An immunohistochemical analysis of the ontogeny, distribution and coexistence of 12 regulatory peptides and serotonin in endocrine cells and nerve fibers of the digestive tract of the turbot, Scophthalmus maximus (Teleostei)

1996 ◽  
Vol 195 (1) ◽  
pp. 87-101 ◽  
Author(s):  
M. Reinecke ◽  
C. Müller ◽  
H. Segner
Keyword(s):  
Digestive Tract ◽  
Endocrine Cells ◽  
Immunohistochemical Analysis ◽  
Regulatory Peptides ◽  
Scophthalmus Maximus ◽  
Nerve Fibers ◽  
Turbot Scophthalmus Maximus

scholarly journals Localization of amidating enzymes (PAM) in rat gastrointestinal tract.

1993 ◽  
Vol 41 (11) ◽  
pp. 1617-1622 ◽  
Author(s):  
A Martínez ◽  
M A Burrell ◽  
M Kuijk ◽  
L M Montuenga ◽  
A Treston ◽  
...  
Keyword(s):  
Small Intestine ◽  
In Situ Hybridization ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Endocrine Cells ◽  
Regulatory Peptides ◽  
Serial Sections ◽  
Gastric Glands ◽  
Amidating Enzymes

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.

Hemolysis of turbot(Scophthalmus maximus)blood cell with Quillaja saponaria saponin

2013 ◽  
Vol 37 (7) ◽  
pp. 1073
Author(s):  
Yujuan WANG ◽  
Xiuhua WANG ◽  
Wen HAN ◽  
Rui WANG ◽  
Jie HUANG
Keyword(s):  
Blood Cell ◽  
Scophthalmus Maximus ◽  
Quillaja Saponaria ◽  
Turbot Scophthalmus Maximus
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