Immune reaction of tumor-bearing mice to Propionibacterium acnes and the antitumor effect of the bacteria

1982 ◽  
Vol 14 (1) ◽  
Author(s):  
V. Silobrcic ◽  
G. Fredrickson ◽  
R. Sedlacek ◽  
H.D. Suit ◽  
G. Wolberg
Keyword(s):  
Antitumor Effect ◽  
Propionibacterium Acnes ◽  
Immune Reaction ◽  
Tumor Bearing

scholarly journals Antitumor activity of a fungal glucan tylopilan and Propionibacterium acnes preparation

2014 ◽  
Vol 63 (3-4) ◽  
pp. 293-298 ◽  
Author(s):  
Jan Grzybek ◽  
Izabella Zgórniak-Nowosielska ◽  
Andrzej Kasprowicz ◽  
Barbara Zawilińska ◽  
Stanisław Kohlmunzer
Keyword(s):  
Immune Response ◽  
Antitumor Activity ◽  
Antitumor Effect ◽  
Propionibacterium Acnes ◽  
Single Injection ◽  
Fruiting Bodies ◽  
Mean Survival Time ◽  
Tumor Bearing ◽  
Cell Inoculation

The study evaluated the antitumor activity of tylopilan, aβ- (1→3) (1→6) linked glucan isolated from fruiting bodies of <i>Tylopilus felleus</i> (Bull.: Fr.) P. Karst. (<i>Boletaceae</i>), and <i>Propionibacterium acnes</i> (<i>P.a.</i>) preparation. The antitumor effect of tylopilan and <i>P.a.</i> used alone or in combination was studied in NMRI mice inoculated i.p. with 106 180-TG Crocker tumor cells. All experiments were based on a pretreatment with tylopilan and/or <i>P.a.</i> 5 days and/or 2 h before tumor cell inoculation. Mean survival time (MST) of tumor - bearing mice was significantly prolonged in comparison to control mice by a single injection of tylopilan (25 µg/mouse or 50 µg/mouse) or <i>P.a.</i> (1 mg/mouse). MST was 23.6; 22.8 days in the tylopilan injected mice and 17.5 in the control animals. Tylopilan injected in conjunction with <i>P.a.</i> prolonged signifi-cantly MST in comparison to control mice as well as to tylopilan alone treated mice. We have found that <i>P.a.</i> which stimulate immune response enhanced significantly antitumor activity of tylopilan. The cytotoxicity of tylopilan at concentrations of 300, 150, 75 and 37.5 µg/ml towards 180-TG Crocker cells in vitro studies was evaluated. All examined tylopilan concentrations showed cytotoxic activity.

Quercetin enhances the antitumor effect of trichostatin A and suppresses muscle wasting in tumor-bearing mice

2018 ◽  
Vol 9 (2) ◽  
pp. 871-879 ◽  
Author(s):  
Shu-Ting Chan ◽  
Cheng-Hung Chuang ◽  
Yi-Chin Lin ◽  
Jiunn-Wang Liao ◽  
Chong-Kuei Lii ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Antitumor Effect ◽  
Muscle Wasting ◽  
Muscle Protein ◽  
Trichostatin A ◽  
Tumor Bearing ◽  
Muscle Protein Degradation

Quercetin prevents TSA-induced muscle wasting by down-regulating FOXO1 mediated muscle protein degradation.

Preparation of enzymatically highly active pegylated-D-amino acid oxidase and its application to antitumor therapy

2021 ◽  
Vol 18 ◽  
Author(s):  
Hideaki Nakamura ◽  
Appiah Enoch ◽  
Shotaro Iwaya ◽  
Sakura Furusho ◽  
Shoko Tsunoda ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antitumor Effect ◽  
Tumor Tissue ◽  
Acid Oxidase ◽  
Antitumor Therapy ◽  
Amino Acid Oxidase ◽  
Tumor Bearing ◽  
Highly Active ◽  
High Enzyme ◽  
High Enzyme Activity

Background: D-amino acid oxidase (DAO) is an H2O2-generating enzyme, and tumor growth suppression by selective delivery of porcine DAO in tumors via the cytotoxic action of H2O2 has been reported. DAO isolated from Fusarium spp. (fDAO) shows much higher enzyme activity than porcine DAO, although the application of fDAO for antitumor treatment has not yet been determined. Objective: The purpose of this study was to prepare enzymatically highly active pegylated-fDAO, and to determine whether it accumulates in tumors and exerts a potent antitumor effect in tumor-bearing mice. Methods: Polyethylene glycol (PEG; Mw. 2000) was conjugated to fDAO to form PEGylated fDAO (PEG-fDAO). PEGfDAO was intravenously administered into S180 tumor-bearing mice, and the body distribution and antitumor activity of PEG-fDAO was determined. Results: The enzyme activity of PEG-fDAO was 26.1 U/mg, which was comparable to that of fDAO. Intravenously administered PEG-fDAO accumulated in tumors with less distribution in normal tissue except in the plasma. Enzyme activity in the tumor was 60–120 mU/g-tissue over 7–20 h after i.v. injection of 0.1 mg of PEG-fDAO. To generate the H2O2 in the tumor tissue, PEG-fDAO was intravenously administered, and then, D-phenylalanine was intraperitoneally administered after a lag time. No remarkable tumor suppression effect was observed under conditions used in this study, compared to the non-treated group. Conclusion: The results suggest that PEG-fDAO maintained high enzymatic activity after pegylation. Treatment with PEGfDAO conferred high enzyme activity on tumor tissue; 3–6 fold higher than that of previously reported pDAO; however, high enzyme activity in the plasma limited repeated treatment owing to lethal toxicity, which seemingly led to poor therapeutic outcome. Overall, the use of PEG-fDAO is promising for antitumor therapy, although the suppression of DAO activity in the plasma would also be required rather than only the increase in DAO activity in the tumor for an antitumor effect.

Polysaccharide from Eucheuma Gelatinae Enhance Immunocompetence against Murine H22 Tumor In Vivo

2014 ◽  
Vol 989-994 ◽  
pp. 1056-1059
Author(s):  
Juan Tang ◽  
Zhen Kong ◽  
Dong Yue Liu ◽  
An Jun Liu
Keyword(s):  
Flow Cytometry ◽  
Clinical Trials ◽  
Antitumor Effect ◽  
Biological Activities ◽  
Tumor Bearing

New antitumor strategies are underway and play important roles in clinical trials for combating cancer in future. Eucheuma gelatinae contains a certain amount of polysaccharides, which has various biological activities. In this study, the antitumor effect of Eucheuma gelatinae polysaccharide on murine H22 tumor bearing mice has been investigated. Histological stain, flow cytometry and other methods are applied to evaluate the effects of Eucheuma gelatinae polysaccharide on immunocompetence in vivo. The data indicates that Eucheuma gelatinae polysaccharide has antitumor effect in vivo by enhancing immunocompetence of the tumor bearers.

scholarly journals Antitumor effects of melanin fromLachnumYM226 and its derivative in H22 tumor-bearing mice

MedChemComm ◽  
2018 ◽  
Vol 9 (6) ◽  
pp. 1059-1068 ◽  
Author(s):  
Fang Shi ◽  
Jinglei Li ◽  
Ziyang Ye ◽  
Liuqing Yang ◽  
Tingting Chen ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
Antitumor Effects ◽  
Tumor Bearing

LM and ALM showed notable antitumor effect in H22 tumor-bearing mice and ALM was more effective.

scholarly journals Gan-Qing-Ning Formula Inhibits the Growth of Hepatocellular Carcinoma by Promoting Apoptosis and Inhibiting Angiogenesis in H22 Tumor-Bearing Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Fan-Yan Zeng ◽  
Kai-Li Zhao ◽  
Le-Zhen Lin ◽  
Ying Deng ◽  
Si Qin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antitumor Effect ◽  
Caspase 3 ◽  
Tumor Tissue ◽  
Mitochondrial Apoptosis ◽  
Apoptosis Pathway ◽  
Expression Levels ◽  
Mitochondrial Apoptosis Pathway ◽  
Tumor Bearing ◽  
Mrna Expression Levels

Objective. Gang-Qing-Ning (GQN) is a traditional Chinese medicine formula that has been used in the treatment of hepatocellular carcinoma (HCC) in the folk population for decades. However, scientific validation is still necessary to lend credibility to the traditional use of GQN against HCC. This study investigates the antitumor effect of GQN on H22 tumor-bearing mice and its possible mechanism. Methods. Fifty H22 tumor-bearing mice were randomly assigned to five groups. Three groups were treated with high, medium, and low dosages of GQN (27.68, 13.84, and 6.92 g/kg, respectively); the positive control group was treated with cytoxan (CTX) (20 mg/kg) and the model group was treated with normal saline. After 10 days’ treatment, the tumor inhibitory rates were calculated. Pathological changes in tumor tissue were observed, and the key proteins and genes of the mitochondrial apoptosis pathway were measured, as well as the mRNA expression levels of VEGF in tumor tissue. Results. The tumor inhibitory rates of high, medium, and low dosages of GQN groups were 47.39%, 38.26%, and 22.17%, respectively. The high dosage of the GQN group significantly increased the protein and mRNA expression levels of Bax, Cyt-C, and cleaved Caspase 3 (or Caspase 3) (P<0.01) but decreased the expression levels of Bcl-2, VEGF, and microvessel density (MVD) (P<0.01). Conclusions. The high dosage of GQN can significantly inhibit the tumor growth in H22 tumor-bearing mice. It exerts the antitumor effect by enhancing proapoptotic factors and inhibiting the antiapoptotic factor of the mitochondrial apoptosis pathway and inhibiting tumor angiogenesis.

scholarly journals Rebamipide does not interfere with the antitumor effect of radiotherapy or chemotherapy in human oral tumor-bearing nude mice

2015 ◽  
Vol 129 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Masafumi Shibamori ◽  
Masayuki Sato ◽  
Naoya Uematsu ◽  
Takako Nakashima ◽  
Asuka Sato ◽  
...  
Keyword(s):  
Nude Mice ◽  
Antitumor Effect ◽  
Tumor Bearing ◽  
Oral Tumor
Sign in / Sign up

Export Citation Format

Share Document