Alternative respiration and antibiotic production of Acremonium chrysogenum

1993 ◽  
Vol 40 (4) ◽  
Author(s):  
J�zsef Kozma ◽  
Luz Lucas ◽  
Karl Sch�gerl
Keyword(s):  
Antibiotic Production ◽  
Acremonium Chrysogenum ◽  
Alternative Respiration

Stimulation of the cyanide-resistant alternative respiratory pathway by oxygen in Acremonium chrysogenum correlates with the size of the intracellular peroxide pool

2003 ◽  
Vol 49 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Levente Karaffa ◽  
Erzsébet Sándor ◽  
Erzsébet Fekete ◽  
József Kozma ◽  
Attila Szentirmai ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Respiratory Activity ◽  
Peroxyl Radical ◽  
Radical Scavenger ◽  
Acremonium Chrysogenum ◽  
Alternative Respiration ◽  
Respiratory Pathway ◽  
Submerged Cultures ◽  
Alternative Respiratory Pathway ◽  
The Relationship

The relationship between oxygen input and activity of the cyanide-resistant alternative respiration of submerged cultures of Acremonium crysogenum was investigated. The volumetric oxygen transfer coefficient of the respective cultures correlated positively within almost two ranges of magnitude with the size of the intracellular peroxide pool, which in turn, correlated with the activity of the cyanide-resistant alternative respiratory pathway. Increased aeration also stimulated the glucose uptake rate but had no effect on the total respiration rate or the growth rate. Addition of the lipid peroxyl radical scavenger DL-α-tocopherol to A. chrysogenum cultures decreased the rate of intracellular peroxide production as well as glucose uptake. An increase in the cyanide-resistant fraction of total respiration was observed, while growth and the total respiratory activity remained unchanged. We conclude that intracellular peroxides may stimulate the alternative respiration in A. chrysogenum.Key words: Acremonium chrysogenum, alternative respiration, oxygen, peroxide, Kla.

Alternative respiration of Acremonium chrysogenum

1991 ◽  
Vol 13 (12) ◽  
pp. 899-900 ◽  
Author(s):  
J�zsef Kozma ◽  
Luz Lucas ◽  
Karl Sch�gerl
Keyword(s):  
Acremonium Chrysogenum ◽  
Alternative Respiration

Cephalosporin-C production, morphology and alternative respiration of Acremonium chrysogenum in glucose-limited chemostat

1996 ◽  
Vol 18 (6) ◽  
pp. 701-706 ◽  
Author(s):  
Levente Karaffa ◽  
Erzs�bet S�ndor ◽  
J�zsef Kozma ◽  
Attila Szentirmai
Keyword(s):  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Alternative Respiration ◽  
Cephalosporin C Production

scholarly journals The Fennoscandian Shield deep terrestrial virosphere suggests slow motion ‘boom and burst’ cycles

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Karin Holmfeldt ◽  
Emelie Nilsson ◽  
Domenico Simone ◽  
Margarita Lopez-Fernandez ◽  
Xiaofen Wu ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Nutrient Recycling ◽  
Slow Motion ◽  
Deep Biosphere ◽  
Wide Range ◽  
Domains Of Life ◽  
Microbial Turnover ◽  
Rna Transcript ◽  
Viral Isolates ◽  
Antagonistic Interactions

AbstractThe deep biosphere contains members from all three domains of life along with viruses. Here we investigate the deep terrestrial virosphere by sequencing community nucleic acids from three groundwaters of contrasting chemistries, origins, and ages. These viromes constitute a highly unique community compared to other environmental viromes and sequenced viral isolates. Viral host prediction suggests that many of the viruses are associated with Firmicutes and Patescibacteria, a superphylum lacking previously described active viruses. RNA transcript-based activity implies viral predation in the shallower marine water-fed groundwater, while the deeper and more oligotrophic waters appear to be in ‘metabolic standby’. Viral encoded antibiotic production and resistance systems suggest competition and antagonistic interactions. The data demonstrate a viral community with a wide range of predicted hosts that mediates nutrient recycling to support a higher microbial turnover than previously anticipated. This suggests the presence of ‘kill-the-winner’ oscillations creating slow motion ‘boom and burst’ cycles.

scholarly journals Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 424
Author(s):  
Osama G. Mohamed ◽  
Sadaf Dorandish ◽  
Rebecca Lindow ◽  
Megan Steltz ◽  
Ifrah Shoukat ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Gene Clusters ◽  
Multidrug Resistant ◽  
Microbial Interactions ◽  
Mass Spectrometry Data ◽  
Metagenomic Data ◽  
Resistant Bacteria ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Plate Technique

The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth®, we isolated over 1400 antibiotic-producing microbes, including 62, showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant Staphylococcus aureus. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production.

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