scholarly journals Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation in Arabidopsis

2020 ◽  
Vol 19 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Maki Hayashi ◽  
Hodaka Sugimoto ◽  
Hirotaka Takahashi ◽  
Motoaki Seki ◽  
Kazuo Shinozaki ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Guard Cells ◽  
Stomatal Opening

Raf-like kinases CBC1 and CBC2 negatively regulate phosphorylation of plasma membrane H+-ATPase in guard cells and blue light-dependent stomatal opening.

scholarly journals Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen

2021 ◽  
Vol 12 ◽  
Author(s):  
Shigeo Toh ◽  
Naoki Takata ◽  
Eigo Ando ◽  
Yosuke Toda ◽  
Yin Wang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Transgenic Plants ◽  
Woody Plants ◽  
Stem Elongation ◽  
Guard Cells ◽  
Gus Expression ◽  
Stomatal Opening ◽  
Wild Type ◽  
Exchange System ◽  
Gas Exchange System

Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.

scholarly journals Immunohistochemical Detection of Blue Light-Induced Phosphorylation of the Plasma Membrane H+-ATPase in Stomatal Guard Cells

2011 ◽  
Vol 52 (7) ◽  
pp. 1238-1248 ◽  
Author(s):  
Maki Hayashi ◽  
Shin-ichiro Inoue ◽  
Koji Takahashi ◽  
Toshinori Kinoshita
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Guard Cells ◽  
Immunohistochemical Detection ◽  
Stomatal Guard Cells

scholarly journals Promotion and Upregulation of a Plasma Membrane Proton-ATPase Strategy: Principles and Applications

2021 ◽  
Vol 12 ◽  
Author(s):  
Zirong Ren ◽  
Bazhen Suolang ◽  
Tadashi Fujiwara ◽  
Dan Yang ◽  
Yusuke Saijo ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plant Growth ◽  
Guard Cells ◽  
Stomatal Opening ◽  
Limiting Factor ◽  
Leaf Photosynthesis ◽  
Proton Atpase ◽  
Potential Applications ◽  
Stomatal Guard Cells

Plasma membrane proton-ATPase (PM H+-ATPase) is a primary H+ transporter that consumes ATP in vivo and is a limiting factor in the blue light-induced stomatal opening signaling pathway. It was recently reported that manipulation of PM H+-ATPase in stomatal guard cells and other tissues greatly improved leaf photosynthesis and plant growth. In this report, we review and discuss the function of PM H+-ATPase in the context of the promotion and upregulation H+-ATPase strategy, including associated principles pertaining to enhanced stomatal opening, environmental plasticity, and potential applications in crops and nanotechnology. We highlight the great potential of the promotion and upregulation H+-ATPase strategy, and explain why it may be applied in many crops in the future.

scholarly journals K+ transport properties of K+ channels in the plasma membrane of Vicia faba guard cells.

1988 ◽  
Vol 92 (5) ◽  
pp. 667-683 ◽  
Author(s):  
J I Schroeder
Keyword(s):  
Plasma Membrane ◽  
Transport Properties ◽  
Vicia Faba ◽  
Guard Cell ◽  
Guard Cells ◽  
Stomatal Opening ◽  
Whole Cell ◽  
K Channels ◽  
K Transport ◽  
K Currents

Electrical properties of the plasma membrane of guard cell protoplasts isolated from stomates of Vicia faba leaves were studied by application of the whole-cell configuration of the patch-clamp technique. The two types of K+ currents that have recently been identified in guard cells may allow efflux of K+ during stomatal closing, and uptake of K+ during stomatal opening (Schroeder et al., 1987). A detailed characterization of ion transport properties of the inward-rectifying (IK+,in) and the outward-rectifying (IK+,out) K+ conductance is presented here. The permeability ratios of IK+,in and IK+,out currents for K+ over monovalent alkali metal ions were determined. The resulting permeability sequences (PK+ greater than PRb+ greater than PNa+ greater than PLi+ much greater than PCs+) corresponded closely to the ion specificity of guard cell movements in V. faba. Neither K+ currents exhibited significant inactivation when K+ channels were activated for prolonged periods (greater than 10 min). The absence of inactivation may permit long durations of K+ fluxes, which occur during guard cell movements. Activation potentials of inward K+ currents were not shifted when external K+ concentrations were changed. This differs strongly from the behavior of inward-rectifying K+ channels in animal tissue. Blue light and fusicoccin induce hyperpolarization by stimulation of an electrogenic pump. From slow-whole-cell recordings it was concluded that electrogenic pumps require cytoplasmic substrates for full activation and that the magnitude of the pump current is sufficient to drive K+ uptake through IK+,in channels. First, direct evidence was gained for the hypothesis that IK+,in channels are a molecular pathway for K+ accumulation by the finding that IK+,in was blocked by Al3+ ions, which are known to inhibit stomatal opening but not closing. The results presented in this study strongly support a prominent role for IK+,in and IK+,out channels in K+ transport across the plasma membrane of guard cells.

scholarly journals Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth

2013 ◽  
Vol 111 (1) ◽  
pp. 533-538 ◽  
Author(s):  
Y. Wang ◽  
K. Noguchi ◽  
N. Ono ◽  
S.-i. Inoue ◽  
I. Terashima ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plant Growth ◽  
Guard Cells ◽  
Stomatal Opening

scholarly journals The Plasma Membrane H+-ATPase AHA1 Plays a Major Role in Stomatal Opening in Response to Blue Light

2016 ◽  
Vol 171 (4) ◽  
pp. 2731-2743 ◽  
Author(s):  
Shota Yamauchi ◽  
Atsushi Takemiya ◽  
Tomoaki Sakamoto ◽  
Tetsuya Kurata ◽  
Toshifumi Tsutsumi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Stomatal Opening

scholarly journals Blue Light Regulation of Stomatal Opening and the Plasma Membrane H+-ATPase

2017 ◽  
Vol 174 (2) ◽  
pp. 531-538 ◽  
Author(s):  
Shin-ichiro Inoue ◽  
Toshinori Kinoshita
Keyword(s):  
Plasma Membrane ◽  
Blue Light ◽  
Light Regulation ◽  
Stomatal Opening
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