Mechanism of blue-light-induced plasma-membrane depolarization in etiolated cucumber hypocotyls

Planta ◽  
1992 ◽  
Vol 188 (2) ◽  
pp. 199-205 ◽  
Author(s):  
EdgarP. Spalding ◽  
DanielJ. Cosgrove
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Membrane Depolarization

Oxidative burst and NO generation as initial response to ischemia in flow-adapted endothelial cells

2001 ◽  
Vol 280 (5) ◽  
pp. H2126-H2135 ◽  
Author(s):  
Yefim Manevich ◽  
Abu Al-Mehdi ◽  
Vladimir Muzykantov ◽  
Aron B. Fisher
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Oxidative Burst ◽  
Initial Response ◽  
Membrane Depolarization ◽  
No Synthase ◽  
Potential Production ◽  
Perfusion Chamber ◽  
Intracellular Ca ◽  
Static Conditions

Shear stress modulates endothelial physiology, yet the effect(s) of flow cessation is poorly understood. The initial metabolic responses of flow-adapted bovine pulmonary artery endothelial cells to the abrupt cessation of flow (simulated ischemia) was evaluated using a perfusion chamber designed for continuous spectroscopy. Plasma membrane potential, production of reactive O2 species (ROS), and intracellular Ca2+ and nitric oxide (NO) levels were measured with fluorescent probes. Within 15 s after flow cessation, flow-adapted cells, but not cells cultured under static conditions, showed plasma membrane depolarization and an oxidative burst with generation of ROS that was inhibited by diphenyleneiodonium. EGTA-inhibitable elevation of intracellular Ca2+ and NO were observed at ∼30 and 60 s after flow cessation, respectively. NO generation was decreased in the presence of inhibitors of NO synthase and calmodulin. Thus flow-adapted endothelial cells sense the altered hemodynamics associated with flow cessation and respond by plasma membrane depolarization, activation of NADPH oxidase, Ca2+ influx, and activation of Ca2+/calmodulin-dependent NO synthase. This signaling response is unrelated to cellular anoxia.

scholarly journals Immunohistochemical Detection of Blue Light-Induced Phosphorylation of the Plasma Membrane H+-ATPase in Stomatal Guard Cells

2011 ◽  
Vol 52 (7) ◽  
pp. 1238-1248 ◽  
Author(s):  
Maki Hayashi ◽  
Shin-ichiro Inoue ◽  
Koji Takahashi ◽  
Toshinori Kinoshita
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Guard Cells ◽  
Immunohistochemical Detection ◽  
Stomatal Guard Cells

scholarly journals Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation in Arabidopsis

2020 ◽  
Vol 19 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Maki Hayashi ◽  
Hodaka Sugimoto ◽  
Hirotaka Takahashi ◽  
Motoaki Seki ◽  
Kazuo Shinozaki ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Guard Cells ◽  
Stomatal Opening

Raf-like kinases CBC1 and CBC2 negatively regulate phosphorylation of plasma membrane H+-ATPase in guard cells and blue light-dependent stomatal opening.

BLUE LIGHT PERCEPTION BY ENDOGENOUS REDOX COMPONENTS OF THE PLANT PLASMA MEMBRANE

1995 ◽  
Vol 61 (5) ◽  
pp. 518-522 ◽  
Author(s):  
Han Asard ◽  
Nele Horemans ◽  
Winslow R. Briggs ◽  
Roland J. Caubergs
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Light Perception ◽  
Plant Plasma Membrane
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