Immuno-electron-microscopic study of the prolactin cells in the pituitary gland of male Wistar rats during aging

1988 ◽  
Vol 251 (2) ◽  
pp. 353-358 ◽  
Author(s):  
Leonie J. A. van Putten ◽  
Amanda J. Kiliaan
Author(s):  
Hepei Liu ◽  
Yanming Hou ◽  
Xiaofeng Chang ◽  
Shulan Wang

Johnson stated that man has always been interested in the structure of things in the world around him. In this regard, the electron microscopy, a modern technique, is used for research in traditional Chinese medicine. The terms and theories of TCM are quite different from those of Western medicine. The theory of TCM indicates that pi (spleen in TCM) has important roles in human health. According to the basic theory of TCM, one such function of the pi is nourishing the muscles. A significant decrease in the number of mitochondria with normal structure and a significant increase in the number of mitochondria with abnormal structure in skeletal muscle of piqixuzheng (spleen energy deficiency in TCM) rats have been reported. The present work is a quantitative electron microscopic study on mitochondria of smooth muscle in piqixuzheng rats.Male Wistar rats were used in this study. The piqixuzheng rats were produced by treatment with houpo (Magnolia officinalis), Zhishi(Fructus aurantii immaturus), dahuang (Radix et rhizoma) and fasting day other day for six weeks.


1989 ◽  
Vol 258 (3) ◽  
Author(s):  
Haruo Nogami ◽  
Kaoru Suzuki ◽  
Kazutaka Matsui ◽  
Shinichi Ookuma ◽  
Hiroshi Ishikawa

Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.


Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.


Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.


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