Determination of diphtheria toxin neutralizing antibody titers with a cell protein synthesis inhibition assay

1991 ◽  
Vol 180 (1) ◽  
Author(s):  
Elisabetta Padovan ◽  
Emanuele Papini ◽  
Rino Rappuoli ◽  
Cesare Montecucco
Keyword(s):  
Protein Synthesis ◽  
Diphtheria Toxin ◽  
Neutralizing Antibody ◽  
Cell Protein ◽  
Protein Synthesis Inhibition ◽  
Inhibition Assay ◽  
Synthesis Inhibition ◽  
Antibody Titers ◽  
A Cell

Cell-free protein synthesis inhibition assay for the cyanobacterial toxin cylindrospermopsin

2001 ◽  
Vol 16 (5) ◽  
pp. 408-412 ◽  
Author(s):  
Suzanne M. Froscio ◽  
Andrew R. Humpage ◽  
Philip C. Burcham ◽  
Ian R. Falconer
Keyword(s):  
Protein Synthesis ◽  
Protein Synthesis Inhibition ◽  
Inhibition Assay ◽  
Synthesis Inhibition ◽  
Free Protein ◽  
Cyanobacterial Toxin ◽  
Cell Free Protein Synthesis

Presence of antibody in virus-infected brain cells of SSPE ferrets

1983 ◽  
Vol 41 ◽  
pp. 804-805
Author(s):  
Hannah R. Brown ◽  
Tammy L. Donato ◽  
Halldor Thormar
Keyword(s):  
Measles Virus ◽  
Plasma Cells ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Neutralizing Antibody ◽  
Brain Cells ◽  
Antibody Titers ◽  
A Cell ◽  
The Central Nervous System ◽  
The Brain

Measles virus specific immunoglobulin G (IgG) has been found in the brains of patients with subacute sclerosing panencephalitis (SSPE), a slowly progressing disease of the central nervous system (CNS) in children. IgG/albumin ratios indicate that the antibodies are synthesized within the CNS. Using the ferret as an animal model to study the disease, we have been attempting to localize the Ig's in the brains of animals inoculated with a cell associated strain of SSPE. In an earlier report, preliminary results using Protein A conjugated to horseradish peroxidase (PrAPx) (Dynatech Diagnostics Inc., South Windham, ME.) to detect antibodies revealed the presence of immunoglobulin mainly in antibody-producing plasma cells in inflammatory lesions and not in infected brain cells.In the present experiment we studied the brain of an SSPE ferret with neutralizing antibody titers of 1:1024 in serum and 1:512 in CSF at time of sacrifice 7 months after i.c. inoculation with SSPE measles virus-infected cells. The animal was perfused with saline and portions of the brain and spinal cord were immersed in periodate-lysine-paraformaldehyde (P-L-P) fixative. The ferret was not perfused with fixative because parts of the brain were used for virus isolation.

Protein synthesis inhibition alters Drosophila mating behavior

1977 ◽  
Vol 6 (3) ◽  
pp. 355-357 ◽  
Author(s):  
Anita Pruzan ◽  
Philip B. Applewhite ◽  
Michael J. Bucci
Keyword(s):  
Protein Synthesis ◽  
Mating Behavior ◽  
Protein Synthesis Inhibition ◽  
Synthesis Inhibition

Focal protein synthesis inhibition in a model of neonatal hypoxic-ischemic brain injury

1987 ◽  
Vol 95 (2) ◽  
pp. 277-289 ◽  
Author(s):  
Barney E. Dwyer ◽  
Robert N. Nishimura ◽  
Clydette L. Powell ◽  
Susan L. Mailheau
Keyword(s):  
Protein Synthesis ◽  
Brain Injury ◽  
Ischemic Brain Injury ◽  
Protein Synthesis Inhibition ◽  
Ischemic Brain ◽  
Synthesis Inhibition ◽  
Hypoxic Ischemic Brain Injury
Sign in / Sign up

Export Citation Format

Share Document