Cephalosporin-C production, morphology and alternative respiration of Acremonium chrysogenum in glucose-limited chemostat

1996 ◽  
Vol 18 (6) ◽  
pp. 701-706 ◽  
Author(s):  
Levente Karaffa ◽  
Erzs�bet S�ndor ◽  
J�zsef Kozma ◽  
Attila Szentirmai
Keyword(s):  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Alternative Respiration ◽  
Cephalosporin C Production

A Myb transcription factor represses conidiation and cephalosporin C production in Acremonium chrysogenum

2018 ◽  
Vol 118 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Wang ◽  
Pengjie Hu ◽  
Honghua Li ◽  
Yanling Wang ◽  
Liang-kun Long ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Cephalosporin C Production

Utilization of algal sugars and glycerol for enhanced cephalosporin C production by Acremonium chrysogenum M35

2016 ◽  
Vol 64 (1) ◽  
pp. 66-72 ◽  
Author(s):  
J.H. Lee ◽  
H.Y. Yoo ◽  
X. Yang ◽  
D.S. Kim ◽  
J.H. Lee ◽  
...  
Keyword(s):  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Cephalosporin C Production

Fatty acids reduce the tensile strength of fungal hyphae during cephalosporin C production in Acremonium chrysogenum

2006 ◽  
Vol 29 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Jong Chae Kim ◽  
Yoon Seok Song ◽  
Dong Hwan Lee ◽  
Seong Woo Kang ◽  
Seung Wook Kim
Keyword(s):  
Fatty Acids ◽  
Tensile Strength ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Fungal Hyphae ◽  
Cephalosporin C Production

Analysis of the relationship between growth, cephalosporin C production, and fragmentation in Acremonium chrysogenum

2001 ◽  
Vol 47 (9) ◽  
pp. 801-806 ◽  
Author(s):  
Erzsébet Sándor ◽  
Attila Szentirmai ◽  
Gopal C. Paul ◽  
Colin R. Thomas ◽  
István Pócsi ◽  
...  
Keyword(s):  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Cephalosporin C Production ◽  
The Relationship

Analysis of the relationship between growth, cephalosporin C production, and fragmentation in Acremonium chrysogenum

2001 ◽  
Vol 47 (9) ◽  
pp. 801-806 ◽  
Author(s):  
Erzsébet Sándor ◽  
Attila Szentirmai ◽  
Gopal C Paul ◽  
Colin R Thomas ◽  
István Pócsi ◽  
...  
Keyword(s):  
Image Analysis ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Fed Batch ◽  
Submerged Cultures ◽  
Batch Cultures ◽  
Chemostat Cultures ◽  
Fed Batch Fermentation ◽  
Cephalosporin C Production ◽  
The Relationship

Mycelial fragmentation in submerged cultures of the cephalosporin C (CPC) producing fungus Acremonium chrysogenum was characterized by image analysis. In both fed-batch and chemostat cultures, the proportion of mycelial clumps seemed to be the most sensitive morphological indicator of fragmentation. In a fed-batch fermentation culture, this declined from roughly 60% at inoculation to less than 10% after 43 h. Subsequent additions of glucose resulted in a sharp increase back to near the initial value, an increase that reversed itself a few hours after glucose exhaustion. Meanwhile CPC production continued to decline steadily. On the other hand, the addition of soybean oil enhanced CPC production, but had no significant effect on the morphology. Although it may sometimes appear that morphology and productivity are related in batch or fed-batch cultures, this study suggests that this is because both respond simultaneously to more fundamental physiological changes, dependent on the availability of carbon. In circumstances, such as supplementary carbon source addition, the relationship is lost. Chemostat cultures supported this belief, as CPC-production rates were hardly affected by the specific growth rate, but the morphology showed significant differences, i.e., lower dilution rates resulted in a lower proportion of clumps and in smaller clumps.Key words: image analysis, Acremonium chrysogenum, morphology, fragmentation, cephalosporin C.

scholarly journals Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding Acremonium chrysogenum Strain

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6636
Author(s):  
Alexander A. Zhgun ◽  
Mikhail A. Eldarov
Keyword(s):  
Secondary Metabolites ◽  
Filamentous Fungi ◽  
Morphological Changes ◽  
Random Mutagenesis ◽  
Penicillin G ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Beta Lactam ◽  
Highly Active ◽  
Cephalosporin C Production

The high-yielding production of pharmaceutically significant secondary metabolites in filamentous fungi is obtained by random mutagenesis; such changes may be associated with shifts in the metabolism of polyamines. We have previously shown that, in the Acremonium chrysogenum cephalosporin C high-yielding strain (HY), the content of endogenous polyamines increased by four- to five-fold. Other studies have shown that the addition of exogenous polyamines can increase the production of target secondary metabolites in highly active fungal producers, in particular, increase the biosynthesis of β-lactams in the Penicillium chrysogenum Wis 54–1255 strain, an improved producer of penicillin G. In the current study, we demonstrate that the introduction of exogenous polyamines, such as spermidine or 1,3-diaminopropane, to A. chrysogenum wild-type (WT) and HY strains, leads to an increase in colony germination and morphological changes in a complete agar medium. The addition of 5 mM polyamines during fermentation increases the production of cephalosporin C in the A. chrysogenum HY strain by 15–20% and upregulates genes belonging to the beta-lactam biosynthetic cluster. The data obtained indicate the intersection of the metabolisms of polyamines and beta-lactams in A. chrysogenum and are important for the construction of improved producers of secondary metabolites in filamentous fungi.

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