Substrate-histochemical investigations and ultrahistochemical demonstrations of acid phosphatase in larval and prepupal salivary glands of Drosophila melanogaster

1974 ◽  
Vol 155 (1) ◽  
pp. 75-89 ◽  
Author(s):  
Brita von Gaudecker ◽  
Elke-Maria Schmale
Keyword(s):  
Drosophila Melanogaster ◽  
Acid Phosphatase ◽  
Salivary Glands

scholarly journals Ultrastructural histochemical localization of acid phosphatase in salivary glands of Drosophila melanogaster.

1976 ◽  
Vol 24 (2) ◽  
pp. 463-467 ◽  
Author(s):  
J Anastasia-Sawicki ◽  
R J MacIntyre
Keyword(s):  
Drosophila Melanogaster ◽  
Acid Phosphatase ◽  
Salivary Glands ◽  
Incubation Time ◽  
Reaction Medium ◽  
Histochemical Localization ◽  
Wild Type ◽  
Lead Phosphate ◽  
Final Reaction Product ◽  
Optimal Incubation

The ultrastructural histochemical localization of acid phosphatase in salivary glands of third instar larvae of Drosophila melanogaster has been studied. Using Gomori's lead phosphate method for acid phosphatase detection, the optimal incubation time in the reaction medium was determined to be 30 min. When glands having wild-type acid phosphatase activity are incubated for this time, deposition of the final reaction product is observed in essentially every lysosome and artifactual staining is minimal.

Studies on Nucleolar RNA Synthesis in Drosophila Melanogaster

1972 ◽  
Vol 11 (3) ◽  
pp. 689-697
Author(s):  
H. M. KRIDER ◽  
W. PLAUT
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
Rna Synthesis ◽  
First Generation ◽  
Nucleolus Organizer ◽  
Fifth Generation ◽  
Autoradiographic Analysis ◽  
Temporally Correlated ◽  
Nucleolar Rna ◽  
Nucleolus Organizers

The influence of conditions resulting in bobbed phenotypes on nucleolar RNA synthesis and the formation of constrictions at nucleolus organizers was examined in larval tissues of Drosophila melanogaster. By means of [3H]uridine incorporation and autoradiographic analysis, a mutation at the bobbed locus was shown to limit the rate of nucleolar RNA synthesis in salivary glands of XO larvae. The formation of constrictions at the organizer sites of a 4-nucleolus-organizer stock was monitored in dividing neuroblast cells stained with acridine orange. Loss of the ribosomal cistrons had been reported by other workers when such stocks were maintained for several generations. In the first generation in our work, constrictions were visible at only 2 of the 4 nucleolus organizers. This situation persisted until the fifth generation, when constrictions appeared at all 4 of the organizer sites. An increase in the rate of nucleolar RNA synthesis in the salivary glands was temporally correlated with the appearance of the extra constrictions. We interpret these observations to mean that 2 of the organizers of the 4-nucleolus-organizer stock were caused to function through the loss of ribosomal RNA cistrons; thus the functional status of an organizer would appear to be subject to control.

Recent findings in oogenesis of Drosophila melanogaster

Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 45-57
Author(s):  
F. Giorgi ◽  
J. Jacob
Keyword(s):  
Drosophila Melanogaster ◽  
Acid Phosphatase ◽  
Ovarian Development ◽  
Superficial Layer ◽  
Blood Proteins ◽  
Selective Hydrolysis ◽  
Internal Lining ◽  
Hydrolysis Of ◽  
Zinc Iodide ◽  
Yolk Platelet

The role played by the vitellogenic oocytes of Drosophila melanogaster in relation to the elaboration of material taken from the haemolymph is examined by ultrastructural cytochemistry. As revealed by the Gomori procedure, acid phosphatase occurs widely over the forming yolk platelets of the cortical and central ooplasm. A number of Golgi apparatuses in thecortical ooplasm are also positively stained with lead precipitates. With the proceeding of the ovarian development it becomes progressively more difficult to demonstrate cytochemically the enzyme over the yolk platelets. In stage 9–10 chambers the acid phosphatase is restricted to the so-called associated body, while the rest of the yolk platelet appears devoid of lead deposits. By using a osmium zinc iodide (OZ1) complex as a preferential staining method for the Golgi apparatus, it has been shown that, apart from the apparatus itself, a number of OZI deposits occur over the superficial layer of the forming yolk platelets. When mature yolk platelets are formed at later stages, the OZI deposits in the yolk platelets come to be restricted to the cap-like region of the superficial layer which contains the associated body. In vitellogenic oocytes, both the internal lining of the limiting membrane of the forming yolk platelets and the associated body of the mature yolk platelets react positively, to cytochemical methods to demonstrate carbohydrates. The present findings are interpreted as indicating the involvement of lysosomal enzymes in the process of maturation of the yolk material. The suggestion is also made that such an involvement is required to accomplish a selective hydrolysis of those blood proteins which have been taken in by vitellogenic oocytes along with yolk precursors.

Combined histochemical and x-ray microanalytical studies on the copper-accumulating granules in the mid-gut of larval Drosophila

1977 ◽  
Vol 26 (1) ◽  
pp. 201-215
Author(s):  
R.L. Tapp ◽  
A. Hockaday
Keyword(s):  
Drosophila Melanogaster ◽  
Acid Phosphatase ◽  
Energy Dispersive ◽  
X Ray ◽  
Gut Epithelium

The copper-containing granules in the mid-gut epithelium of larval Drosophila melanogaster were examined for acid phosphatase by combined histochemistry and energy-dispersive, X-ray microanalysis. After incubation, many of the granules were shown to contain simultaneously copper and sulphur (which are normal constituents), and lead and phosphorus (which are the detectable elements of the reaction product). Earlier work has been consolidated and extended and the evidence that the granules are formed as cytolysosomes is reviewed.

Phosphatase Activity of Drosophila Salivary Glands

1948 ◽  
Vol s3-89 (8) ◽  
pp. 415-419
Author(s):  
W. L. DOYLE
Keyword(s):  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Quantitative Determination ◽  
Salivary Glands ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
The State ◽  
Staining Methods

The phosphatases in the cytoplasm and nuclei of Drosophila salivary glands are better preserved by fixation in absolute acetone than in 85 per cent, alcohol. In whole glands there is relatively little extraction of the enzyme during assay. Phosphatase activity is more resistant to incubation at neutrality than at pH 8.6, but in this material there is sufficient residual enzymatic activity to permit redetermination of alkaline, neutral, or acid phosphatase activity by staining methods after an initial quantitative determination. The state of the membranes of the gland affects the penetration of the substrate sufficiently to limit the activities obtained.

20-OH-ecdysone swells nuclear volume by alkalinization in salivary glands of Drosophila melanogaster

1993 ◽  
Vol 274 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Stefan W�nsch ◽  
Stefan Schneider ◽  
Albrecht Schwab ◽  
Hans Oberleithner
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
Nuclear Volume
Sign in / Sign up

Export Citation Format

Share Document