Quantitative analyses of atrial myoendocrine cells and plasma atrial natriuretic peptides (ANP) of the rat with special reference to the twenty-four-hour variations in secretory granules and plasma ANP concentrations

1988 ◽  
Vol 254 (1) ◽  
Author(s):  
Tsuyoshi Watanabe ◽  
Yasuo Uchiyama
Keyword(s):  
Special Reference ◽  
Natriuretic Peptides ◽  
Secretory Granules ◽  
Atrial Natriuretic Peptides ◽  
Myoendocrine Cells ◽  
Quantitative Analyses ◽  
Atrial Natriuretic

scholarly journals An immunocytochemical study on co-localization of cathepsin B and atrial natriuretic peptides in secretory granules of atrial myoendocrine cells of rat heart.

1989 ◽  
Vol 37 (3) ◽  
pp. 347-351 ◽  
Author(s):  
T Watanabe ◽  
M Watanabe ◽  
Y Ishii ◽  
H Matsuba ◽  
S Kimura ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Cathepsin B ◽  
Rat Heart ◽  
Secretory Granules ◽  
Atrial Natriuretic Peptides ◽  
Thin Sections ◽  
Gold Particles ◽  
Atrial Tissue ◽  
Myoendocrine Cells ◽  
Atrial Natriuretic

To examine localization of cathepsin B, a representative lysosomal cysteine protease, in atrial myoendocrine cells of the rat heart, immunohistochemistry at the light and electron microscopic level was applied to the atrial tissue, using a monospecific antibody for rat liver cathepsin B. In serial semi-thin sections, immunoreactivity for cathepsin B and atrial natriuretic peptides (ANP) was detected in the para-nuclear region of atrial myoendocrine cells. Several large granules and many fine granules in the region of the cells were positively stained by the cathepsin B antibody. Gold particles indicating cathepsin B antigenicity labeled secretory granules in the cells, which were also labeled by those indicating ANP, using thin sections of the Lowicryl K4M-embedded material. Moreover, some granules labeled densely by immunogold particles for cathepsin B seemed to be lysosomes. By double immunostaining using thin sections of the Epon-embedded material, gold particles indicating cathepsin B and ANP antigenicities were co-localized in secretory granules of the cells. By enzyme assay, activity of cathepsin B was three times higher in atrial tissue than ventricular tissue. The results suggest that co-localization of cathepsin B and ANP in secretory granules is compatible with the possibility that cathepsin B participates in the maturation process of ANP.

Atrial natriuretic peptides negatively and positively modulate circulating endothelin in humans

Metabolism ◽  
1996 ◽  
Vol 45 (3) ◽  
pp. 315-319 ◽  
Author(s):  
David L. Vesely ◽  
Shirley Chiou ◽  
Margaret A. Douglass ◽  
Michael T. McCormick ◽  
George Rodriguez-Paz ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Atrial Natriuretic Peptides ◽  
Atrial Natriuretic
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