Enzyme immunoassay microtiter plate response to atrazine and metolachlor in potentially interfering matrices

1993 ◽  
Vol 51 (4) ◽  
Author(s):  
G.K. Stearman ◽  
M.J.M. Wells
Keyword(s):  
Enzyme Immunoassay ◽  
Microtiter Plate

Enzyme immunosorbent assay of prolactin with penicillinase as label.

1988 ◽  
Vol 34 (11) ◽  
pp. 2205-2207 ◽  
Author(s):  
T G Shrivastav ◽  
P K Pandey ◽  
G L Kumari
Keyword(s):  
Enzyme Activity ◽  
Detection Limit ◽  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Plasma Sample ◽  
Gamma Globulin ◽  
Microtiter Plate ◽  
Plasma Prolactin ◽  
Human Prolactin

Abstract This competitive, rapid, and sensitive enzyme immunosorbent assay for measuring human prolactin in plasma involves penicillinase (EC 3.5.2.6) as label. Microtiter plate wells coated with goat anti-rabbit gamma globulin are filled with antibody to prolactin and plasma sample or reference prolactin and incubated with penicillinase-labeled prolactin for 1 h at 37 degrees C. The enzyme activity of the bound complex is then measured. The assay is as sensitive as radioimmunoassay, the detection limit being 2.5 ng of prolactin per milliliter of plasma. The plasma prolactin values obtained by enzyme immunoassay correlated well with those determined by radioimmunoassay: r = 0.98, slope = 1.00, intercept = 1.11 ng/mL (n = 53).

Indirect Competitive Enzyme Immunoassay for Tetrodotoxin Using a Biotin-Avidin System

1992 ◽  
Vol 75 (5) ◽  
pp. 883-886 ◽  
Author(s):  
Kendo Matsumura ◽  
Sadako Fukiya
Keyword(s):  
Bovine Serum Albumin ◽  
Enzyme Immunoassay ◽  
Immunoglobulin G ◽  
Bovine Serum ◽  
Keyhole Limpet Hemocyanin ◽  
Microtiter Plate ◽  
Mouse Bioassay ◽  
Detection Level ◽  
Standard Curve ◽  
Peroxidase Conjugate

Abstract An indirect competitive enzyme immunoassay was developed to determine tetrodotoxin (TTX). Antiserum against TTX was demonstrated in rabbits immunized with TTX-keyhole limpet hemocyanin conjugate. In this assay, TTX-bovine serum albumin was coated on the microtiter plate, which was incubated with standard TTX and biotinylated immunoglobulin G from antiserum. The amount of antibody bound on the surface of wells was determined by the reaction of avidin-peroxidase conjugate with its substrate. The minimal detection level of TTX ranged from 5 to 25 pg/assay. The standard curve was linear between 0.25 and 50 ng/assay. A good correlation (r= 0.974) for TTX was also obtained between this indirect competitive enzyme immunoassay and the mouse bioassay.

A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

2014 ◽  
Vol 6 (7) ◽  
pp. 2233-2238 ◽  
Author(s):  
Xiaohong Fu ◽  
Xueru Feng ◽  
Kun Xu ◽  
Rong Huang
Keyword(s):  
Enzyme Immunoassay ◽  
Neuron Specific Enolase ◽  
Microtiter Plate ◽  
High Binding ◽  
Monoclonal Mouse

A portable and quantitative enzyme immunoassay with a glucometer readout was developed for the sensitive monitoring of neuron-specific enolase (NSE, as a model analyte) in a high-binding polystyrene 96-well microtiter plate (MTP), conjugated with monoclonal mouse anti-human NSE antibody (mAb1).

Enzyme Immunoassay with Synthetic Peptides to Detect Anti-HTLV-I Antibodies

1992 ◽  
Vol 38 (5) ◽  
pp. 699-703 ◽  
Author(s):  
K Yamada ◽  
N Kuroda ◽  
Y Washitani ◽  
H Shiraki ◽  
Y Maeda
Keyword(s):  
Amino Acids ◽  
Enzyme Immunoassay ◽  
Synthetic Peptides ◽  
Core Protein ◽  
Specific Antigen ◽  
Microtiter Plate ◽  
Type I ◽  
T Lymphotropic Virus ◽  
Amino Terminal ◽  
Lysine Residues

Abstract We developed an enzyme immunoassay (EIA) system to detect antibodies to human T-lymphotropic virus type I (HTLV-I). This system uses chemically synthesized oligopeptides to capture anti-HTLV-I antibodies in serum. The two epitopes of HTLV-I proteins exhibiting the most specific antigen-antibody reaction reside within amino acids 100-130 of p19, a core protein encoded by gag, and amino acids 175-199 of gp46, an envelope glycoprotein encoded by env. This new assay uses synthetic peptides corresponding to these two regions modified by adding two lysine residues at the amino terminal of each peptide to facilitate the binding to the surface of the microtiter plate wells. We compared the performance of our EIA with gelatin-particle-agglutination (PA) and indirect-immunofluorescence (IF) assays, both of which use viral proteins purified from virus-carrying cell cultures. Mass screening by EIA with various synthetic peptides was more accurate than the current confirmatory IF assay.

scholarly journals Practical colorimeter for direct measurement of microplates in enzyme immunoassay systems

1978 ◽  
Vol 7 (1) ◽  
pp. 55-58
Author(s):  
T R Clem ◽  
R H Yolken
Keyword(s):  
Enzyme Immunoassay ◽  
Direct Measurement ◽  
Immunosorbent Assay ◽  
Microtiter Plate ◽  
Enzyme Linked Immunosorbent Assay

A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the specimen to a separate chamber. With this colorimeter, 96 specimens can be read in approximately 5 min. A practical colorimeter such as this can make the use of ELISA tests more feasible for many laboratories.

Detection of amplifiedChlamydia trachomatis DNA using a microtiter plate-based enzyme immunoassay

1994 ◽  
Vol 13 (9) ◽  
pp. 732-740 ◽  
Author(s):  
J. M. Ossewaarde ◽  
M. Rieffe ◽  
G. J. J. Doornum ◽  
C. J. M. Henquet ◽  
A. M. Loon
Keyword(s):  
Enzyme Immunoassay ◽  
Microtiter Plate

Direct Enzyme Immunoassay of Estradiol in Serum of Women Enrolled in an in Vitro Fertilization and Embryo Transfer Program

1992 ◽  
Vol 38 (8) ◽  
pp. 1409-1413 ◽  
Author(s):  
J Bouve ◽  
J De Boever ◽  
D Leyseele ◽  
E Bosmans ◽  
P Dubois ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Enzyme Immunoassay ◽  
Embryo Transfer ◽  
Microtiter Plate ◽  
Transfer Program ◽  
Vitro Fertilization ◽  
Sexual Steroid ◽  
Embryo Transfer Program ◽  
Assay Time

Abstract We present a fast and simple direct enzyme immunoassay for the sexual steroid hormone estradiol-17 beta in serum of women enrolled in an in vitro fertilization and embryo transfer program. We added 25 microL of standards or samples and 125 microL of a mixture of monoclonal antibody and displacing agents to the wells of a microtiter plate previously coated with a second antibody. After 30 min 50 microL estradiol-horseradish peroxidase conjugate is added. Total incubation time is 75 min. The immunoassay is stopped by washing the microtiter plate. The amount of bound conjugate is then measured at 450 nm with hydrogen peroxide as a substrate and 3,3',5,5'-tetramethylbenzidine as chromogen. The detection limit is 0.24 nmol/L. The assay is thus limited in its application. Analytical recovery is 90.8% (range 80.4-102.9%); within- and between-assay CVs range between 1.3% and 15.4%. Total assay time, including preparation of reagents and data reduction, is only 2.5 h. Results of this enzyme immunoassay compare well with those obtained with four different commercial radioimmunoassays (r = 0.88-0.93).

scholarly journals Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17β in saliva

1997 ◽  
Vol 43 (7) ◽  
pp. 1159-1164 ◽  
Author(s):  
Kenichi Tamate ◽  
Margaret Charleton ◽  
James P Gosling ◽  
Declan Egan ◽  
Mutsuo Ishikawa ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Microtiter Plate ◽  
Cross Reactivity ◽  
Published Data ◽  
Vitro Fertilization ◽  
Menstrual Cycles ◽  
Estradiol 17Β ◽  
Peroxidase Conjugate

Abstract We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17β in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17β–6-carboxymethyloxime–bovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0 − 3 SD) is 365 amol/well or 7.3 pmol/L when 50-μL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is <0.2%. Estradiol-17β was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in ∼3 h and may be useful for fertility monitoring and management.

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