Immunohistological evidence of methionine enkephalin-like material in the brain of the migratory locust

1981 ◽  
Vol 218 (2) ◽  
Author(s):  
Christian R�my ◽  
MauriceP. Dubois
Keyword(s):  
Migratory Locust ◽  
Methionine Enkephalin ◽  
The Brain

NPY-like peptides occur in the nervous system and midgut of the migratory locust, Locusta migratoria and in the brain of the grey fleshfly, Sarcophaga bullata

Peptides ◽  
1988 ◽  
Vol 9 (5) ◽  
pp. 1027-1036 ◽  
Author(s):  
L. Schoofs ◽  
J.M. Danger ◽  
S. Jegou ◽  
G. Pelletier ◽  
R. Huybrechts ◽  
...  
Keyword(s):  
Nervous System ◽  
Locusta Migratoria ◽  
Migratory Locust ◽  
Sarcophaga Bullata ◽  
The Brain

scholarly journals HPLC/RIA Analysis of Biologically Active Peptide, Methionine Enkephalin, in the Brain of Seizure-Susceptible El Mouse.

1991 ◽  
Vol 9 (2) ◽  
pp. 169-172
Author(s):  
Seiji Koide ◽  
Hiroshi Onishi ◽  
Masafumi Katayama ◽  
Sakae Yamagami ◽  
Yukio Kawakita
Keyword(s):  
Biologically Active ◽  
El Mouse ◽  
Methionine Enkephalin ◽  
Active Peptide ◽  
Biologically Active Peptide ◽  
The Brain

Carrier-mediated transport of enkephalins and N-Tyr-MIF-1 across blood-brain barrier

1986 ◽  
Vol 251 (4) ◽  
pp. E477-E482 ◽  
Author(s):  
W. A. Banks ◽  
A. J. Kastin ◽  
A. J. Fischman ◽  
D. H. Coy ◽  
S. L. Strauss
Keyword(s):  
Central Nervous System ◽  
Half Time ◽  
Small Peptides ◽  
Methionine Enkephalin ◽  
Carrier Mediated Transport ◽  
Sodium Dependent ◽  
Residual Radioactivity ◽  
The Central Nervous System ◽  
Blood Transport ◽  
The Brain

The saturable, carrier-mediated system capable of the brain-to-blood transport of small peptides with an N-terminal tyrosine was characterized. The rate of disappearance of intraventricularly injected iodinated peptide in the presence or absence of the inhibitor being tested was determined from formulas based on the residual radioactivity in the brains of mice after decapitation. The injection of 100 nmol/mouse of unlabeled N-Tyr-MIF-1 (TMIF) increased the half-time disappearance of 125I-TMIF (ITMIF) in the central nervous system (CNS) from 14.1 to 88.7 min (P less than 0.00005). Technetium, a substance transported out of the brain by the same system that transports iodine, was used as a control; the half-time disappearance of technetium pertechnetate was unaffected by unlabeled TMIF. With two related but distinct techniques, the maximum transport rate out of the CNS (Vmax) for TMIF was 0.266 nmol X g of brain per min (method 1) and 0.297 nmol X g-1 X min-1 (method 2), while the amount of unlabeled material needed to achieve 50% of Vmax (Km) was 15.2 nmol/g (method 1) and 15.1 nmol/g (method 2). The lack of effect of the tyrosinated fragments of TMIF as inhibitors indicates that TMIF is being transported in intact form. The Vmax for methionine enkephalin determined with labeled and unlabeled methionine enkephalin was 0.630 nmol X g-1 X min-1 and the Km was 24.95 nmol/g. Studies with the metabolic modulators furosemide, acetozolamide, reserpine, ouabain, and theophylline suggest that the system is sodium dependent and probably independent of ATPase.(ABSTRACT TRUNCATED AT 250 WORDS)

Peptides with Morphine-like Action in the Brain

1977 ◽  
Vol 130 (3) ◽  
pp. 298-304 ◽  
Author(s):  
Hans W. Kosterlitz ◽  
John Hughes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Opioid Peptides ◽  
Endogenous Opioid Peptides ◽  
Endogenous Opioid ◽  
Methionine Enkephalin ◽  
Therapeutic Implications ◽  
The Brain ◽  
Development Of Tolerance

SummaryThe reasons which led to the search in the brain for substances with morphine-like actions are discussed. Two pentapeptides, methionine-enkephalin and leucine-enkephalin, were isolated. The amino acid sequence of methionine-enkephalin occurs also in the pituitary prohormone β-lipotropin, of which longer fragments (endorphins) of up to 31 amino acids exhibit strong morphine-like action.The physiological significance of these short and long opioid peptides is discussed, particularly with regard to their possible roles as neurotransmitter or neuromodulator.With regard to the mechanisms involved in the development of tolerance to and dependence on opiates, the importance of interaction between the endogenous opioid peptides and the exogenous opiate alkaloids is stressed.The possible therapeutic implications are discussed briefly.

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