Identification of two RAPD markers tightly linked with the Nicotiana debneyi gene for resistance to black root rot of tobacco

1995 ◽  
Vol 91 (8) ◽  
pp. 1184-1189 ◽  
Author(s):  
D. Bai ◽  
R. Reeleder ◽  
J. E. Brandie
Keyword(s):  
Root Rot ◽  
Rapd Markers ◽  
Black Root Rot ◽  
Nicotiana Debneyi

Effect of the Nicotiana debneyi black root rot resistance gene on the yield and quality characteristics of flue-cured tobacco in Ontario

2003 ◽  
Vol 83 (4) ◽  
pp. 939-942 ◽  
Author(s):  
H. M. Haji ◽  
R. A. Brammall ◽  
D. L. VanHooren
Keyword(s):  
Root Rot ◽  
Quality Characteristics ◽  
Economic Returns ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Yield And Quality ◽  
Chalara Elegans ◽  
Yield Quality ◽  
Nicotiana Debneyi ◽  
Rot Disease

The effects of Nicotiana debneyi-derived resistance to black root rot disease were evaluated for yield, agronomic and quality traits by comparing the near isogenic cultivars AC Gayed (resistant) and Delgold (susceptible). Over 7 yr of trials the possession of resistance led to yields and economic returns that averaged 6 and 7% lower, respectively, than for the susceptible line. Key words: Flue-cured tobacco, Nicotiana tabaccum, Black Root Rot, Chalara elegans, Nicotiana debneyi, yield, quality

AC Gayed flue-cured tobacco

1997 ◽  
Vol 77 (1) ◽  
pp. 157-158 ◽  
Author(s):  
J. E. Brandle ◽  
W. D. Rogers ◽  
J. C. D. Ankersmit
Keyword(s):  
Nicotiana Tabacum ◽  
Root Rot ◽  
Research Centre ◽  
Black Root Rot ◽  
Chalara Elegans ◽  
Tobacco Production ◽  
Nicotiana Debneyi ◽  
Production Areas ◽  
Grade Index ◽  
Disease Free

AC Gayed is a flue-cured tobacco (Nicotiana tabacum) cultivar bred at the Pest Management Research Centre of Agriculture and Agri-Food Canada. It is a a black root rot (Chalara elegans) resistant, backcross derivative of Delgold (ST205/PB19//*5 Delgold) (Pandeya and White 1984). The resistance gene originated from Nicotiana debneyi. AC Gayed is adapted to the tobacco production areas of Ontario. It has similar grade index, but slightly lower yield than Delgold when grown in disease-free conditions. Key words: Nicotiana tabacum, black root rot resistance, backcross

Three novel Nicotiana debneyi specific repetitive DNA elements derived from a RAPD marker

Genome ◽  
1999 ◽  
Vol 42 (1) ◽  
pp. 104-109 ◽  
Author(s):  
D Bai
Keyword(s):  
Repetitive Dna ◽  
Root Rot ◽  
Somatic Hybrids ◽  
Rapd Marker ◽  
Translocation Line ◽  
Black Root Rot ◽  
Dna Elements ◽  
Nicotiana Debneyi ◽  
Species Specific ◽  
Repetitive Dna Elements

The RAPD marker UBC4181050, tightly linked in coupling with the Nicotiana debneyi gene for resistance to black root rot (Chalara elegans Nag Raj and Kendrick; Syn. Thielaviopsis basicola [Berk. and Broome] Ferraris), has been cloned and sequenced. The terminal 10 bases of this RAPD marker exactly match the sequence of the primer UBC418. UBC4181050 was restricted into 6 subfragments of 75, 91, 110, 174, 274, and 336 bp (the accession numbers for these 6 subfragments in the GenBank Data Base are U84217, U84218, U84219, U84220, U84221, and U84222, respectively) by combined restriction with HindIII and RsaI. The entire UBC4181050 marker and its 6 subfragments were used as probes in RFLP analyses. The RFLP analyses were performed on 'Delgold' tobacco (Nicotiana tabacum L.), the tobacco plants (2n = 48) recovered from the somatic hybrids between 'Delgold' and N. debneyi, the 'Delgold' tobacco translocation line carrying the N. debneyi gene for resistance to black root rot, the 'Delgold' tobacco addition lines carrying various N. debneyi chromosomes, and an additional 27 species in the genus Nicotiana. The analyses indicated that UBC4181050 is composed of at least 6 different repetitive DNA elements that are independently interspersed in N. debneyi genomes. Three of them (subfragments of 91, 274, and 336 bp) are N. debneyi specific. The N. debneyi specific repetitive DNA elements are distributed primarily within the taxonomic section (Suaveolentes) to which N. debneyi belongs.Key words: RAPD, repetitive DNA, Nicotiana debneyi, species-specific.

scholarly journals Application of Bacillus pumilus isolates for management of black rot disease in strawberry

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Farid Abd-El-Kareem ◽  
Ibrahim E. Elshahawy ◽  
Mahfouz M. M. Abd-Elgawad
Keyword(s):  
Root Rot ◽  
Bacillus Pumilus ◽  
Field Conditions ◽  
Black Rot ◽  
Bacterial Isolates ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Growth Area ◽  
Rot Disease ◽  
The Impact

Abstract Background Black root rot of strawberry plants caused by Rhizoctonia solani, Fusarium solani, and Pythium sp. is a serious disease in Egypt. Biocontrol agents have frequently proved to possess paramount and safe tools against many diseases. The impact of soil treatments with 3 Bacillus pumilus isolates on black root rot disease of strawberry plants caused by R. solani, F., and Pythium sp. under laboratory and field conditions was examined herein on the commonly used ‘Festival’ strawberry cultivar. To increase the bacterial adhesion and distribution on the roots, each seedling was dipped in bacterial cell suspension at 1 × 108 colony-forming units/ml of each separate bacterial isolate for 30 min then mixed with 5% Arabic gum. Results The tested B. pumilus isolates significantly reduced the growth area of these 3 fungi. The two bacterial isolates Nos. 2 and 3 reduced the growth area by more than 85.2, 83.6, and 89.0% for R. solani, F. solani, and Pythium sp., respectively. Likewise, the 3 bacterial isolates significantly (P ≤ 0.05) inhibited the disease under field conditions. Isolates Nos. 2 and 3 suppressed the disease incidence by 64.4 and 68.9% and disease severity by 65.3 and 67.3%, respectively. The fungicide Actamyl had effect similar to that of the 2 isolates. B. pumilus isolates significantly enhanced growth parameters and yields of strawberry plants; isolates Nos. 2 and 3 raised the yield by 66.7 and 73.3%, respectively. Conclusions Bacillus pumilus isolates could effectively manage the black rot disease in strawberry herein. Due to the significant impact of the root rot disease on strawberry yield, B. pumilus should be further tested to manage the disease on strawberry on large scale in Egypt.

scholarly journals In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Alemayehu Dugassa ◽  
Tesfaye Alemu ◽  
Yitbarek Woldehawariat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Fluorescens ◽  
Faba Bean ◽  
Fusarium Solani ◽  
Root Rot ◽  
Mycelial Growth ◽  
Biocontrol Agents ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Rot Disease

Abstract Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

Post-plant strategies for management of black root rot-related decline of perennial strawberry fields

2018 ◽  
Vol 104 ◽  
pp. 78-85 ◽  
Author(s):  
Timothy D. Miles ◽  
Benjamin W. Glass ◽  
Roger W. Sysak ◽  
Annemiek C. Schilder
Keyword(s):  
Root Rot ◽  
Plant Strategies ◽  
Black Root Rot
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