Simulation of cell growth, substrate consumption and product formation with recombinant Escherichia coli

1991 ◽  
Vol 35 (2) ◽  
Author(s):  
G. Korte ◽  
U. Rinas ◽  
H.-A. Kracke-Helm ◽  
K. Sch�gerl
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Product Formation ◽  
Recombinant Escherichia Coli ◽  
Growth Substrate ◽  
Substrate Consumption

scholarly journals Application Of The Z And Laplace Transforms To Panification Yeast Production Using An Airlift Bioreactor

2020 ◽  
Author(s):  
Arnaldo Silva Oliveira ◽  
Juan C. B. Neto ◽  
Igor J. B. Santos ◽  
Edson R. Nucci
Keyword(s):  
Cell Growth ◽  
Product Formation ◽  
Laplace Transforms ◽  
Discrete Models ◽  
Average Error ◽  
Growth Substrate ◽  
Substrate Consumption ◽  
Yeast Saccharomyces Cerevisiae ◽  
Mathematical Techniques ◽  
Discrete Time Models

Abstract The Z- and Laplace transforms are mathematical techniques applied to solve difference equations and differential equations, respectively. Mathematical models used to describe cell growth, substrate consumption and product formation in bioprocesses can be represented by these types of equations. Thus, in this work, the fermentation process of the yeast Saccharomyces cerevisiae was modeled using different models from the literature, and the Z- and Laplace transforms were applied to solve the equations. Once the equations were solved, the models were represented in state space and simulated in Octave® software. Finally, the models were compared to experimental data from previous studies and to each other. Verhulst was the model that best described the process, with an average error of 4.74% for cell growth and 13.9% for substrate consumption. This work is unprecedented since no works that use the Z transform and discrete models for the representation of fermentation of this yeast were found in the literature. Even more importantly, this work proved that discrete-time models can be applied to bioprocesses with the same precision as continuous-time models.

The Study of the Kinetic Model of Halomonas Salina Fermenting Ectoine

2013 ◽  
Vol 781-784 ◽  
pp. 647-652
Author(s):  
Shuang Gao ◽  
Ling Hua Zhang ◽  
Qing Chen ◽  
Lin Bai ◽  
Ya Jun Lang
Keyword(s):  
Cell Growth ◽  
Kinetic Model ◽  
Monosodium Glutamate ◽  
Biomass Concentration ◽  
Correlation Coefficients ◽  
Kinetic Mechanism ◽  
Product Formation ◽  
Substrate Consumption ◽  
Nonlinear Fitting ◽  
Potential Applications

Ectoine had important physiological functions and superior potential applications, so the study of ectoine was extensively attented. This article was related to kinetic models of cell growth, product formation and substrate consumption, which was not only established according to the characteristics of ectoine batch fermentation by Halomonas salina DSM 5928 but also obtained the kinetic parameters by the nonlinear fitting method in the Microcal Origin software. Logistic, Luedeking-Piret and Luedeking-Piret-like equations was applied to analyze the cell growth, the ectoine formation and the substrate consumption by the kinetic model，respectively. The results between calculated values and experimental data were coincident. By fitting, correlation coefficients R2 were ≥ 0.989. The fermentation conditions of ectoine were analyzed according to the model. The results showed that ectoine productivity (0.28 g/L/h) was the highest when initial monosodium glutamate concentration (S0) was 60 g/L. However, when S0 was 80 g/L, the ectoine concentration was maximal, i.e., 7.59 g/L. The research suggested that ectoine formation belonged to the mixed kinetic mechanism of cell growth and biomass concentration, while the ectoine production mainly depended on instantaneous biomass concentration. The fermentation method for improving ectoine concentration was further proved. The established kinetic model will be of significant value to provide the optimal conditions of present process.

Optimization of culture medium for cell growth and expression of 648 antigen from Leishmania infantum chagasi in recombinant Escherichia coli M15

2014 ◽  
Vol 65 (3) ◽  
pp. 1607-1613 ◽  
Author(s):  
Michelle Rossana Ferreira Vaz ◽  
Francisco Canindé de Sousa Junior ◽  
Letícia Maia Resende Costa ◽  
Everaldo Silvino dos Santos ◽  
Daniella Regina Arantes Martins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Leishmania Infantum ◽  
Culture Medium ◽  
Recombinant Escherichia Coli ◽  
Leishmania Infantum Chagasi

scholarly journals Kinetic Studies on Fermentative Production of Biofuel from Synthesis Gas UsingClostridium ljungdahlii

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Maedeh Mohammadi ◽  
Abdul Rahman Mohamed ◽  
Ghasem D. Najafpour ◽  
Habibollah Younesi ◽  
Mohamad Hekarl Uzir
Keyword(s):  
Cell Growth ◽  
Synthesis Gas ◽  
Uptake Rate ◽  
Kinetic Studies ◽  
Product Formation ◽  
Volterra Model ◽  
Substrate Uptake ◽  
Growth Substrate ◽  
Clostridium Ljungdahlii ◽  
Batch Bioreactors

The intrinsic growth, substrate uptake, and product formation biokinetic parameters were obtained for the anaerobic bacterium,Clostridium ljungdahlii, grown on synthesis gas in various pressurized batch bioreactors. A dual-substrate growth kinetic model using Luong for CO and Monod for H2was used to describe the growth kinetics of the bacterium on these substrates. The maximum specific growth rate (μmax= 0.195 h−1) and Monod constants for CO (Ks,CO= 0.855 atm) and H2(Ks,H2= 0.412 atm) were obtained. This model also accommodated the CO inhibitory effects on cell growth at high CO partial pressures, where no growth was apparent at high dissolved CO tensions (PCO∗>0.743 atm). The Volterra model, Andrews, and modified Gompertz were, respectively, adopted to describe the cell growth, substrate uptake rate, and product formation. The maximum specific CO uptake rate (qmax= 34.364 mmol/gcell/h), CO inhibition constant (KI= 0.601 atm), and maximum rate of ethanol (Rmax= 0.172 mmol/L/h atPCO= 0.598 atm) and acetate (Rmax= 0.096 mmol/L/h atPCO= 0.539 atm) production were determined from the applied models.

scholarly journals Poly(4-hydroxybutyrate) (P4HB) production in recombinant Escherichia coli: P4HB synthesis is uncoupled with cell growth

2013 ◽  
Vol 12 (1) ◽  
pp. 123 ◽  
Author(s):  
Sylvaine Le Meur ◽  
Manfred Zinn ◽  
Thomas Egli ◽  
Linda Thöny-Meyer ◽  
Qun Ren
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Recombinant Escherichia Coli

scholarly journals Construction of Efficient Platform Escherichia coli Strains for Polyhydroxyalkanoate Production by Engineering Branched Pathway

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 509 ◽  
Author(s):  
Hye-Rim Jung ◽  
Su-Yeon Yang ◽  
Yu-Mi Moon ◽  
Tae-Rim Choi ◽  
Hun-Suk Song ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Carbon Flux ◽  
Recombinant Escherichia Coli ◽  
Acetyl Coa ◽  
E Coli ◽  
Efficient Conversion ◽  
Pha Production

Polyhydroxyalkanoate (PHA) is a potential substitute for petroleum-based plastics and can be produced by many microorganisms, including recombinant Escherichia coli. For efficient conversion of substrates and maximum PHA production, we performed multiple engineering of branched pathways in E. coli. We deleted four genes (pflb, ldhA, adhE, and fnr), which contributed to the formation of byproducts, using the CRISPR/Cas9 system and overexpressed pntAB, which catalyzes the interconversion of NADH and NADPH. The constructed strain, HR002, showed accumulation of acetyl-CoA and decreased levels of byproducts, resulting in dramatic increases in cell growth and PHA content. Thus, we demonstrated the effects of multiple engineering for redirecting carbon flux into PHA production without any concerns regarding simultaneous deletion.

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