Presynaptic opioid receptors on dopaminergic nerves in the rabbit caudate nucleus: coupling to pertussis toxin-sensitive G-proteins and interaction with D2 autoreceptors?

1994 ◽  
Vol 349 (3) ◽  
Author(s):  
R. Jackisch ◽  
H. Hotz ◽  
C. Allgaier ◽  
G. Hertting
Keyword(s):  
Caudate Nucleus ◽  
G Proteins ◽  
Opioid Receptors ◽  
Pertussis Toxin ◽  
Rabbit Caudate Nucleus

scholarly journals Enkephalin activates the phospholipase C/Ca2+ system through cross-talk between opioid receptors and P2-purinergic or bradykinin receptors in NG 108–15 cells. A permissive role for pertussis toxin-sensitive G-proteins

1993 ◽  
Vol 290 (1) ◽  
pp. 241-247 ◽  
Author(s):  
F Okajima ◽  
H Tomura ◽  
Y Kondo
Keyword(s):  
Phospholipase C ◽  
G Proteins ◽  
Opioid Receptors ◽  
Pertussis Toxin ◽  
Cross Talk ◽  
Adenine Nucleotides ◽  
Single Cells ◽  
Hybrid Cell ◽  
Extracellular Atp ◽  
Permissive Role

In an NG 108-15 neuroblastoma x glioma hybrid cell suspension, extracellular ATP (via P2-purinergic receptors) and bradykinin stimulated Ins(1,4,5)P3 formation, which was accompanied by an increase in the cytosolic Ca2+ concentration ([Ca2+]i). Leucine enkephalin (EK) also slightly increased [Ca2+]i in the absence, but not in the presence, of apyrase, which hydrolyses extracellular ATP and ADP to AMP. When the cells were stimulated by P2-agonists or bradykinin prior to the application of EK, EK induces a remarkable rise in [Ca2+]i. This P2-agonist- or bradykinin-assisted EK action was also observed in single cells on a coverslip. A decrease in the extracellular Ca2+ concentration only slightly lowered the EK-induced rise in [Ca2+]i, but treatment of the cells with thapsigargin, an agent which depletes Ca2+ in the Ins(1,4,5)P3-sensitive pool, almost completely abolished EK action. The observed permissive stimulation by EK of Ins(1,4,5)P3 formation induced by a P2-agonist or bradykinin may be a primary event for the EK-induced [Ca2+]i rise. These actions of EK were antagonized by naloxone and completely reversed by prior treatment of the cells with pertussis toxin, whereas the toxin hardly affected the actions of P2-agonists and bradykinin themselves. Thus EK can induce phospholipase C activation and subsequent Ca2+ mobilization, provided that the cells have been previously or are simultaneously stimulated by endogenous adenine nucleotides or by externally applied P2-agonists or bradykinin. In this cross-talk mechanism between opioid receptors and these Ca(2+)-mobilizing agonist receptors, pertussis toxin-sensitive G-proteins play a permissive role.

FUNCTIONAL INTERACTIONS OF PERTUSSIS TOXIN-SENSITIVE G-PROTEINS WITH THE TRANSFECTED μ-OPIOID RECEPTOR IN RAT 1 FIBROBLASTS

Analgesia ◽  
1995 ◽  
Vol 1 (4) ◽  
pp. 438-441
Author(s):  
Zafiroula Georgoussi ◽  
Ian Mullaney ◽  
Alan Wise ◽  
Craig Carr ◽  
Graeme Milligan
Keyword(s):  
G Proteins ◽  
Opioid Receptor ◽  
Pertussis Toxin ◽  
Functional Interactions ◽  
Μ Opioid Receptor

scholarly journals Erratum

1991 ◽  
Vol 11 (4) ◽  
pp. 706-706
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Equilibrium State ◽  
Rat Brain ◽  
G Proteins ◽  
Pertussis Toxin ◽  
Adp Ribosylation ◽  
Gtp Binding ◽  
Reconstituted System ◽  
Α Subunits

Ischemia of Rat Brain Decreases Pertussis Toxin-Catalyzed [32P] ADP Ribosylation of GTP-Binding Proteins (Gi1 and G0) in Membranes Katsunobu Takenaka, Yasunori Kanaho, Koh-ichi Nagata, Noboru Sakai, Hiromu Yamada, Yoshinori Nozawa [ Originally published in Journal of Cerebral Blood Flow and Metabolism 1991;11:155–160] On page 158 of the above, arrows were erroneously deleted from the equation in the following passage: Heterotrimers of G proteins that bind GDP to α subunits seem to be the preferred substrates for PTcatalyzed ADP ribosylation since guanine nucleotides (GDP and GTP) and 13'Y subunits stimulate ADP ribosylation in the reconstituted system and in membranes (Tsai et aI., 1984). These results indicate that the G proteins may exist at the equilibrium state as shown below: This omission was the result of a typesetting error, which the publisher regrets.

Activation of G-Proteins by Morphine and Codeine Congeners: Insights to the Relevance of O- and N-Demethylated Metabolites at μ- and δ-Opioid Receptors

2003 ◽  
Vol 308 (2) ◽  
pp. 547-554 ◽  
Author(s):  
Chad M. Thompson ◽  
Heidi Wojno ◽  
Elisabeth Greiner ◽  
Everette L. May ◽  
Kenner C. Rice ◽  
...  
Keyword(s):  
G Proteins ◽  
Opioid Receptors ◽  
Demethylated Metabolites

scholarly journals Critical Role of Pertussis Toxin Sensitive G Proteins in the Activation of TRPC4 and TRPC5 Channels

2010 ◽  
Vol 98 (3) ◽  
pp. 326a-327a
Author(s):  
Rui Xiao ◽  
Jinbin Tian ◽  
Jisen Tang ◽  
Alexander V. Zholos ◽  
Michael X. Zhu
Keyword(s):  
G Proteins ◽  
Pertussis Toxin ◽  
Critical Role ◽  
Trpc5 Channels
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