scholarly journals Tissue culture-induced DNA methylation polymorphisms in repetitive DNA of tomato calli and regenerated plants

1995 ◽  
Vol 91 (8) ◽  
pp. 1257-1264 ◽  
Author(s):  
M. J. M. Smulders ◽  
W. Rus-Kortekaas ◽  
B. Vosman
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Repetitive Dna ◽  
Regenerated Plants

DNA methylation in plants and its role in tissue culture

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 717-729 ◽  
Author(s):  
P. T. H. Brown
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Zea Mays ◽  
Somaclonal Variation ◽  
Inbred Line ◽  
Phenotypic Variation ◽  
Methylation Status ◽  
Structural Genes ◽  
Gene Methylation ◽  
Regenerated Plants

Regeneration of plants via tissue culture often results in a number of plants subsequently showing phenotypic or genotypic deviations from the parental type. This variation has been called somaclonal variation. In an analysis of regenerated Zea mays plants of the inbred line A188, high levels of phenotypic variation were observed. Subsequent analysis of these regenerated plants shows that a high proportion of the regenerants demonstrate significant alterations in the methylation status of both housekeeping and structural genes. These results are described and the theory of gene methylation is reviewed with regard to the differences that exist between plant and animal systems.Key words: 5-methylcytosine, 5-azacytidine, tissue culture, cereals, somaclonal variation.

DNA methylation in satellite repeats disorders

2019 ◽  
Vol 63 (6) ◽  
pp. 757-771 ◽  
Author(s):  
Claire Francastel ◽  
Frédérique Magdinier
Keyword(s):  
Dna Methylation ◽  
Human Genome ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Repeats ◽  
Tremendous Progress ◽  
Genes Encoding ◽  
Dna Elements ◽  
Near Future

Abstract Despite the tremendous progress made in recent years in assembling the human genome, tandemly repeated DNA elements remain poorly characterized. These sequences account for the vast majority of methylated sites in the human genome and their methylated state is necessary for this repetitive DNA to function properly and to maintain genome integrity. Furthermore, recent advances highlight the emerging role of these sequences in regulating the functions of the human genome and its variability during evolution, among individuals, or in disease susceptibility. In addition, a number of inherited rare diseases are directly linked to the alteration of some of these repetitive DNA sequences, either through changes in the organization or size of the tandem repeat arrays or through mutations in genes encoding chromatin modifiers involved in the epigenetic regulation of these elements. Although largely overlooked so far in the functional annotation of the human genome, satellite elements play key roles in its architectural and topological organization. This includes functions as boundary elements delimitating functional domains or assembly of repressive nuclear compartments, with local or distal impact on gene expression. Thus, the consideration of satellite repeats organization and their associated epigenetic landmarks, including DNA methylation (DNAme), will become unavoidable in the near future to fully decipher human phenotypes and associated diseases.

Comparative Tissue Culture Response of Wheat Cultivars and Evaluation of Regenerated Plants

2004 ◽  
Vol 7 (3) ◽  
pp. 406-408 ◽  
Author(s):  
Muhammad Farooq . ◽  
Hamid Rashid . ◽  
Ihsanullah . ◽  
Zubeda Chaudhry . ◽  
Khan Bahadar Marwat .
Keyword(s):  
Tissue Culture ◽  
Wheat Cultivars ◽  
Tissue Culture Response ◽  
Regenerated Plants

scholarly journals Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

2020 ◽  
Vol 21 (20) ◽  
pp. 7459
Author(s):  
María Elena González-Benito ◽  
Miguel Ángel Ibáñez ◽  
Michela Pirredda ◽  
Sara Mira ◽  
Carmen Martín
Keyword(s):  
Dna Methylation ◽  
In Situ Conservation ◽  
Plant Conservation ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Regenerated Plants ◽  
Before And After ◽  
Methylation Patterns

Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

Assessment of DNA methylation changes in tissue culture of Brassica napus

2014 ◽  
Vol 50 (11) ◽  
pp. 1186-1191 ◽  
Author(s):  
Y. Gao ◽  
L. Ran ◽  
Y. Kong ◽  
J. Jiang ◽  
V. Sokolov ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Brassica Napus

scholarly journals A repetitive DNA fragment carrying a hot spot for de novo DNA methylation enhances expression variegation in tobacco and petunia

1995 ◽  
Vol 8 (6) ◽  
pp. 919-932 ◽  
Author(s):  
Michael ten Lohuis ◽  
Andreas Müller ◽  
Iris Heidmann ◽  
Ingrid Niedenhof ◽  
Peter Meyer
Keyword(s):  
Dna Methylation ◽  
Repetitive Dna ◽  
De Novo ◽  
Hot Spot ◽  
Dna Fragment

Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L.

1995 ◽  
Vol 91 (3) ◽  
pp. 395-400 ◽  
Author(s):  
L. Natali ◽  
T. Giordani ◽  
G. Cionini ◽  
C. Pugliesi ◽  
M. Fambrini ◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Repetitive Dna ◽  
Frequency Variation ◽  
Helianthus Annuus L ◽  
Regenerated Plants

DNA methylation in lowbush blueberry (Vaccinium angustifolium Ait.) propagated by softwood cutting and tissue culture

2018 ◽  
Vol 98 (5) ◽  
pp. 1035-1044 ◽  
Author(s):  
Juran C. Goyali ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Growth Conditions ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Restriction Sites ◽  
Methylation Sensitive Amplification Polymorphism ◽  
Softwood Cutting

Plant DNA methylation is one of the frequent epigenetic variations induced by tissue culture. Global DNA methylation was evaluated in lowbush blueberry (Vaccinium angustifolium Ait.) wild clone QB9C and cultivar Fundy propagated by conventional softwood cutting (SC) and tissue culture (TC) using the methylation-sensitive amplification polymorphism (MSAP) technique. In all, 106 and 107 DNA fragments were amplified using 16 selective primer combinations in SC plants of QB9C and Fundy, respectively. In micropropagated QB9C and Fundy plants, there were 105 and 109 amplified fragments, respectively. Overall, 25% of restriction sites were methylated at the cytosine nucleotide in QB9C plants propagated by SC compared with 19% in Fundy. In contrast, a total of 29% and 20% of restriction sites were methylated at cytosine in micropropagated QB9C and Fundy plants, respectively. Tissue culture plants demonstrated higher methylation events than SC plants in both genotypes. Previously, methylation polymorphism has been detected in TC plants but not in SC counterparts. Different patterns of DNA methylation and polymorphism in the plants propagated in in vitro and in vivo conditions suggest the possibility of involvement of these fragments in the processes of regulating plant growth and development under prevailing growth conditions.

Sign in / Sign up

Export Citation Format

Share Document