phosphatase activity was observed in association with the plasma membrane and pinocytotic vesicles of the myoepithelial cell. A polarity in the distribution of alkaline phosphatase activity was described along the myoepithelial cell process. More reaction product was observed in association with the plasma membrane on the parenchymal surface than on the plamsa membrane on the stromal surface, where reaction product was confined mostly to the pinocytotic vesicles. Activity was evenly distributed over both surfaces of the portion of the myoepithelial cell characterized by the nucleus. Activity was also observed to be associated with pinocytotic vesicles of the endothelial cells and the plasma membrane of erythrocytes. No activity was observed in association with the ductal elements. Acid phosphatase activity was associated with membrane-bound structures in the acini and ducts. These structures took the form of lipofuscin granules in the acini and either multivesicular or dense bodies in the ducts.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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