Severe damage of cultured vascular endothelial cell monolayer after simultaneous exposure to cadmium and lead

1995 ◽  
Vol 28 (2) ◽  
Author(s):  
T. Kaji ◽  
M. Suzuki ◽  
C. Yamamoto ◽  
A. Mishima ◽  
M. Sakamoto ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Monolayer ◽  
Severe Damage ◽  
Simultaneous Exposure ◽  
Cadmium And Lead

scholarly journals Metastatic tumor cell attachment and invasion assay utilizing vascular endothelial cell monolayers.

1982 ◽  
Vol 30 (3) ◽  
pp. 214-220 ◽  
Author(s):  
G L Nicolson
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Tumor Cell ◽  
Basal Lamina ◽  
Cell Monolayer ◽  
Vascular Endothelial Cell ◽  
Metastatic Tumor ◽  
Vascular Endothelial ◽  
Metastatic Tumor Cell

Two of the more important steps in blood-borne tumor metastasis are attachment of the circulating malignant cells to the vascular endothelium and subsequent extravasation or invasion out of the blood vessel. A model for this process has been developed using cultured monolayers of vascular endothelial cells that synthesize a basal lamina or extracellular matrix (Kramer and Nicolson, Proc Natl Acad Sci USA 76:504, 1979). We have used this model to study metastatic tumor cell-endothelial cell interactions such as attachment to endothelial cells and their subsequent retraction and exposure of endothelial basal lamina as well as the interactions of metastatic tumor cells with the basal lamina leading to invasion and solubilization of this extracellular matrix. Morphological, immunological, and enzymological analysis of these steps in the metastatic process can be obtained using the vascular endothelial cell monolayer model for attachment and invasion.

Tumor necrosis factor and interleukin 1 alpha increase vascular endothelial permeability

1989 ◽  
Vol 257 (6) ◽  
pp. L399-L410 ◽  
Author(s):  
J. A. Royall ◽  
R. L. Berkow ◽  
J. S. Beckman ◽  
M. K. Cunningham ◽  
S. Matalon ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Interleukin 1 ◽  
Endotoxic Shock ◽  
Cell Monolayer ◽  
Vascular Endothelial Cell ◽  
Cell Permeability ◽  
Vascular Endothelial ◽  
Endothelial Cell Permeability ◽  
Necrosis Factor

Endotoxic shock is associated with acute vascular endothelial injury resulting in edema. Tumor necrosis factor (TNF) and interleukin 1 (IL-1) are cytokines produced by endotoxin-stimulated mononuclear phagocytes that are potential mediators of endotoxic shock. In this study, we investigated the effects of TNF and IL-1 alpha on vascular endothelial cell permeability in vitro. The movement of radiolabeled macromolecules of different sizes (57Co-vitamin B12, 125I-cytochrome c, and 131I-albumin; 6.5-35A) across bovine aortic endothelial cell monolayers was measured after exposure to these cytokines. TNF induced a time- and dose-dependent increase in endothelial cell monolayer permeability that was enhanced in the presence of serum. The peak increase was noted after 12 h of incubation with less alteration of permeability with longer incubations. IL-1 alpha caused a similar time-dependent increase in endothelial cell monolayer permeability, but the peak effect of IL-1 alpha was seen after 24 h. Therefore the increased permeability seen with TNF cannot be explained by release of endogenous IL-1 alone. Neither TNF nor IL-1 alpha increased release of [14C]adenine, and the only effect on lactate dehydrogenase release was a small, but statistically significant, increase after 24 h of incubation. From these studies, we conclude that TNF and IL-1 alpha directly increase vascular endothelial cell permeability in vitro and speculate that these cytokines may be involved in the acute vascular endothelial injury associated with endotoxic shock.

Effect of Vascular Endothelial Growth Factor on Cultured Endothelial Cell Monolayer Transport Properties

2000 ◽  
Vol 59 (2) ◽  
pp. 265-277 ◽  
Author(s):  
Yong S. Chang ◽  
Lance L. Munn ◽  
Mechteld V. Hillsley ◽  
Randal O. Dull ◽  
Jin Yuan ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Transport Properties ◽  
Cell Monolayer ◽  
Vascular Endothelial ◽  
Endothelial Cell Monolayer ◽  
Endothelial Growth Factor
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