Copper induced metabolic changes in Sunetta scripta (Bivalvia): Oxygen uptake and lactic acid production

1992 ◽  
Vol 48 (4) ◽  
Author(s):  
ClaraMargret Katticaran ◽  
K.Y.Mohammed Salih
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Metabolic Changes

Biochemical appraisal of a milk diluent for semen

1960 ◽  
Vol 54 (2) ◽  
pp. 166-169
Author(s):  
K. Friis Jakobsen ◽  
T. Mann
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Incubation Period ◽  
Milk Fat ◽  
Acid Production ◽  
Marked Effect ◽  
Lactic Acid Production ◽  
Milk Product ◽  
Bull Semen ◽  
Boar Spermatozoa

1. A study was made of the effects of a milk diluent on bull, ram and boar spermatozoa. Respiration and fructolysis of spermatozoa were used as the two main criteria of sperm activity. The milk diluent was a standardized and commercially available milk product, consisting of sterilized and homogenized milk, supplemented with milk fat.2. The rate of oxygen uptake measured manometrically in the presence of air was increased by the addition of the milk diluent throughout the entire incubation period. Fructose utilization was assessed by the rate of lactic-acid production. The rate of the anaerobic lactic-acid formation was higher in the presence of the milk diluent during the later stages of incubation.3. The effect of the milk diluent on sperm respiration was most striking in experiments with the sperm-rich portion of boar ejaculate obtained by fractionated collection. A somewhat less marked effect was observed with bull semen, and in ram semen the effect was comparatively weak.

The Effect of Washing on the Motility and Metabolism of Ram, Bull and Rabbit Spermatozoa

1953 ◽  
Vol 30 (2) ◽  
pp. 200-213
Author(s):  
I G. WHITE
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Acid Production ◽  
Bacterial Contamination ◽  
Aerobic Glycolysis ◽  
Lactic Acid Production ◽  
Total Oxygen ◽  
The Mean ◽  
Almost All ◽  
Rabbit Spermatozoa

1. The motility, oxygen uptake and aerobic glycolysis of unwashed, once- and twice-washed ram, bull and rabbit spermatozoa have been studied in a sodium phosphate-fructose diluent over a 5 hr. period at 37° C. 2. The mean ZO2 values obtained over the first hour for ram, bull and rabbit spermatozoa were 13.6, 13.3 and 7.1 respectively, and the corresponding mean total lactic acid production for each of these species over the 5 hr. period was 297, 439 and 413 µg./108 cells. Significant differences in oxygen uptake, lactic acid production and motility occurred between pooled ejaculates. 3. There was a decline in motility in almost all experiments, and a similar decline in oxygen consumption during the early hours. Towards the end of some experiments on unwashed ram and rabbit spermatozoa there was a rise in oxygen uptake which was shown to be due to bacterial contamination. 4. Washing once had no significant effect on motility, but washing twice adversely affected the motility of ram spermatozoa. 5. Significant decreases in total oxygen uptake occurred on washing ram spermatozoa twice and on washing rabbit spermatozoa both once and twice. This is believed to be due to the removal of bacteria. 6. Washing once had no significant effect on total lactic acid production, but it was significantly reduced on washing ram, bull and rabbit spermatozoa twice. This effect is believed to be associated with the spermatozoa themselves.

scholarly journals CARBOHYDRATE METABOLISM OF SCHISTOSOMA MANSONI

1950 ◽  
Vol 33 (5) ◽  
pp. 475-495 ◽  
Author(s):  
Ernest Bueding
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Schistosoma Mansoni ◽  
Carbohydrate Metabolism ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Dry Weight ◽  
High Rate ◽  
Cyanine Dye ◽  
Low Concentrations

1. Schistosoma mansoni utilizes in 1 hour an amount of glucose equivalent to one-sixth to one-fifth of its dry weight. Over 80 per cent of the metabolized glucose is converted to lactic acid by this organism. 2. The rates of glucose utilization and of lactic acid production by S. mansoni are the same under aerobic and under anaerobic conditions. 3. A high rate of lactic acid production and the absence of a postanaerobic increase in the oxygen uptake differentiate S. mansoni from most other parasitic helminths whose metabolism has been studied. 4. Arsenite and p-chloromercuric benzoate inhibit in low concentrations the oxygen uptake and the rate of glycolysis of S. mansoni. This inhibition is not prevented or reversed by an excess of glutathione or of thioglycollate. 5. Fluoride inhibits the removal of glucose and the production of lactic acid by S. mansoni to the same degree. 6. Low concentrations of quinacrine (atabrine) do not affect the respiration or the carbohydrate metabolism of the schistosomes. 7. The inhibitory effect of aldehydes on the metabolism of S. mansoni has been measured. Among this group of compounds dl-glyceraldehyde and o-nitrobenzaldehyde are the most effective inhibitors of glycolysis. 8. In a concentration of 2.6 x 10–6 M (1:1,000,000) a cyanine dye inhibits almost completely the respiration of the schistosomes, but has no effect on their rate of glycolysis. The oxygen uptake of the worms is inhibited by fuadin to a greater degree than their rate of glycolysis. 2-methyl-1,4-napthoquinone is a much more effective inhibitor of glycolysis than of the respiration of S. mansoni. The latter compound interacts with plasma albumin and, therefore, its inhibitory action on the metabolism of the schistosomes is greatly reduced in human serum or plasma. 9. Evidence is discussed which indicates that, in contrast to glycolysis, respiratory metabolism is not essential for the survival of S. mansoni.

THE METABOLISM OF GLUCOSE BY RAT UTERUS TISSUE IN EARLY PREGNANCY

1971 ◽  
Vol 66 (1) ◽  
pp. 16-24 ◽  
Author(s):  
M. A. H. Surani ◽  
P. J. Heald
Keyword(s):  
Oxygen Consumption ◽  
Lactic Acid ◽  
Oxygen Uptake ◽  
Acid Production ◽  
Energy Requirement ◽  
Lactic Acid Production ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Marked Decline

ABSTRACT The oxygen uptake, lactic acid production and differential production of CO2 from [1-14C] glucose and [6-14C] glucose were examined in rat uterine tissue in vitro during the first nine days of pregnancy. After implantation had occurred the uterine areas containing the implanted blastocysts were studied separately from the remaining areas. It was found that the oxygen consumption of the tissues remained reasonably constant until day 7 of pregnancy and thereafter declined markedly in tissues from implanted areas and non implanted areas when expressed in terms of dry wt. When calculated on a DNA basis, the oxygen consumption of the implanted tissue still showed a marked decline by day 9 of pregnancy, whereas that of the non implanted tissue increased. Lactic acid production increased strongly in the implanted tissue but showed little change in the non implanted areas. The C1/C6 ratio increased significantly on day 3 of pregnancy and was consistantly and significantly higher in the implanted tissue in the non implanted tissue. The results are discussed in terms of the energy requirement for implantation.

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
By Products
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