N-CAM, polysialic acid and chick tail bud development

1991 ◽  
Vol 183 (2) ◽  
Author(s):  
C.May Griffith ◽  
MichaelJ. Wiley
Keyword(s):  
Polysialic Acid ◽  
Tail Bud ◽  
Bud Development

Sialoconjugates and development of the tail bud

Development ◽  
1990 ◽  
Vol 108 (3) ◽  
pp. 479-489
Author(s):  
C.M. Griffith ◽  
M.J. Wiley
Keyword(s):  
Sialic Acid ◽  
Lectin Histochemistry ◽  
Limulus Polyphemus ◽  
Chick Embryos ◽  
Developmental Sequence ◽  
Tail Bud ◽  
Bud Development ◽  
Stage Of Development ◽  
Higher Doses ◽  
Axial Development

Using lectin histochemistry, we have previously shown that there are alterations in the distribution of glycoconjugates in the tail bud of chick embryos that parallel the developmental sequence of the caudal axis. If glycoconjugates or the cells bearing them play a role in caudal axial development, then, restriction of their availability by binding with lectins would be expected to produce abnormalities of caudal development. In the present study, we treated embryos at various stages of tail bud development by microinjection with a variety of lectins. Administration of WGA by sub-blastodermal injection resulted in high incidences of secondary neural tube and notochordal abnormalities in lectin-treated embryos. The incidence of malformations was dependent upon both the dose of WGA received and the stage of development at the time of treatment. Using an anti-WGA antibody, we have also shown binding of the lectin in regions where defects were found. The lectin WGA binds to the sialic acid residues of glycoconjugates and to N-acetylglucosamine. Treatment of embryos with Limulus polyphemus lectin (LPL), which also binds to sialic acid, produced results similar to those of WGA. Treatments using lectins with other sugar-binding specificities, including succinylated WGA (with N-acetylglucosamine specificity only) produced defects that differed from those produced by WGA and LPL, and only with the administration of much higher doses. The results suggest that glycoconjugates in general and sialoconjugates in particular, or the cells carrying them, may have a role in caudal axial development.

Effects of retinoic acid on chick tail bud development

Teratology ◽  
1991 ◽  
Vol 43 (3) ◽  
pp. 217-224 ◽  
Author(s):  
C. May Griffith ◽  
Michael J. Wiley
Keyword(s):  
Retinoic Acid ◽  
Tail Bud ◽  
Bud Development

Effects of retinoic acid on the distribution of glycoconjugates during mouse tail bud development

Teratology ◽  
1990 ◽  
Vol 41 (3) ◽  
pp. 281-288 ◽  
Author(s):  
C. May Griffith ◽  
Michael J. Wiley
Keyword(s):  
Retinoic Acid ◽  
Tail Bud ◽  
Bud Development

Morphological Features of Tail Bud Development in Truncate Mouse Mutants

2004 ◽  
Vol 178 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Dinko Mitrečić ◽  
Ljiljana Kostović-Knežević ◽  
Srećko Gajović
Keyword(s):  
Morphological Features ◽  
Tail Bud ◽  
Bud Development ◽  
Mouse Mutants

A Leaf Bud Development Scale for Rabbiteye Blueberry

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 455f-456 ◽  
Author(s):  
D. Scott NeSmith ◽  
Gerard Krewer ◽  
Jeffrey G. Williamson
Keyword(s):  
Reproductive Development ◽  
Developmental Differences ◽  
Research Management ◽  
Growth Stages ◽  
Vaccinium Ashei ◽  
Bud Development ◽  
Dormant Bud ◽  
Mouse Ear ◽  
Highbush Blueberries ◽  
Winter Chilling

Crop vegetative and reproductive development are frequently divided into stages to describe progression of development. Such a description is useful in denoting developmental differences between cultivars, for making crop management decisions based on growth stages, and for clear communication among individuals concerned with research, management, and production of the crop. We have developed such a scale for leaf bud development in rabbiteye blueberry (Vaccinium ashei Reade). Our scale has six stages briefly described as follows: 1) dormant bud; 2) early green tip; 3) late green tip; 4) unfolding stage; 5) mouse-ear stage; 6) fully opened bud. Categorizing buds in this manner has proven useful in comparing rates of leaf development between cultivars and in response to winter chilling. The stages appear to be relevant to highbush blueberries (V. corymbosum) as well.

A Possible Modulation Mechanism of Intramolecular and Intermolecular Interactions for NCAM Polysialylation and Cell Migration

2019 ◽  
Vol 19 (25) ◽  
pp. 2271-2282 ◽  
Author(s):  
Bo Lu ◽  
Xue-Hui Liu ◽  
Si-Ming Liao ◽  
Zhi-Long Lu ◽  
Dong Chen ◽  
...  
Keyword(s):  
Cell Migration ◽  
Intermolecular Interactions ◽  
Mammalian Cells ◽  
Molecular Mechanisms ◽  
Intramolecular Interaction ◽  
Polysialic Acid ◽  
Tumor Cell Migration ◽  
Neural Cell Adhesion ◽  
Polybasic Domains ◽  
Novel Model

Polysialic acid (polySia) is a novel glycan that posttranslationally modifies neural cell adhesion molecules (NCAMs) in mammalian cells. Up-regulation of polySia-NCAM expression or NCAM polysialylation is associated with tumor cell migration and progression in many metastatic cancers and neurocognition. It has been known that two highly homologous mammalian polysialyltransferases (polySTs), ST8Sia II (STX) and ST8Sia IV (PST), can catalyze polysialylation of NCAM, and two polybasic domains, polybasic region (PBR) and polysialyltransferase domain (PSTD) in polySTs play key roles in affecting polyST activity or NCAM polysialylation. However, the molecular mechanisms of NCAM polysialylation and cell migration are still not entirely clear. In this minireview, the recent research results about the intermolecular interactions between the PBR and NCAM, the PSTD and cytidine monophosphate-sialic acid (CMP-Sia), the PSTD and polySia, and as well as the intramolecular interaction between the PBR and the PSTD within the polyST, are summarized. Based on these cooperative interactions, we have built a novel model of NCAM polysialylation and cell migration mechanisms, which may be helpful to design and develop new polysialyltransferase inhibitors.

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