Immunohistochemical localization of polypeptide hormones in endocrine cells of the digestive tract of Branchiostoma lanceolatum

1981 ◽  
Vol 219 (3) ◽  
Author(s):  
M. Reinecke
1991 ◽  
Vol 91 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Dietrich W. Scheuermann ◽  
Dirk Adriaensen ◽  
Jean-Pierre Timmermans ◽  
Marie H.A. De Groodt-Lasseel

Development ◽  
1984 ◽  
Vol 82 (1) ◽  
pp. 131-145
Author(s):  
B. B. Rawdon ◽  
Beverley Kramer ◽  
Ann Andrew

The aim of this experiment was to find out whether or not, at early stages of development, progenitors of the various types of gut endocrine cells are localized to one or more specific regions of the gastrointestinal tract. Transverse strips of blastoderm two to four somites in length were excised between the levels of somites 5 and 27 in chick embryos at 5- to 24-somite stages and were cultured as chorioallantoic grafts. The distribution of endocrine cells in the grafts revealed confined localization of progenitor cells only in the case of insulinimmunoreactive cells. Theprogenitors of cells with somatostatin-, pancreatic polypeptide-, glucagon-, secretin-, gastrin/CCK-, motilin-, neurotensin- and serotonin-like immunoreactivity were distributed along the length of the presumptive gut at the time of explantation; indeed, in many cases they were more widespread than are their differentiated progeny in normal gut of the same age. This finding indicates that conditions in grafts must differ from those that operate in the intact embryo. Also it may explain the occurrence of ectopic gut or pancreatic endocrine cells in tumours of the digestive tract.


1999 ◽  
Vol 434 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Stefano La Rosa ◽  
Silvia Uccella ◽  
Paola Billo ◽  
Carla Facco ◽  
Fausto Sessa ◽  
...  

1994 ◽  
Vol 277 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Loredana D'Este ◽  
Roberto Buffa ◽  
Micaela Pelagi ◽  
Antonio G. Siccardi ◽  
Tindaro Renda

2014 ◽  
Vol 46 (5) ◽  
pp. 343-351 ◽  
Author(s):  
Huan Wang ◽  
Shengzhou Zhang ◽  
Naizhen Zhou ◽  
Chaolin Wang ◽  
Xiaobing Wu

2008 ◽  
Vol 110 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Clarice Machado Dos Santos ◽  
Aparecida Alves Do Nascimento ◽  
Adriano Lucio Peracchi ◽  
Daniela Dias ◽  
Thatiana Paz Ribeiro ◽  
...  

1993 ◽  
Vol 41 (11) ◽  
pp. 1617-1622 ◽  
Author(s):  
A Martínez ◽  
M A Burrell ◽  
M Kuijk ◽  
L M Montuenga ◽  
A Treston ◽  
...  

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.


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