Atrazine soil extraction techniques for enzyme immunoassay microtiter plate analysis

1992 ◽  
Vol 48 (1) ◽  
Author(s):  
G.K. Stearman ◽  
V.D. Adams
Keyword(s):  
Enzyme Immunoassay ◽  
Microtiter Plate ◽  
Extraction Techniques ◽  
Soil Extraction ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Development of 96 Multiple Injection-GC-MS Technique and Its Application in Protein Engineering of Natural and Non-Natural Enzymatic Reactions

2019 ◽  
Author(s):  
Anja Knorrscheidt ◽  
Pascal Püllmann ◽  
Eugen Schell ◽  
Dominik Homann ◽  
Erik Freier ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Axial Ligand ◽  
Cell System ◽  
Enzymatic Reactions ◽  
The Novel ◽  
Internal Standards ◽  
E Coli ◽  
Enzyme Screening ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Directed evolution requires the screening of enzyme libraries in biological matrices. Available assays are mostly substrate or enzyme specific. Chromatographic techniques like LC and GC overcome this limitation, but require long analysis times. The herein developed multiple injections in a single experimental run (MISER) using GC coupled to MS allows the injection of samples every 33 s resulting in 96-well microtiter plate analysis within 50 min. This technique is implementable in any GC-MS system with autosampling. Since the GC-MS is far less prone to ion suppression than LCMS, no chromatographic separation is required. This allows the utilisation of an internal standards and the detection of main and side-product. To prove the feasibility of the system in enzyme screening, two libraries were assessed: i) YfeX library in an E. coli whole cell system for the carbene-transfer reaction on indole revealing the novel axial ligand tryptophan, ii) a library of 616 chimeras of fungal unspecific peroxygenase (UPO) in S. cerevisiae supernatant for hydroxylation of tetralin resulting in novel constructs. The data quality and representation are automatically assessed by a new R-script.

Bioactivity Profiling Using HPLC/Microtiter-Plate Analysis:  Application to a New Zealand Marine Alga-Derived Fungus,Gliocladiumsp.

2006 ◽  
Vol 69 (4) ◽  
pp. 621-624 ◽  
Author(s):  
Gerhard Lang ◽  
Maya I. Mitova ◽  
Gill Ellis ◽  
Sonia van der Sar ◽  
Richard K. Phipps ◽  
...  
Keyword(s):  
New Zealand ◽  
Marine Alga ◽  
Microtiter Plate ◽  
Analysis Application ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Development of 96 Multiple Injection-GC-MS Technique and Its Application in Protein Engineering of Natural and Non-Natural Enzymatic Reactions

2019 ◽  
Author(s):  
Anja Knorrscheidt ◽  
Pascal Püllmann ◽  
Eugen Schell ◽  
Dominik Homann ◽  
Erik Freier ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Axial Ligand ◽  
Cell System ◽  
Enzymatic Reactions ◽  
The Novel ◽  
Internal Standards ◽  
E Coli ◽  
Enzyme Screening ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Directed evolution requires the screening of enzyme libraries in biological matrices. Available assays are mostly substrate or enzyme specific. Chromatographic techniques like LC and GC overcome this limitation, but require long analysis times. The herein developed multiple injections in a single experimental run (MISER) using GC coupled to MS allows the injection of samples every 33 s resulting in 96-well microtiter plate analysis within 50 min. This technique is implementable in any GC-MS system with autosampling. Since the GC-MS is far less prone to ion suppression than LCMS, no chromatographic separation is required. This allows the utilisation of an internal standards and the detection of main and side-product. To prove the feasibility of the system in enzyme screening, two libraries were assessed: i) YfeX library in an E. coli whole cell system for the carbene-transfer reaction on indole revealing the novel axial ligand tryptophan, ii) a library of 616 chimeras of fungal unspecific peroxygenase (UPO) in S. cerevisiae supernatant for hydroxylation of tetralin resulting in novel constructs. The data quality and representation are automatically assessed by a new R-script.

Enzyme immunosorbent assay of prolactin with penicillinase as label.

1988 ◽  
Vol 34 (11) ◽  
pp. 2205-2207 ◽  
Author(s):  
T G Shrivastav ◽  
P K Pandey ◽  
G L Kumari
Keyword(s):  
Enzyme Activity ◽  
Detection Limit ◽  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Plasma Sample ◽  
Gamma Globulin ◽  
Microtiter Plate ◽  
Plasma Prolactin ◽  
Human Prolactin

Abstract This competitive, rapid, and sensitive enzyme immunosorbent assay for measuring human prolactin in plasma involves penicillinase (EC 3.5.2.6) as label. Microtiter plate wells coated with goat anti-rabbit gamma globulin are filled with antibody to prolactin and plasma sample or reference prolactin and incubated with penicillinase-labeled prolactin for 1 h at 37 degrees C. The enzyme activity of the bound complex is then measured. The assay is as sensitive as radioimmunoassay, the detection limit being 2.5 ng of prolactin per milliliter of plasma. The plasma prolactin values obtained by enzyme immunoassay correlated well with those determined by radioimmunoassay: r = 0.98, slope = 1.00, intercept = 1.11 ng/mL (n = 53).

Indirect Competitive Enzyme Immunoassay for Tetrodotoxin Using a Biotin-Avidin System

1992 ◽  
Vol 75 (5) ◽  
pp. 883-886 ◽  
Author(s):  
Kendo Matsumura ◽  
Sadako Fukiya
Keyword(s):  
Bovine Serum Albumin ◽  
Enzyme Immunoassay ◽  
Immunoglobulin G ◽  
Bovine Serum ◽  
Keyhole Limpet Hemocyanin ◽  
Microtiter Plate ◽  
Mouse Bioassay ◽  
Detection Level ◽  
Standard Curve ◽  
Peroxidase Conjugate

Abstract An indirect competitive enzyme immunoassay was developed to determine tetrodotoxin (TTX). Antiserum against TTX was demonstrated in rabbits immunized with TTX-keyhole limpet hemocyanin conjugate. In this assay, TTX-bovine serum albumin was coated on the microtiter plate, which was incubated with standard TTX and biotinylated immunoglobulin G from antiserum. The amount of antibody bound on the surface of wells was determined by the reaction of avidin-peroxidase conjugate with its substrate. The minimal detection level of TTX ranged from 5 to 25 pg/assay. The standard curve was linear between 0.25 and 50 ng/assay. A good correlation (r= 0.974) for TTX was also obtained between this indirect competitive enzyme immunoassay and the mouse bioassay.

A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

2014 ◽  
Vol 6 (7) ◽  
pp. 2233-2238 ◽  
Author(s):  
Xiaohong Fu ◽  
Xueru Feng ◽  
Kun Xu ◽  
Rong Huang
Keyword(s):  
Enzyme Immunoassay ◽  
Neuron Specific Enolase ◽  
Microtiter Plate ◽  
High Binding ◽  
Monoclonal Mouse

A portable and quantitative enzyme immunoassay with a glucometer readout was developed for the sensitive monitoring of neuron-specific enolase (NSE, as a model analyte) in a high-binding polystyrene 96-well microtiter plate (MTP), conjugated with monoclonal mouse anti-human NSE antibody (mAb1).

Enzyme Immunoassay with Synthetic Peptides to Detect Anti-HTLV-I Antibodies

1992 ◽  
Vol 38 (5) ◽  
pp. 699-703 ◽  
Author(s):  
K Yamada ◽  
N Kuroda ◽  
Y Washitani ◽  
H Shiraki ◽  
Y Maeda
Keyword(s):  
Amino Acids ◽  
Enzyme Immunoassay ◽  
Synthetic Peptides ◽  
Core Protein ◽  
Specific Antigen ◽  
Microtiter Plate ◽  
Type I ◽  
T Lymphotropic Virus ◽  
Amino Terminal ◽  
Lysine Residues

Abstract We developed an enzyme immunoassay (EIA) system to detect antibodies to human T-lymphotropic virus type I (HTLV-I). This system uses chemically synthesized oligopeptides to capture anti-HTLV-I antibodies in serum. The two epitopes of HTLV-I proteins exhibiting the most specific antigen-antibody reaction reside within amino acids 100-130 of p19, a core protein encoded by gag, and amino acids 175-199 of gp46, an envelope glycoprotein encoded by env. This new assay uses synthetic peptides corresponding to these two regions modified by adding two lysine residues at the amino terminal of each peptide to facilitate the binding to the surface of the microtiter plate wells. We compared the performance of our EIA with gelatin-particle-agglutination (PA) and indirect-immunofluorescence (IF) assays, both of which use viral proteins purified from virus-carrying cell cultures. Mass screening by EIA with various synthetic peptides was more accurate than the current confirmatory IF assay.

scholarly journals Practical colorimeter for direct measurement of microplates in enzyme immunoassay systems

1978 ◽  
Vol 7 (1) ◽  
pp. 55-58
Author(s):  
T R Clem ◽  
R H Yolken
Keyword(s):  
Enzyme Immunoassay ◽  
Direct Measurement ◽  
Immunosorbent Assay ◽  
Microtiter Plate ◽  
Enzyme Linked Immunosorbent Assay

A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the specimen to a separate chamber. With this colorimeter, 96 specimens can be read in approximately 5 min. A practical colorimeter such as this can make the use of ELISA tests more feasible for many laboratories.

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