Electron and fluorescence microscopic investigations on composition and structure of the epithelial basement membrane of the human inferior nasal concha

1993 ◽  
Vol 250 (7) ◽  
Author(s):  
P. Agha-Mir-Salim ◽  
O. Rauhut ◽  
H.-J. Merker
Keyword(s):  
Basement Membrane ◽  
Epithelial Basement Membrane ◽  
Composition And Structure ◽  
Nasal Concha ◽  
Inferior Nasal Concha ◽  
Epithelial Basement ◽  
Microscopic Investigations

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Increased glycosylation of human lens epithelial basement membrane in diabetes mellitus

1994 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Dong H. Shin ◽  
Shlomo S. Mandel ◽  
Jin H. Lee ◽  
Brett Ernst ◽  
Bruce L. Newman ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Basement Membrane ◽  
Human Lens ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement

scholarly journals Disruption of respiratory epithelial basement membrane in COVID-19 patients

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Xue Liu ◽  
Yinshan Fang ◽  
Paul W. Noble ◽  
Jianwen Que ◽  
Dianhua Jiang
Keyword(s):  
Basement Membrane ◽  
Epithelial Basement Membrane ◽  
Respiratory Epithelial ◽  
Epithelial Basement

A Monoclonal Antibody which Identifies an Antigen in Endothelial Cell and Epithelial Basement Membrane

1990 ◽  
Vol 27 (1) ◽  
pp. 14-23
Author(s):  
Shinobu Matsuo ◽  
Neal S. Penneys ◽  
Jo-David Fine ◽  
Steffen Gay ◽  
Mehrdad Nadji
Keyword(s):  
Monoclonal Antibody ◽  
Endothelial Cell ◽  
Basement Membrane ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement

scholarly journals Alveolar epithelial surface area-volume relationship in isolated rat lungs

1999 ◽  
Vol 86 (6) ◽  
pp. 2026-2033 ◽  
Author(s):  
Daniel J. Tschumperlin ◽  
Susan S. Margulies
Keyword(s):  
Basement Membrane ◽  
Surface Area ◽  
Total Lung Capacity ◽  
Membrane Surface ◽  
Epithelial Surface ◽  
Alveolar Epithelial ◽  
Lung Capacity ◽  
Epithelial Basement Membrane ◽  
Rat Lungs ◽  
Epithelial Basement

In vitro studies of the alveolar epithelial response to deformation require knowledge of the in situ mechanical environment of these cells. Because of the presence of tissue folding and crumpling, previous measurements of the alveolar surface area available for gas exchange are not equivalent to the epithelial surface area. To identify epithelial deformations in uniformly inflated lungs representative of the in vivo condition, we studied isolated Sprague-Dawley rat lungs ( n = 31) fixed by perfusion with glutaraldehyde on deflation after cycling three times at high lung volume (10–25 cmH2O). The epithelial basement membrane in 45 electron micrographs (×12,000)/rat was traced, digitally scanned, and analyzed. Epithelial basement membrane surface area (EBMSA) was computed from a morphometric relationship. EBMSA was found to increase 5, 16, 12, and 40% relative to EBMSA at 24% total lung capacity at lung volumes of 42, 60, 82, and 100% total lung capacity, respectively. The increases in EBMSA suggest that epithelial cells undergo significant deformations with large inflations and that alveolar basement membrane deformation may contribute to lung recoil at high lung pressures.

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