Production of Phanerochaete chrysosporium lignin peroxidase under various culture conditions

1993 ◽  
Vol 40 (4) ◽  
Author(s):  
Reetta Haapala ◽  
Susan Linko
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Culture Conditions

Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus

2009 ◽  
Vol 55 (12) ◽  
pp. 1397-1402 ◽  
Author(s):  
Paramjit Kaur Bajwa ◽  
Daljit Singh Arora
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Ligninolytic Enzymes ◽  
Culture Conditions ◽  
Malt Extract ◽  
Mineral Salts ◽  
Wide Range ◽  
Extract Medium ◽  
Malt Extract Medium

The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium . Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.

scholarly journals A Homokaryotic Derivative of a Phanerochaete chrysosporium Strain and Its Use in Genomic Analysis of Repetitive Elements

2000 ◽  
Vol 66 (4) ◽  
pp. 1629-1633 ◽  
Author(s):  
Philip Stewart ◽  
Jill Gaskell ◽  
Daniel Cullen
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Pcr Amplification ◽  
Genomic Analysis ◽  
Gene Families ◽  
Culture Conditions ◽  
Repetitive Elements ◽  
Reverse Transcription Pcr ◽  
Multiple Copies ◽  
Nuclear Condition

ABSTRACT Analysis of complex gene families in the lignin-degrading basidiomycete Phanerochaete chrysosporium has been hampered by the dikaryotic nuclear condition. To facilitate genetic investigations in P. chrysosporium strain BKM-F-1767, we isolated a homokaryon from regenerated protoplasts. The nuclear condition was established by PCR amplification of five unlinked genes followed by probing with allele-specific oligonucleotides. Under standard nitrogen-limited culture conditions, lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities of the homokaryon were equivalent to those of the parental dikaryon. We used the homokaryon to determine the genomic organization and to assess transcriptional effects of a family of repetitive elements. Previous studies had identified an insertional mutation, Pce1, within lignin peroxidase allele lipI2. The element resembled nonautonomous class II transposons and was present in multiple copies in strain BKM-F-1767. In the present study, three additional copies of the Pce1-like element were cloned and sequenced. The distribution of elements was nonrandom; all localized to the same 3.7-Mb chromosome, as assessed by segregation analysis and Southern blot analysis of the homokaryon. Reverse transcription-PCR (RT-PCR) showed that Pce1 was not spliced from thelipI2 transcript in either the homokaryon or the parental dikaryon. However, both strains had equivalent lignin peroxidase activity, suggesting that some lip genes may be redundant.

Molecular cloning and sequences of lignin peroxidase genes of Phanerochaete chrysosporium

1989 ◽  
Vol 9 (6) ◽  
pp. 2743-2747
Author(s):  
H Schalch ◽  
J Gaskell ◽  
T L Smith ◽  
D Cullen
Keyword(s):  
Molecular Cloning ◽  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Nucleotide Sequences ◽  
Peroxidase Isozyme ◽  
Genomic Clones

The genomic clones encoding lignin peroxidase isozyme H8 and two closely related genes were isolated from Phanerochaete chrysosporium BKM-1767, and their nucleotide sequences were determined. The positions and approximate lengths of introns were found to be highly conserved in all three clones. Analysis of homokaryotic derivatives indicated that the three clones are not alleles of the same gene(s).

scholarly journals Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium.

1990 ◽  
Vol 172 (1) ◽  
pp. 260-265 ◽  
Author(s):  
K Boominathan ◽  
S B Dass ◽  
T A Randall ◽  
R L Kelley ◽  
C A Reddy
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
White Rot ◽  
Negative Mutant
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