Class I major histocompatibility complex cDNA clones from sheep thymus: alternative splicing could make a long cytoplasmic tail

1990 ◽  
Vol 32 (2) ◽  
Author(s):  
Dario Grossberger ◽  
Wayne Hein ◽  
Anne Marcuz
Keyword(s):  
Major Histocompatibility Complex ◽  
Alternative Splicing ◽  
Cytoplasmic Tail ◽  
Class I ◽  
Cdna Clones ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Sheep Thymus

scholarly journals Calnexin retains unassembled major histocompatibility complex class I free heavy chains in the endoplasmic reticulum.

1994 ◽  
Vol 180 (1) ◽  
pp. 407-412 ◽  
Author(s):  
S Rajagopalan ◽  
M B Brenner
Keyword(s):  
Endoplasmic Reticulum ◽  
Major Histocompatibility Complex ◽  
Cell Surface ◽  
Mhc Class I ◽  
Cytoplasmic Tail ◽  
Class I ◽  
Major Histocompatibility ◽  
Stable Transfectants ◽  
Histocompatibility Complex ◽  
Er Retention

The assembly of major histocompatibility complex (MHC) class I molecules involves the association of heavy (H) chain with beta 2-microglobulin (beta 2m) and peptide. Unassembled class I H chains do not exit the endoplasmic reticulum (ER) and this is exemplified by the beta 2m-deficient human melanoma FO-1 where free class I H chains are unable to complete assembly. In pulse chase experiments involving FO-1 cells, unassembled free class I H chains were shown to be stably associated with calnexin (IP90/p88), a 90-kD integral membrane molecular chaperone of the ER. To establish a role for calnexin in mediating this retention, we transfected FO-1 cells with a cytoplasmic tail deletion mutant of calnexin. Since the cytoplasmic tail contains the ER retention motif, these mutant calnexin molecules leave the ER and progress to the cell surface. In these stable transfectants of FO-1, free class I H chains also exited the ER and trafficked to the cell surface with calnexin, thus establishing a role for calnexin in the quality control of MHC class I assembly through mediating the ER retention of incompletely assembled class I H chains.

Signals controlling alternative splicing of major histocompatibility complex H-2 class I pre-mRNA

1988 ◽  
Vol 28 (2) ◽  
pp. 81-90 ◽  
Author(s):  
Diane E. Handy ◽  
James McCluskey ◽  
Andrew M. Lew ◽  
John E. Coligan ◽  
David H. Margulies
Keyword(s):  
Major Histocompatibility Complex ◽  
Alternative Splicing ◽  
Class I ◽  
Major Histocompatibility ◽  
Histocompatibility Complex
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