An abscisic acid analog inhibits abscisic acid-induced freezing tolerance and protein accumulation, but not abscisic acid-induced sucrose uptake in a bromegrass (Bromus inermis Leyss) cell culture

Planta ◽  
1996 ◽  
Vol 200 (1) ◽  
Author(s):  
RonaldW. Wilen ◽  
Ping Fu ◽  
AlbertJ. Robertson ◽  
SuzanneR. Abrams ◽  
NicholasH. Low ◽  
...  
Keyword(s):  
Cell Culture ◽  
Abscisic Acid ◽  
Freezing Tolerance ◽  
Bromus Inermis ◽  
Protein Accumulation ◽  
Sucrose Uptake ◽  
Acid Analog

The effects of abscisic acid, kinetin, and gibberellin on freezing tolerance in smooth bromegrass (Bromus inermis) cell suspensions

1989 ◽  
Vol 67 (12) ◽  
pp. 3640-3646 ◽  
Author(s):  
Martin J. T. Reaney ◽  
Lawrence V. Gusta ◽  
Suzanne R. Abrams ◽  
Albert J. Robertson
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Growth Inhibition ◽  
Water Content ◽  
Freezing Tolerance ◽  
Cold Hardiness ◽  
Lethal Effect ◽  
Bromus Inermis ◽  
Cell Water ◽  
Smooth Bromegrass

The effects of kinetin and gibberellic acids (GA3, GA4, GA7, GA9, and a mixture of GA4,7,9) on cold hardening, dehardening, and growth of smooth bromegrass (Bromus inermis Leyss. cv. Manchar) suspension cultures treated with abscisic acid (ABA) were determined. Bromegrass cells treated with 75 μM racemic ABA for 7 days at 25 °C tolerated −37 °C, whereas cells treated with both racemic ABA (75 μM) and a mixture of GA4, GA7, and GA9 (total gibberellic acid concentration 100 μM) were similar in hardiness to the controls (LT50, −10 °C). GA4,7,9 at concentrations greater than 10 μM inhibited the growth of cells. Although 400 μM GA4,7,9 was lethal to cells, 75 μM ABA overcame the lethal effect but did not overcome growth inhibition. The twofold reduction in cell water content due to 75 μM ABA treatment for 7 days was partially overcome by GA4,7,9 at concentrations greater than 400 μM. GA4, GA7, and GA9 were equally effective at limiting growth and inhibiting freezing tolerance induced by ABA, whereas GA3 had little effect on cold hardiness, growth, and water content. During the first 4 days, kinetin at concentrations greater than 100 μM inhibited growth of both control cells and cells treated with ABA. Kinetin (> 100 μM) also inhibited freezing tolerance induced by abscisic acid after 4 days, but had no effect after 8 days. Bromegrass cells treated with 75 μM ABA for 7 days were hardened to −37 °C but dehardened to −12 °C after transfer to fresh medium minus ABA after 12 days at 10 °C. GA4,7,9 (40 μM) had no effect on the rate of dehardening, whereas kinetin increased the rate of dehardening.

scholarly journals Structure-Activity Relationships of Abscisic Acid Analogs Based on the Induction of Freezing Tolerance in Bromegrass (Bromus inermis Leyss) Cell Cultures

1992 ◽  
Vol 100 (4) ◽  
pp. 2024-2029 ◽  
Author(s):  
Grant C. Churchill ◽  
Bruce Ewan ◽  
Martin J. T. Reaney ◽  
Suzanne R. Abrams ◽  
Lawrence V. Gusta
Keyword(s):  
Abscisic Acid ◽  
Cell Cultures ◽  
Freezing Tolerance ◽  
Bromus Inermis ◽  
Structure Activity Relationships ◽  
Structure Activity

scholarly journals Effects of Abscisic Acid Metabolites and Analogs on Freezing Tolerance and Gene Expression in Bromegrass (Bromus inermis Leyss) Cell Cultures

1994 ◽  
Vol 105 (3) ◽  
pp. 823-830 ◽  
Author(s):  
A. J. Robertson ◽  
MJT. Reaney ◽  
R. W. Wilen ◽  
N. Lamb ◽  
S. R. Abrams ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abscisic Acid ◽  
Cell Cultures ◽  
Freezing Tolerance ◽  
Bromus Inermis ◽  
Acid Metabolites

scholarly journals The Halophyte Halostachys caspica AP2/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Fangliu Yin ◽  
Youling Zeng ◽  
Jieyun Ji ◽  
Pengju Wang ◽  
Yufang Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Freezing Tolerance ◽  
Critical Role ◽  
Extreme Temperature ◽  
Freezing Stress ◽  
Antioxidant Enzyme Activities ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Halostachys Caspica

The APETALA2 (AP2) and ethylene-responsive element-binding factor (ERF) gene family is one of the largest plant-specific transcription factor gene families, which plays a critical role in plant development and evolution, as well as response to various stresses. The TARGET OF EAT3 (TOE3) gene is derived from Halostachys caspica and belongs to the AP2 subfamily with two AP2 DNA-binding domains. Currently, AP2 family mainly plays crucial roles in plant growth and evolution, yet there are few reports about the role of AP2 in abiotic stress tolerance. Here, we report HcTOE3, a new cold-regulated transcription factor gene, which has an important contribution to freezing tolerance. The main results showed that the expression of HcTOE3 in the H. caspica assimilating branches was strongly induced by different abiotic stresses, including high salinity, drought, and extreme temperature (heat, chilling, and freezing), as well as abscisic acid and methyl viologen treatments. Overexpressing HcTOE3 gene (OE) induced transgenic Arabidopsis plant tolerance to freezing stress. Under freezing treatment, the OE lines showed lower content of malondialdehyde and electrolyte leakage and less accumulation of reactive oxygen species compared with the wild type. However, the survival rates, antioxidant enzyme activities, and contents of osmotic adjustment substance proline were enhanced in transgenic plants. Additionally, the OE lines increased freezing tolerance by up-regulating the transcription level of cold responsive genes (CBF1, CBF2, COR15, COR47, KIN1, and RD29A) and abscisic acid signal transduction pathway genes (ABI1, ABI2, ABI5, and RAB18). Our results suggested that HcTOE3 positively regulated freezing stress and has a great potential as a candidate gene to improve plant freezing tolerance.

scholarly journals Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

2020 ◽  
Vol 21 (16) ◽  
pp. 5899 ◽  
Author(s):  
Adam Augustyniak ◽  
Izabela Pawłowicz ◽  
Katarzyna Lechowicz ◽  
Karolina Izbiańska-Jankowska ◽  
Magdalena Arasimowicz-Jelonek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Cold Acclimation ◽  
Antioxidant System ◽  
Freezing Tolerance ◽  
Photosynthetic Apparatus ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Protein Accumulation ◽  
Oxygen Species

Though winter-hardiness is a complex trait, freezing tolerance was proved to be its main component. Species from temperate regions acquire tolerance to freezing in a process of cold acclimation, which is associated with the exposure of plants to low but non-freezing temperatures. However, mechanisms of cold acclimation in Lolium-Festuca grasses, important for forage production in Europe, have not been fully recognized. Thus, two L. multiflorum/F. arundinacea introgression forms with distinct freezing tolerance were used herein as models in the comprehensive research to dissect these mechanisms in that group of plants. The work was focused on: (i) analysis of cellular membranes’ integrity; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gas exchange; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); (iii) analysis of plant antioxidant capacity (reactive oxygen species generation; gene expression, protein accumulation, and activity of selected enzymes); and (iv) analysis of Cor14b accumulation, under cold acclimation. The more freezing tolerant introgression form revealed a higher integrity of membranes, an ability to cold acclimate its photosynthetic apparatus and higher water use efficiency after three weeks of cold acclimation, as well as a higher capacity of the antioxidant system and a lower content of reactive oxygen species in low temperature.

scholarly journals Water Content during Abscisic Acid Induced Freezing Tolerance in Bromegrass Cells

1990 ◽  
Vol 93 (2) ◽  
pp. 460-464 ◽  
Author(s):  
Karen Tanino ◽  
Conrad J. Weiser ◽  
Leslie H. Fuchigami ◽  
Tony H. H. Chen
Keyword(s):  
Abscisic Acid ◽  
Water Content ◽  
Freezing Tolerance
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